Cellular regeneration techniques

A.Y. 2020/2021
Overall hours
Learning objectives
The course will provide the students with a detailed knowledge of the molecular and cellular principles that regulate embryonic development. This knowledge will be extended to the biology of stem cells both tissue specific and embryonic. The course will include some information on the most recent technologies of cell conversion. Finally, the possible therapeutic application of these cells will be critically assessed.
Expected learning outcomes
At the end of the course, the student acquires a solid knowledge of the molecular and cellular principles controlling embryonic development. This knowledge finds applications to the biology of embryonic and adult stem cells, as well as to the most recent technologies of cell conversion, with their possible therapeutic applications.
Course syllabus and organization

Single session

Lesson period
First semester
Asynchronous lectures will be provided (videos of slides with audio commentary), organized to cover the topics of each lesson of the course. Recorded lectures will be made available on the ARIEL platform. Synchronous meeting will be periodically scheduled via Microsoft TEAMS platform to provide clarifications and insights. Exams will take place on line, in the same way as those taken in person.
Course syllabus
{Modulo}: 'H52-54-A' - 'Unit 1: Regenerative medicine, biological principles and laboratory procedures'.

Stem cells: biological principles 2 hours
Embryonic stem cells (ESC): origins and biological characteristics 2 hours
The molecular mechanisms that regulate pluripotency of ESC 2 hours
Cellular reprogramming 2 hours
iPS cells 2 hours
Cell conversion 2 hours
Mesenchymal stem cells 2 hours
Intestinal stem cells 2 hours
Neural stem cells 2 hours
Student presentations on topics of their choice and discussion 6 hours

{Modulo}: 'H52-54-B' - 'Unit 2: Molecular Embryology'.
Key molecules in early embryo development
Trofectoderm and inner cell mass. Transcription factors controlling cell plasticity
Imprinting and epigenetic regulation
Maternal and paternal contribution to the mitotic spindle, centromere and centrioles
Cytoplasm compartimentalization
Telomerase and cell death
Epigenetics and cell fate definition
Developmental cues, gradients, morphogenesis
Genome editing (siRNA, ZFN, Talens, Crispr/Cas)
Prerequisites for admission
Courses of Istology, Anatomy, Physiology and Embryology
Teaching methods
Lectures, student presentations and practical classes in the lab
Teaching Resources
Handouts and updated scientific publications available to the students
Assessment methods and Criteria
The examination takes place as a single written examination for each module consisting of twenty multiple-choice questions (5 answers) with the possibility of choosing only one or more correct answers.
The student must reach at least 18/30 in each module.
The final mark of the two written tests, expressed in thirtieths, corresponds to the weighted average of the two written test.
Course work consisting of a 15 minutes presentation and a 1000 words essay on a topic of choice will count for up to 40% of the final mark.
Exam are in number of 8 in the Academic year and take place in the months of January, February, April, June, July, September, October and December. There are no additional calls for out-of-course. To take the exam, it is essential to register for the exam through the ONLINE SIFA service of the University.
Unita' didattica: Embriologia molecolare, dalla cellula all'apparato
VET/01 - VETERINARY ANATOMY - University credits: 3
Practicals: 12 hours
Lessons: 12 hours
Professor: Brevini Tiziana
Unita' didattica: Medicina rigenerativa: principi biologici e procedure di laboratorio
VET/01 - VETERINARY ANATOMY - University credits: 4
Lessons: 24 hours
Professor: Brevini Tiziana