Methods in molecularbiology

A.Y. 2021/2022
Overall hours
Learning objectives
The purpose of this course is to train students in the main molecular biology methods. The treatment of such methods is based on the principles on which the techniques are based, explaining the experimental results and their interpretation. There are also numerous examples of applications in academic and industrial research and medical and diagnostic fields.
Expected learning outcomes
At the end of the course, the student will have to know the theoretical and practical principles to start working successfully in a molecular biology laboratory or where molecular biological techniques are used. The student will also be able to understand the techniques used in detail and apply them appropriately.
Course syllabus and organization

Single session

Lesson period
First semester
More specific information on the delivery modes of training activities for the academic year 2021/22 will be provided over the coming months, based on the evolution of the public health situation.
Course syllabus
- Basic Techniques of Analysis (DNA/RNA Electrophoresis and variants, Southern/Northern blot, PCR, DNA cloning )
- Genomic/cDNA Library cloning vector and applications
- Genetic screening, phage display, two hybrid and variants
- DNA sequencing technologies
- Model organisms
- Synthetic lethality screening and application as therapeutic strategies
- Mutants
- Methods to study genic expression
- Protein-Nucleic Acid interaction
- During the course several examples of research published in scientific journals are discussed.
- The course includes 16 hours of laboratory exercises on the topics concerning the course.
Prerequisites for admission
Students are expected to have basic knowledge of biochemistry, molecular biology.
Teaching methods
Frontal teaching with a high level of teacher interaction supported by projected teaching material which is available to students from a dedicated website. Extensive discussions to allow development of critical faculties and encourage constructive individual involvement in the teaching/learning process.
Teaching Resources
Richard J Reece
Analisi dei geni e dei genomi

T.A. Brown
Genomi 3
Materials provided in lecture
Assessment methods and Criteria
Exams are written tests consisting of open questions.
BIO/11 - MOLECULAR BIOLOGY - University credits: 6
Lessons: 48 hours
Professor: Lazzaro Federico