Methods in Cell Biology and Biochemistry

A.Y. 2018/2019
6
Max ECTS
72
Overall hours
SSD
BIO/10 BIO/13
Language
Italian
Learning objectives
The course is meant to provide the students with:
- an in-depth overview of cellular techniques applied for the isolation, maintaining and manipulation of "in vitro" cell cultures and for the use of these cellular models in the biotechnological field;
- a state-of-the-art overview of the methodologies and approaches currently used to investigate biologically active molecules and biochemical processes; major emphasis will be given to biotechnological approaches applied in the biochemical-biomedical field.
Expected learning outcomes
Undefined
Course syllabus and organization

Single session

Lesson period
Second semester
Methods in Biochemistry
Course syllabus
Class teaching (theory and applications)
Introduction: basic concepts of statistics applied to biochemical experiments; graphic representation of experimental data; databases and softwares (e.g. NCBI, BioGPS, GO, KEGG, Panther etc.)
Approaches for the study of interactions between macromolecules
Mass spectrometry applied to the study of macromolecules
Quantitative analysis of gene expression
Approaches for the study of energy metabolism
Metabolomics techniques
Methodologies for the study of gene functions
Next generation sequencing of nucleic acids
In-person laboratory activity
Students will apply standard methods to evaluate the biochemical response to extracellular stimuli in cell cultures. Students will measure the expression of metabolic genes and readouts of oxidative mitochondrial metabolism. Students will analyze their own data by using appropriate databases and softwares.
Methods in cell biology
Course syllabus
Teaching classes
The cell culture
Primary cell culture
Immortalized cell culture
Tumor derived cells
Embryonic/adult stem cells and induced pluripotent stem cells

Methodology of cell culture
Cell culture equipment and laboratory
Cell culture conditions
Cell proliferation and adhesion assay
In vitro cell migration and invasion assay
Cell transfection
Fluorescence microscopy for examining the subcellular localization and the cellular functionality


Lab teaching
Cell culture manipulation
Study of cell viability after pharmacological treatments
Protein quantification from cellular extracts
Methods in Biochemistry
BIO/10 - BIOCHEMISTRY - University credits: 0
BIO/13 - EXPERIMENTAL BIOLOGY - University credits: 0
Practicals: 8 hours
Single bench laboratory practical: 24 hours
Lessons: 8 hours
Shifts:
Turno 1
Professor: De Fabiani Emma Selina Rosa
Turno 2
Professor: Audano Matteo
Methods in cell biology
BIO/10 - BIOCHEMISTRY - University credits: 0
BIO/13 - EXPERIMENTAL BIOLOGY - University credits: 0
Single bench laboratory practical: 16 hours
Lessons: 16 hours
Professor: Crippa Valeria
Shifts:
Professor: Crippa Valeria
Turno 1
Professor: Crippa Valeria
Turno 2
Professor: Crippa Valeria
Turno 3
Professor: Crippa Valeria
Professor(s)
Reception:
On dates and hours agreed with the interested student by phone/e-mail
Office at the Department of Pharmacological and Biomolecular Sciences (Via Balzaretti)