Functional genomics and the molecular basis of differentiation

A.Y. 2021/2022
8
Max ECTS
66
Overall hours
SSD
AGR/17 VET/06
Language
English
Learning objectives
The objective of the course is to provide students with: 1) a general overview on the principal methods for the study of bacterial genomes (genome sequencing techniques, genome annotation, phylogenetic studies); 2) a general overview on metagenomics and meta-trancriptomics analyses; 3) a practical approach to LINUX for genomes annotation and sequencing analyses.
In addition, the course provides students with information and training in the advances in the understanding of the molecular basis of genomic and epigenetic mechanisms in gene expression in livestock species. In detail the objective of the course is to provide students with 1) a general overview of the OMIC technologies used to study the primary structure of DNA, 2) detailed knowledge of the use of OMIC technologies in epigenetics 3) a general overview of epigenetics and role of epigenetic changes in development and cell differentiation
Expected learning outcomes
At the end of the course, the student will to be able to plan experiments for the study of microbial communities, genomes sequencing and annotation and phylogenetic relationship between organisms. The practical approach of the course will introduce the student to bioinformatics with the use of tools capable to study genomes and the interaction between organisms.
In addition, the students will acquire knowledge on epigenetics changes on DNA and their effect on gene regulation and cell differentiation. Furthermore, the students will have a detailed overview on the new techniques applied to epigenetic profiling and acquire expertise on ENCODE database
Course syllabus and organization

Single session

Responsible
Lesson period
First semester
More specific information on the delivery models of training activities for academic year 2021/22 will be provided over the coming months, based on the evolution of the public health situation
Course syllabus
Teaching unit: Genomics and molecular basis of differentiation in model invertebrate organisms and pathogens
1) An overview on the principal molecular biology methods
2) Sanger sequencing
3) A general overview on the principal methods for the study of bacterial genomes (genome sequencing techniques, genome annotation, phylogenetic studies);
4) a general overview on metagenomics and meta-trancriptomics analyses;
5) a practical approach to bioinformatics tools
Teaching unit: Genomics and epigenetics in vertebrates
Introduction to OMIC technologies in Livestock
Next Generation sequencing applied to expression studies in Livestock
Epigenetic Theory
Epigenetics of Mammalian gamete and embryo development
Epigenetic modifications; Methylation, Acetylation and Chromatin remodelling
Roles of Imprinted genes
Epigenetics and Animal Health
Epigenetics and MicroRNAs
Prerequisites for admission
Knowledge required for the access to the Degree Course.
Teaching methods
Teaching unit: Genomics and molecular basis of differentiation in model invertebrate organisms and pathogens
Lectures, practical laboratory activities, group work.
Teaching unit: Genomics and epigenetics in vertebrates
The course is organized in lectures and practical exercise.
Lectures are based on slides and presentations.
Practical exercises are based exploring databases and on exercises based on database information.
Journal clubs are organized on scientific papers as individual or group work.
Teaching Resources
Teaching unit: Genomics and molecular basis of differentiation in model invertebrate organisms and pathogens
Material provided by the teacher and uploaded to the Ariel platform.
Teaching unit: Genomics and epigenetics in vertebrates
1) Livestock Epigenetics by Hassan Khatib, Published by John Wiley and Sons
2) Genetica Animale, Giulio Pagnacco, Seconda Edizione.
Assessment methods and Criteria
Teaching unit: Genomics and molecular basis of differentiation in model invertebrate organisms and pathogens
The learning assessment will be carried out through a written test: 11 multiple choice questions
Teaching unit: Genomics and epigenetics in vertebrates
The assessment is based on a written assay.
Unit: Genomics and epigenetics in vertebrates
AGR/17 - LIVESTOCK SYSTEMS, ANIMAL BREEDING AND GENETICS - University credits: 0
VET/06 - PARASITOLOGY AND ANIMAL PARASITIC DISEASES - University credits: 0
Practicals: 12 hours
Lessons: 18 hours
Professor: Minozzi Giulietta
Unit: Genomics and molecular basis of differentiation in model invertebrate organisms and pathogens
AGR/17 - LIVESTOCK SYSTEMS, ANIMAL BREEDING AND GENETICS - University credits: 0
VET/06 - PARASITOLOGY AND ANIMAL PARASITIC DISEASES - University credits: 0
Practicals: 24 hours
Lessons: 12 hours
Professor: Bazzocchi Chiara