The course of Advanced Molecular Biology aims at providing a deep overview of the mechanisms controlling cell division and proliferation and how the cell cycle mechanisms are integrated with processes required to maintain the integrity of the genome. These topics will be discussed in model organims and in mammalian cells, with a particular focus on human pathologies linked to alterations in the molecular mechanisms of these processes.
Expected learning outcomes
Lesson period: First semester
(In case of multiple editions, please check the period, as it may vary)
- The Cell Cycle - Model organisms in cell cycle analysis - Cell cycle control systems - The control of S phase - Molecular mechanisms and control of mitosis - Completion of mitosis and cell division - Control of cell proliferation and growth - Processes that compromise genome integrity - DNA repair and DNA damage tolerance mechanisms - Pathologies related to increased genome instability - Cell cycle checkpoints in physiological and pathological conditions - The cell cycle in cancer and other human diseases. - Mechanisms responsible for the maintenance of genome integrity as targets for anticancer therapy.
Prerequisites for admission
Good knowledge of Molecular Biology, Genetics and Biochemistry is required.
PowerPoint-assisted frontal lectures. Regular attendance and active participation during classes are strongly encouraged to improve the understanding of the topics and improve communication skills. In order to facilitate active discussions, handouts will be made available before class through the Ariel platform. It is strongly recommended that students attend lectures.
-D.O. Morgan. The Cell Cycle: principles of control. New Science Press. -Friedberg, E. C., Walker, G. C., Siede, W., Wood, R. D., Schultz, R. A. & Ellenberger, T. DNA repair and mutagenesis (ASM Press). -Friedberg E.C., Elledge S.J, Lehmann A.R., Lindahl T., Muzi Falconi M. DNA Repair, Mutagenesis, and Other Responses to DNA Damage: A Subject Collection from Cold Spring Harbor Perspectives in Biology Scientific papers and reviews will be indicated during the course.
Assessment methods and Criteria
Learning assessments will be carried out by a written exam at the end of the course. Students may opt for a mid-term examination plus a second, partial exam at the end of the course. The exam includes open questions (37,5% of the final score), and multiple choices questions (62,5% of the final score). Multiple choice questions are aimed to verify the global understanding of the key concepts and definitions taught during the course, whereas open questions are designed to evaluate the ability of the student to concisely and critically describe the major cellular processes and models discussed in class. Past exam examples will be presented during the course.