Cellular communication and signal transduction

A.Y. 2019/2020
10
Max ECTS
78
Overall hours
SSD
BIO/09 BIO/10
Language
Italian
Learning objectives
The course provides fundamental informations on the principles and techniques used in protein engineering , knowledge about the cellular signaling and communication, with particular regard to the endocrine glands function and the main mechanism of hormones action they produc
Expected learning outcomes
Knowledge and understanding: the student must demonstrate that he possesses the knowledge about the principles and techniques inherent in protein engineering and signal biochemistry. In addition, knowledge concerning cellular communication, with particular regard to the main endocrine glands function and to the hormone control mechanism.
Knowledge and applied understanding: the student must demonstrate the ability to connect various topics regarding protein engineering, signal biochemistry and cellular communication, with particular attention to endocrinology, in order to be able to adequately address the training process.
Communication skills: the student must demonstrate to be able to use an appropriate terminology, which allows him to express adequately the acquired knowledge.
Ability to learn: the student will have to demonstrate to be able, in total autonomy, to increase the knowledge of the topics of the syllabus.
Course syllabus and organization

Single session

Responsible
Lesson period
First semester
Course syllabus
The course is divided into two teaching units and it aims to provide students with a broad and integrated understanding of various aspects of protein engineering, cell signalling and endocrinology.

TEACHING UNIT "PROTEIN ENGINEERING AND SIGNAL BIOCHEMISTRY":
Study of expression and function of genes
Production of recombinant proteins from heterologous systems
Site-directed mutagenesis
Rnase protection assay
Dna-protein interaction analysis
Protein-protein interaction analysis
Analysis of complex
Extracellular signals: hormones, cytokines and growth factors
receptors
Protein phosphorylation: kinases and phosphatases
Camp, adenylate cyclase and the role of g protein
Inositol phosphate metabolism and the role of other membrane lipids
Intracellular calcium: it's control and role as an intracellular signal
Nitric oxide, hydrogen peroxide and carbon monoxide

TEACHING UNIT "CELLULAR COMMUNICATION AND ENDOCRINE FUNCTION":
Intercellular communication and membrane receptors
Characteristics of endocrine functions
Synthesis and mechanical action of peptide hormones
Synthesis and mechanical action of steroid hormones
Hypothalamic hormones
Pituitary hormones
Thyroid hormones
Adrenocortical hormones
Adrenal medullary hormones
Hormonal Control of Carbohydrate Metabolism
Hormonal Control of Calcium homeostasis
Hormonal Control of energy metabolism
Prerequisites for admission
The prerequisites required to pass the exam are the knowledge of anatomy and general physiology and the most common laboratory techniques of biochemistry and molecular biology.
Teaching methods
Signal protein and biochemistry engineering: lectures and laboratory exercises.
Cellular communication and endocrine function: lectures and classroom exercises.
Teaching Resources
didactic material provided in class and available on the Ariel website.
Assessment methods and Criteria
The course is divided into two teaching units:
1) Signal protein and biochemistry engineering: written test with open questions. The final grade will take into account the accuracy and quality of the answers, as well as the communication skills.
2) Cellular communication and endocrine function: written test composed of 15 questions with 4 answers, and only one correct.
The final vote results from the weighted average of the vote reported in each teaching unit.
Cellular communication and endocrine function
BIO/09 - PHYSIOLOGY - University credits: 0
BIO/10 - BIOCHEMISTRY - University credits: 0
Practicals: 12 hours
Lessons: 18 hours
Protein engineering and biochemistry of the signal transduction
BIO/09 - PHYSIOLOGY - University credits: 0
BIO/10 - BIOCHEMISTRY - University credits: 0
Practicals: 24 hours
Lessons: 24 hours
Professor: Nonnis Simona
Professor(s)
Reception:
Monday 12.30-13.30
Department of Veterinary Medicine, Via dell'Università, 6 - 26900 Lodi, Italy