Analytic methods for genes and enviroment interactions on physical exercise metabolism

A.Y. 2019/2020
6
Max ECTS
42
Overall hours
SSD
MED/46 MED/50
Language
Italian
Learning objectives
The course provides knowledge on analytical metabolic and biochemical methods for monitoring the effects of physical exercise on health and well-being. Students will learn molecular biology methods potentially useful for genetic manipulation in sports. The course provides knowledge on advanced methods for studying body metabolism and the metabolism of organs/tissues (skeletal muscle, bone, and myocardial tissue), including imaging techniques (morphology) and metabolic mapping, as well as the theoretical basics of genomics, proteomics, and metabolomics related to sports performance.
Expected learning outcomes
Students will acquire the ability to evaluate the effect of physical exercise on biochemical and chemical parameters and become acquainted with the major diagnostic tests for their evaluation; students will learn the general principles of imaging methods for studying an organism's metabolism and the metabolism of specific tissues and organs; students will learn the correlations between genomics, proteomics, and metabolomics and sports performance.
Course syllabus and organization

Single session

Lesson period
Second semester
Course syllabus
Phenotypic adaptations and genetic variability.
Gene-environment interaction; gene-DNA relationship.
From gene to protein: gene expression and protein synthesis.
Gene and chromosomal mutations.
Gene mutations and athletic performance.
Genetic variants in athletes DNA.
Gene polymorphisms and muscle mass.
Genetic polymorphisms in the DNA of athletes and the homocysteine cycle.
Technique for the study of polymorphisms: PCR.
Epigenetic control of gene expression: DNA methylation and acetylation.
Post-transcriptional regulation: role of microRNAs.
Technique for proteins identification and gene expression evaluation: Western Blotting and immunofluorescence.

Methods of conventional radiology for the study of the musculoskeletal system and hints of radioprotection.
Methods for the determination of Insulin sensitivity: insulin clamp, glycemic curve after oral and intravenous glucose loading.
Obesity and methods for studying body composition.
Changes in body composition in particular categories of patients.
Indirect calorimetry.
Sport and hydration: evaluation methods
Inflammation, environment and hormonal and metabolic alterations.
Use of insulin in diabetic subjects performing physical activity.
Gastroenteric system and sport: relationships and intervention methods.
Prerequisites for admission
Firm understanding of biology and physics and prior knowledge of elements of genetic, metabolism and endocrine physio-pathology
Teaching methods
Frontal lectures with slides projection. Attendance is compulsory
Teaching Resources
Reference material is presented during frontal lectures (slides and scientific articles). Lecture slides will be uploaded to the Ariel portal.
Assessment methods and Criteria
Oral exam: independent interview with the two course instructors (Benedini, Terruzzi). The final grade is based on the average of the grades assigned by each instructor.
MED/46 - BIOTECHNOLOGY AND METHODS IN LABORATORY MEDICINE - University credits: 3
MED/50 - APPLIED MEDICAL TECHNOLOGY AND METHODOLOGY - University credits: 3
Practicals: 12 hours
Lessons: 30 hours