Molecular biology applied to the biomedical research

A.Y. 2020/2021
Overall hours
Learning objectives
The first part of the course provides a detailed overview of the molecular mechanisms of cell cycle control. The second part will focus on the structure of the genome and the molecular mechanisms that regulate genome stability and avoid cancer onset and progression. Human diseases resulting from defects in the mechanisms of cell cycle control and in processes responsible for maintaining the integrity of the genome will be taken into consideration.
Expected learning outcomes
At the end of the course, students will acquire knowledge about the following topics:
· Cell cycle progression and its control;
· Mitosis and meiosis;
· Genome stability maintenance.
· Tumor onset and progression;
· Molecular mechanisms altered in many genome instability diseases.
Course syllabus and organization

Single session

Lesson period
Second semester
Asynchronous lessons (PowerPoint video lessons) will be made available, organized to cover the topics of each lesson of the teaching. The recorded lessons will be made available to students on the ARIEL teaching platform throughout the semester and uploaded to the site according to the teaching schedule. Furthermore, once a week, always according to the teaching timetable, a synchronous meeting with the students will be organized, using the Microsoft Teams platform, in order to provide clarifications and insights on the teaching topics and answer students' questions.

The program and reference material will not be affected.

Should the situation make it necessary, the written exams will be carried out remotely using the platform, in the manner illustrated on the University portal. The written test will have the same structure as the test carried out in the classroom: 4 open questions, duration 1h and 20 min. Even in an emergency phase, students who regularly attend lessons can take the exam by passing an ongoing written test and one at the end of the course. Both tests will have an identical structure to that indicated for the general exam and the final grade will be the average of the marks obtained in the two tests. Evaluation parameters: the student's knowledge, their logic and reasoning skills will be evaluated.
Course syllabus
The eukaryotic Cell Cycle:
- Model organisms in the cell cycle analysis;
- The cell cycle control systems;
- The control of S phase;
- Early mitosis: preparing the chromosomes to segregation;
- Assembly of the mitotic spindle;
- The completion of mitosis and the cytokinesis;
- The control of meiosis;
- Control of cell proliferation and growth;
- The checkpoints of the cell cycle in physiological and pathological growth conditions;
- Alterations of cell cycle in cancer and other human diseases.

The maintenance of genome stability:
- The eukaryotic chromosomes: structure and organization, centromeres and telomeres;
- Alteration of the structure and number of chromosomes;
- Different types of DNA damages;
- Essential molecular mechanisms that preserve the correct structure of chromosomes;
- A plethora of DNA repair pathways;
- DNA damage checkpoints that control genomic stability;
- Human disorders whose cause is a defect in the response to or repair of DNA damage;
- Molecular mechanisms responsible for the development of the immune system;
- The molecular pathways involved in the maintenance of genomic stability as targets of therapeutic treatments
Prerequisites for admission
Good knowledge of Genetics and Molecular Biology is required.
Teaching methods
Teaching Methods:
Front lectures supported by projected slides.
The students will be encouraged to actively participate in the discussion to improve their critical skills.
Language: Italian
Program information:
The lessons in pdf format will be available on the ARIEL website of the teacher.
WEBsite: ARIEL website of the teacher.
Teaching Resources
D.O. Morgan. "The Cell Cycle: principles of control". New Science Press, 2007.
Friedberg, E. C., Walker, G. C., Siede, W., Wood, R. D., Schultz, R. A. & Ellenberger, T. (2005) "DNA repair and mutagenesis" (ASM Press).
Robert Weinberg. "La biologia del cancro", 2016
Tak W. Mak, Mary Saunders "Fondamenti di immunologia", 2017
F. Amaldi, P. Benedetti, G. Pesole, P. Plevani, "Biologia Molecolare" Casa Editrice Ambrosiana.
J.D. Watson, T.A Baker, S.P. Bell, A. Gann, M. Levine and R. Losick "Molecular Biology of the Gene"
Research and review articles concerning the topics dealt with during lectures will be suggested.
Assessment methods and Criteria
Written examination (4 open questions, duration 1h and 20 min); students who regularly attend classes can take the exam by passing one written test in progress at the end of the first half of the program and one at the end of the course. Both tests will have an identical structure to that indicated for the general exam and the final grade will be the average of the marks obtained in the two tests. Assessment parameters: the student's knowledge, their logic and reasoning skills will be assessed. Method of communicating the results: following official reporting of the results, the students will receive an e-mail from the system in which they will be informed of their mark which they can accept or reject.
BIO/11 - MOLECULAR BIOLOGY - University credits: 6
Lessons: 48 hours
Professor: Marini Federica
Tuesday, Friday 14:30-16:00
Via Celoria 26, 4th floor