In Vitro Plant Breeding
A.Y. 2018/2019
Learning objectives
Knowledge of the principles, protocols and utilization of plant cell tissue culture systems, in vitro propagation and regeneration, mutagenesis and selection, secondary metabolite elicitation and cell transformation techniques, to be applied in the plant breeding programs.
Expected learning outcomes
Students who complete this course successfully will be having fundamental knowledge of plant cell culture techniques and the reasons behind the totipotency of plant cells and will be able to use plant cell and tissue culture techniques for both research and commercial purposes.
Lesson period: First semester
Single course
This course cannot be attended as a single course. Please check our list of single courses to find the ones available for enrolment.
Course syllabus and organization
Single session
Responsible
Lesson period
First semester
Course syllabus
Totipotency. Tissue Culture Methodology: sterile technique, media components, environmental effects, genetic control of culturability. Cell growth, mutation and differentiation processes in plant cultures: cell cycle, population dynamics, growth patterns, differentiation, organogenesis/somatic embryogenesis, somaclonal variation. Culture Types and Applications: undifferentiated (callus, suspension); in vitro selection, secondary metabolite production; organ, meristem and shoot micropropagation, disease elimination; germplasm preservation; embryo rescue- wide hybridization; somatic embryogenesis; artificial seeds; anther, microspore culture; homozygous line production; protoplast; gene pool diversification; plant cell cultures for plant transformation; Agrobacterium cocultivation; genome editing.
AGR/03 - ARBORICULTURE AND FRUITCULTURE - University credits: 5
Single bench laboratory practical: 8 hours
Lessons: 36 hours
Lessons: 36 hours
Professor:
De Lorenzis Gabriella
Professor(s)
Reception:
by appointment
via Celoria, 2 - Edificio 10