Methods in Cytochemistry

The aim of the course is to provide students with the basic principles of cytochemistry and histochemistry. The preparation techniques for sample analysis used in cytochemistry, histochemistry, immunocytochemistry and in situ hybridization will be illustrated. Basic knowledge will also be provided for optical and electronic visualization of biological interest macromolecules. Theoretical lessons will be associated with seminars / workshops relating to examples of application of these methods to the study of cellular biology and instrument observation tools.

At the end of the course, the student will have acquired basic theoretical knowledge related to:
- preparation techniques of histological samples and their cyto-histochemical staining;
- main techniques of immunohistochemistry and in situ hybridization;
- main methods of in situ observation of biological macromolecules in cells and tissues.