Innovative molecular approaches for the identification of pharmacological targets

A.Y. 2021/2022
8
Max ECTS
64
Overall hours
SSD
BIO/10
Language
Italian
Learning objectives
The aim of the course is to provide students with the scientific knowledge that will contribute to the
formation of a basic culture of omics technologies and to the learning of the language to be used.
The aim of the course is to allow students to understand the fundamental logic in the use of omic
methodologies, to know the theoretical and practical bases and the principles on which the different
omic techniques are based, the main protocols of use both in the preparation of the sample and of
the instrumentation and their control, and finally to provide the basis for the management and analysis of the results also illustrating the possibility of integration of the data obtained with different omic methodologies.
The educational objective of the course is to develop knowledge that allows the student to address different scientific issues and to choose and apply the best omics approaches in order to understand the biochemical functions in the main tissues or cellular models in relation to the most common diseases.
Expected learning outcomes
The students' learning outcomes will be evaluated by the teacher through an oral examination of the learning of the omic methodologies and their use on the basis of the program carried out during the course.
The examination of the students by the teacher will be mainly aimed at assessing the knowledge and understanding of the omic techniques and the assessment of the skills acquired by the student at the completion of the teaching itself. In particular, the ownership of the language used
by the student will be evaluated, as well as the skills acquired to discriminate the choice of different omic methodologies and their possible integration to be applied to different scientific issues.
Course syllabus and organization

Single session

Lesson period
Second semester
Prerequisites for admission
Basic knowledge in Biochemistry and Applied Biochemistry are strongly recommended.
Assessment methods and Criteria
The students' learning outcomes will be evaluated by the teachers through an oral examination of the learning of the program carried out during the course.
Molecular biology applied to the study of new druggable targets
Course syllabus
The following topics will be explained during the section 1:
- Introduction to genomics
- Sequencing technology platform
- Single Nucleotide polymorphisms (SNPs), exome sequencing and genome wide association
study (GWAS)
- DNA-protein interaction at the genome level (chromatin immunoprecipitation coupled to
next generation sequencing-ChIP-Seq)
- Functional genomics screens
- Epigenomics
- Metagenomics
- Introduction to transcriptomics
- Nascent RNA and total RNA sequencing
- Splicing analysis
- Other RNAs analysis (miRNAs, long non-coding RNAs, small nuclear RNAs and others)
Teaching methods
The course Innovative molecular approaches for the identification of pharmacological targets is composed of two sections : 1. Molecular biology techniques in the studies of new therapeutic targets (32 h) and 2. New techniques in proteomics (32 h). Both sections consists of lectures given by the Teacher and occasionally seminars by external experts on particular subjects.
Teaching Resources
Power point presentations or short videos are usually used and are provided to the students in the Ariel site of the course.
Current proteomic technologies
Course syllabus
The following topics will be explained during the section 2:
- Introduction to proteomics
- Proteomic platform and instrumentation (high resolution nanoLC-MS/MS system)
- Labelled and label-free proteomics
- Imaging proteomics and applications
- Structural proteomics
- Introduction to bioinformatics
- Omics data integration
Teaching methods
The course Innovative molecular approaches for the identification of pharmacological targets is composed of two sections : 1. Molecular biology techniques in the studies of new therapeutic targets (32 h) and 2. New techniques in proteomics (32 h). Both sections consists of lectures given by the Teacher and occasionally seminars by external experts on particular subjects.
Teaching Resources
Power point presentations or short videos are usually used and are provided to the students in the Ariel site of the course.
Current proteomic technologies
BIO/10 - BIOCHEMISTRY - University credits: 4
Lessons: 32 hours
Molecular biology applied to the study of new druggable targets
BIO/10 - BIOCHEMISTRY - University credits: 4
Lessons: 32 hours