Drug analysis 2 and laboratory of drug analysis 2

A.Y. 2020/2021
Overall hours
Learning objectives
The lectures and the individual laboratory training of Drug Analysis II and Laboratory of Drug Analysis II are aimed at providing the student with the knowledge and the practice of chemical and instrumental methods for the identification of the substances reported in European Pharmacopoeia monographs, improving his critical skills in approaching analytical challenges in the pharmaceutical field.
Expected learning outcomes
At the end of the course, the student will be able to understand the theoretical aspects of the principal techniques of identification and analysis of pharmaceutical substances and to properly apply them so as to answer the questions of the conclusive written examination.
Course syllabus and organization

Single session

Lesson period
The lessons will be by remote on the Teams platform. They will be video-registered and made available on the Ariel platform. The individual laboratory training will consist in 20 hours of training in laboratory, 16 hours of training by video-registered laboratory experiences and 10 hours of lessons by remote.
If possible, the exam will be a written test in the manner described for the previous accademic year. Otherwise, the exam will consist in an oral test of about 30 minutes on the Teams platform.
Course syllabus
- Introduction to drug analysis - Qualitative and quantitative elemental analysis of organic compounds - Tests to identify organic chemical functions: alcohols, polyalcohols, phenols, ketones and aldehydes, sugars, carboxylic acids and derivatives, amines, amino acids, heterocyclic compounds - Tests to identify classes of compounds of medicinal interest.
Solubility, partition coefficient, extraction techniques, ternary solubility diagrams - The solid state and its characterization (melting point, polymorphism, binary phase diagrams) - The liquid state and its characterization (boiling point, fractional distillation, phase diagrams of binary liquid mixtures) - Thermal analysis (TGA, TA DSC) - Refractometry - Polarimetry - Circular dichroism - Chiral compounds: chiroptical properties and analytical methods to determine enantiomeric composition - NIR spectroscopy and PCA analysis.

Individual laboratory training

Recognition of organic and metallorganic substances of pharmaceutical interest
Introduction to the analysis of organic compounds. Organoleptic examination. Combustion tests and residue analysis, recognition of metals in organometallic substances registered in the F.U.
Preparation of Lassaigne's solution for the detection of nitrogen, sulfur, halogens, phosphorus. Solubility of organic substances: solubility in water and reactive solvents; classification of substances based on their solubility.
Assays for the recognition of functional groups in organic substances: alcohols, aldehydes, ketones, polyalcohols, sugars, carboxylic acids, α-oxyacids, phenols, amines, amino acids, esters, amides.
Examination of the substances registered in the F.U. Pharmaceutical substance classes: general and specific tests for alkaloids, sulpha, xanthine, glycosides, various substances.
Recognition of inorganic substances of pharmaceutical interest.
Prerequisites for admission
To conveniently attend the lessons, the student should possess basic knowledge of organic chemisty, medicinal chemistry and analytical chemistry.
Teaching methods
Traditional lessons supported by slides (available in Ariel).
Individual laboratiry training.
Teaching Resources
- Slides available in Ariel - V. Cavrini - Guida al riconoscimento di composti di interesse farmaceutico - Esculapio Bologna - Vogel - Chimica Organica Pratica - Casa Editrice Ambrosiana - D. G. Watson - Analisi Farmaceutica - EdiSES s.r.l. Napoli - H. H. Bauer, G. D. Christian, J. E. O'Relly - Analisi strumentale - Piccin, Padova - R. Cozzi, P. Protti e T. Ruaro - Analisi chimica moderni metodi strumentali Vol I, II e III - Zanichelli Bologna. - R. Ugo - Analisi chimica strumentale - Editrice Scientifica Guadagni, Milano. - Kenneth A. Connors - A textbook of pharmaceutical analysis - John Wiley & Sons - F. Savelli, O.Bruno - Analisi Chimico Farmaceutica - Piccin
Assessment methods and Criteria
Individual laboratory training: attendance is assigned if 50% analyses are correct and it is valued from 1/30 to 6/30 points depending on the results of the analyses. These points add to the points resulting from the final written exam, whose top score is 24/30.

The final written exam has the following characteristics:

It includes (i) open questions (short answers are required in the prefixed number of lines reported in the exam format) and (ii) multiple choice questions dealing with all topics covered by the course by which the student should demonstrate his knowledge and understanding as well as his capacity to discuss the key concepts of each topic. The mark of the exam will be rapidly recoded and the student could accept or neglect this within the provided deadline. Before this deadline, a meeting will be scheduled during which the students can analyze and discuss the results of their exam.

During a solar year, there will be seven exams and the students can take consecutive exams without any constraining.
CHIM/08 - PHARMACEUTICAL CHEMISTRY - University credits: 6
Lessons: 48 hours
Professor: Pallavicini Marco
CHIM/08 - PHARMACEUTICAL CHEMISTRY - University credits: 4
Single bench laboratory practical: 64 hours
Turno 2
Professor: Bolchi Cristiano
Turno 1
Professors: Bolchi Cristiano, Pallavicini Marco
Turno 3
Professor: Villa Stefania