Molecular Microbiology

A.Y. 2019/2020
10
Max ECTS
78
Overall hours
SSD
BIO/19 MED/04 MED/07
Language
Italian
Learning objectives
Provide molecular tools for a comprehensive knowledge of veterinary microbiology and tools for understanding the utility of laboratory rodents as spontaneous or genetically modified disease models.
Expected learning outcomes
A) Theoretical learning
Gain knowledge on:
- Molecular mechanisms of interaction between micrrorganisms (bacteria and viruses) and the animal host.   
-Major methods to study virulence factors and strategies of antibiotic resistance and host immune system evasion.
Spontaneous, induced and genetically modified animal models and of their most common experimental applications, of the laboratory animal environment, health monitoring and strain phenotyping.
B) Practical learning
Know how to plan and perform an experiment of gene expression (from biopsy to quantification of a selected molecule).
Know how to use bioinformatic tools to interpret results of genomics and proteomic experiments.  
Knowledge of histomorphology of tissue lesions of major relevant diseases in laboratory animals (major focuns on dogs, mice and rats).
Course syllabus and organization

Single session

Responsible
Lesson period
First semester
Course syllabus
Course Description :

The number of laboratory animal models in comparative research has expanded in quantity thanks to novel techniques of rearing. Objective of the course are to provide the students of biotechnological sciences with insights to the adequate approach to the study and characterization of infectious agents and their relevance for laboratory animals The application of biotechnologies have permitted the creation of transgenic animals (GEM genetically engineered mice). New microbiological statuses have developed and their recognition and approach have changed in the last years as an example as the microbiological status of laboratory animals can interfere with results of the experiments, some agents on the contrary can not be eradicated and need to be acknowledged as possible interferences. Scientific personnel of various disciplines are involved in the production and study of laboratory animals and their microbiological status which significance is completely different from other animals. These different professions need to integrate their specialties in a team based collaboration. The course I organized in 3 Units: a) Molecular aspects of the interaction of microorganisms and hosts b) Molecular basis of microbial pathogenicity c) Experimental pathology . Units a and b will be held in Italian, unit c in English

Module: 'H52-66-A' - 'Teaching Unit: Molecular aspects of the interaction of microorganisms and hosts
Module Program
Theoretical classes:
General aspects of microorganism-host interactions
Molecular aspects of microorganism-host interactions
Receptors: the antigen recognition molecules
Bacteria-host interactions
Virus-host interactions

Practical classes:
Isolation and characterization of pattern recognition receptors

Program for not attending students :
Theoretical classes:
General aspects of microorganism-host interactions
Molecular aspects of microorganism-host interactions
Receptors: the antigen recognition molecules
Bacteria-host interactions
Virus-host interactions

Practical classes:
Isolation and characterization of pattern recognition receptors

Program's information:
Study material will be provided by the teacher in class.

Program's information for not attending students:
Study material will be provided by the teacher in class.

Module: 'H52-66-B' - "Teaching Unit: Molecular basis of microbial pathogenicity"
Program
Theoretical part:
Course Introduction
genetic mechanisms in pathogenicity
The virulence factors: techniques for the 'identification and their characteristics
Virulence and its regulation: secretion systems and quorum sensing
molecular strategies for evasion of the host's immune system
antibiotics and microbial resistance
pathogenic microorganisms in veterinary field
New strategies to control the pathogenicity : the microbiome and its modulation

EXERCISES:
bioinformatics methods applied to the study of microbial pathogenicity
SEMINARS:
the seminars will be conducted by internal researchers and / or by external collaborators working in the microbiological and related field.

[Program for not attending students with reference to descriptor 1 and 2]:
Teaching unit: Molecular basis of microbial pathogenicity:
Theoretical part:
Course Introduction
genetic mechanisms in pathogenicity
The virulence factors: techniques for the 'identification and their characteristics
Virulence and its regulation: secretion systems and quorum sensing
molecular strategies for evasion of the host's immune system
antibiotics and microbial resistance
pathogenic microorganisms in veterinary field
New strategies to control the pathogenicity : the microbiome and its modulation

EXERCISES:
bioinformatics methods applied to the study of microbial pathogenicity
SEMINARS:
the seminars will be conducted by internal researchers and / or by external collaborators working in the microbiological and related field.
Teaching material:
Readings and teaching material for the exam are provided by the teacher in class (copies, pdf,ppt)

Module: 'H52-66-C' - 'Unit: Experimental Pathology'.
Presentation:
The number of laboratory animal models in comparative research has expanded in quantity thanks to the application of biotechnologies to laboratory rodents that have permitted the creation of transgenic animals. Scientific personnel of various disciplines are involved in the production and study of laboratory animal models. These different professions need to integrate their specialties in team based collaboration.

COURSE OBJECTIVES:
Objective of the course is to provide the students of biotechnological sciences with insights to the adequate approach to the comprehension of most utilized animal models . The course will illustrate and explain major comparative spontaneous and experimental models of disease from the perspective of pathology
ORGANIZATION OF THE COURSE:
The course will be held by the responsible aided by pathologists working in different fields with laboratory animals (academia, private research centers, pharmaceutical industry, contract laboratories).

LECTURES:
· The role of the veterinary pathologist in laboratory animal studies
· Most utilized animal models.
· Transgenic mice: production and characterization.
· Transgenic mouse models of infectious diseases.
· Principles of phenotyping of transgenic mice
· Nomenclature of mouse strains and genetically modified mice.
· Main spontaneous diseases (environmental, aging, infectious, neoplastic) in laboratory rodents
· Main diseases of rodent laboratory strains
· Immune deficient mice: infectious diseases

PRACTICAL TEACHING:
Most practical teaching will be performed at the microscope and seminars held by specialists of the filed.
Prerequisites for admission
Undergraduate three year university programmes.
Recommended (not mandatory): Knowledge of basic anatomy, basic histology, basic physiology and basic laboratory methods.
Teaching methods
Frontal teaching, Seminars and Practicals in the laboratory and at the microscope
Teaching Resources
Study material will be provided to the students by the professors as hard copies, pdf files ppt files and posted in the web site ARIEL.
Assessment methods and Criteria
The exam will be written and provided for all sectiosn contemporarily. The exam will consist of multiple choice questions (majority) and short essay type questions (minority). Enrollment trough SIFA is mandatory to access the exam.
Unit: Experimental Pathology
BIO/19 - MICROBIOLOGY
MED/04 - EXPERIMENTAL MEDICINE AND PATHOPHYSIOLOGY
MED/07 - MICROBIOLOGY AND CLINICAL MICROBIOLOGY
Practicals: 12 hours
Lessons: 18 hours
Professor: Roccabianca Paola
Unita' didattica: Aspetti molecolari dell'interazione microrganismo-ospite
BIO/19 - MICROBIOLOGY
MED/04 - EXPERIMENTAL MEDICINE AND PATHOPHYSIOLOGY
MED/07 - MICROBIOLOGY AND CLINICAL MICROBIOLOGY
Practicals: 12 hours
Lessons: 12 hours
Professor: Turin Lauretta
Unita' didattica: Basi molecolari della patogenicità microbica
BIO/19 - MICROBIOLOGY
MED/04 - EXPERIMENTAL MEDICINE AND PATHOPHYSIOLOGY
MED/07 - MICROBIOLOGY AND CLINICAL MICROBIOLOGY
Practicals: 12 hours
Lessons: 12 hours
Professor: Soggiu Alessio
Professor(s)
Reception:
e.mail appointment
DIMEVET
Reception:
Monday 9:00 a.m. - 1 p.m.
Polo Lodi