Methods in Biotechnology
A.Y. 2018/2019
Learning objectives
The goal is to acquire knowledge and operational competencies in
genomics, functional genomics and applications to plant
improvement and to molecular taxonomy.The course is subdivided in 3 modules. The goal is to acquire knowledge and operational competencies in genomics, functional genomics and applications to plant improvement and to molecular taxonomyThe course is subdivided in 3 modules. The goal is to acquire knowledge and operational competencies in genomics, functional genomics and applications to plant improvement and to molecular taxonomy
genomics, functional genomics and applications to plant
improvement and to molecular taxonomy.The course is subdivided in 3 modules. The goal is to acquire knowledge and operational competencies in genomics, functional genomics and applications to plant improvement and to molecular taxonomyThe course is subdivided in 3 modules. The goal is to acquire knowledge and operational competencies in genomics, functional genomics and applications to plant improvement and to molecular taxonomy
Expected learning outcomes
The student will acquire competencies in generating and analyzing
data through the genomics and functional-genomics techniques.
The student will be able to plan experiments using genomic
sequencing, gene expression analyses, and their integration with
metabolomics and proteomics. The student will be able to apply
genomics approaches in molecular taxonomy, to characterize
agri-food matrices.The student will acquire competencies in generating and analyzing data through the genomics and functional-genomics techniques. The student will be able to plan experiments using genomic sequencing, gene expression analyses, and their integration with metabolomics and proteomics. The student will be able to understand and apply knowledge in molecular taxonomyThe student will acquire competencies in generating and analyzing data through the genomics and functional-genomics techniques. The student will be able to plan experiments using genomic sequencing, gene expression analyses, and their integration with metabolomics and proteomics. The student will be able to understand and apply knowledge in molecular taxonomy
data through the genomics and functional-genomics techniques.
The student will be able to plan experiments using genomic
sequencing, gene expression analyses, and their integration with
metabolomics and proteomics. The student will be able to apply
genomics approaches in molecular taxonomy, to characterize
agri-food matrices.The student will acquire competencies in generating and analyzing data through the genomics and functional-genomics techniques. The student will be able to plan experiments using genomic sequencing, gene expression analyses, and their integration with metabolomics and proteomics. The student will be able to understand and apply knowledge in molecular taxonomyThe student will acquire competencies in generating and analyzing data through the genomics and functional-genomics techniques. The student will be able to plan experiments using genomic sequencing, gene expression analyses, and their integration with metabolomics and proteomics. The student will be able to understand and apply knowledge in molecular taxonomy
Lesson period: First semester
Assessment methods: Esame
Assessment result: voto verbalizzato in trentesimi
Single course
This course cannot be attended as a single course. Please check our list of single courses to find the ones available for enrolment.
Course syllabus and organization
Single session
Responsible
Lesson period
First semester
Genomics
Course syllabus
1. Techniques and approaches in genome, exome and epigenome sequencing; 2. Strategies for plant genome analysis and assembly; 3. Plant genome structure, synteny and evolution; 4. Applications of genomics in plant breeding; 5. Basics in genome editing; 6. Workshops will be conducted on sequence analysis and alignments; design of experiments in plant genomics;
Teaching methods
The slides (in English) will be timely provided during the course and will include explanatory text; additionally, papers and books abstracts will be posted on the Ariel website of the course. Suggested text books:¿Basics on plant genomics are available in ¿Genetica e Genomica¿ (Barcaccia e Falcinelli eds.).
Functional genomics
Course syllabus
- Techniques and approaches in RNA analysis and modulation;- Strategies for genome modification;- Integration of high throughput /content techniques (omics) and data analysis;- Workshops will be conducted on gene targeting; RNA expression analysis and
Teaching methods
The slides (in English) will be timely provided during the course and will include explanatory text; additionally, papers and books abstracts will be posted on the Ariel website of the course.
Molecular taxonomy
Course syllabus
1. Species concepts: which one we use in molecular taxonomy 2. Overview of taxonomic characters 3. Principles and methods of molecular taxonomy 4. Applications of molecular taxonomy from agri-food security to biodiversity and biomonitoring 5. Molecular markers 6. Reference databases and ad-hoc built databases 7. DNA barcoding and metabarcoding: from single organism to communities 8. Single marker vs multiple markers, PCR-free gene capture approaches 9. Workshops will be conducted on experimental design, primer selection and library preparation, basic skills on bash environments, analyses of NGS data and taxonomy assignment
Teaching methods
The slides (in English) will be timely provided during the course and will include explanatory text; additionally, papers and books abstracts will be posted on the Ariel website of the course. Suggested text books:¿Molecular Plant Taxonomy - Methods and Protocols. Ed. Pascale Besse. Humana Press, 2014.¿Environmental DNA for biodiversity research and monitoring. P. Taberlet, A. Bonin, L. Zinger, E. Coissac. Oxford University Press, 2018.
Functional genomics
AGR/12 - PLANT PATHOLOGY - University credits: 3.5
Practicals: 16 hours
Laboratories: 8 hours
Lessons: 16 hours
Laboratories: 8 hours
Lessons: 16 hours
Professor:
Pasquali Matias
Genomics
AGR/07 - AGRICULTURAL GENETICS - University credits: 3.5
Practicals: 24 hours
Lessons: 16 hours
Lessons: 16 hours
Professor:
Pozzi Carlo Massimo
Molecular taxonomy
AGR/11 - GENERAL AND APPLIED ENTOMOLOGY - University credits: 3
Laboratories: 28 hours
Lessons: 10 hours
Lessons: 10 hours
Professors:
Gabrieli Paolo, Montagna Matteo
Professor(s)