Research strategies and methodologies applied to disease study and control

A.Y. 2021/2022
8
Max ECTS
66
Overall hours
SSD
VET/05 VET/06
Language
Italian
Learning objectives
The main objectives of the course are: to provide students with information on the main traditional and advanced biomolecular techniques to be used in the parasitological field; to provide students with concepts related to the transformation, use and management of transgenic mice.
In details, the course provides students with concepts related to the qualitative and quantitative PCR to be applied in molecular parasitology. The practical lessons will allow students to work in a molecular biology laboratory. In addition, the course provides students with concepts related to the transformation of primary cell or cell lines and to the generation and management of genetically modified mice.
Expected learning outcomes
At the end of the course, students acquire a solid knowledge for experimental planning of the application of molecular diagnostic tools in the field of parasitology. Students will also acquire the critical capacity to comment the obtained results and to resolve any technical problems arising from the molecular analyzes carried out.
At the end of the course, the student acquires a solid knowledge of experimental planning with genetically modified murine and cell models. This knowledge finds applications to the use experimental animals in particular to the management of animal facilities, in vivo and in vitro experimental planning, writing projects for in vivo experimentation approval.
Course syllabus and organization

Single session

Responsible
Lesson period
First semester
More specific information on the delivery models of training activities for academic year 2021/22 will be provided over the coming months, based on the evolution of the public health situation
Course syllabus
1) Teaching unit "Advanced biotechnology in parasitology"
Reading scientific papers and writing material & methods;
Designing research projects;
How to handle and store biological samples;
Research strategies; molecular biology and parasitology landmarks
Principal molecular biology techniques (qualitative and quantitative PCR);
Recombinant proteins for immunological studied and vaccines productions.
2) Teaching unit " Genetically modified mouse and cells models "
Introduction to animal models
Legislation on in vivo experimentation
Immunobiology of mouse
Transgenesis
Gene targeting and conditional gene targeting
Management of genetically modified mouse colonies
In vivo imaging
Genetically modified cell lines
Prerequisites for admission
1) Teaching unit "Advanced biotechnology in parasitology"
Knowledge required for the access to the Degree Course.
2) Teaching unit " Genetically modified mouse and cells models "
Inorganic and organic chemistry
Cellular biology
Physic
Molecular biology
Genetic
Teaching methods
1) Teaching unit "Advanced biotechnology in parasitology"
Lectures, practical laboratory activities, group work.
2) Teaching unit " Genetically modified mouse and cells models "
The course is developped by frontal lessons and practical exercise.
Frontal lessons take advantages on presentations, videos, and seminars of expert collegues.
Practical exercises contemplate lab experiences, visits to companies or institutions of the area (animal facilities, companies that produce animals or instrument for animal facilities), search and study of scientific papers. The journal club presentation is organized as group work.
Teaching Resources
Teaching unit "Advanced biotechnology in parasitology"
Material provided by the teacher and uploaded to the Ariel platform.
Teaching unit " Genetically modified mouse and cells models "
1) Handbook of Veterinary Immunology, ed. Pastoret-Griebel-Bazin-Govaerts (Academic Press)
2) Gene Targeting: a practical approach, ed. A.I. Joyner Oxford University Press
3) Microbiologia e Immunologia Veterinaria, Poli et al., ed. UTET
4) Benessere e animal care degli animali da laboratorio, Garattini Grignaschi, Luzi, Riva, ed. Point Veterinaire Italie
5) Gazzetta Ufficiale DL 26/2014
Assessment methods and Criteria
1) Teaching unit "Advanced biotechnology in parasitology"
The learning assessment will be carried out through an oral test. The test will consist in the presentation of a scientific article followed by questions to verify the level of learning on the topics taught.
2) Teaching unit " Genetically modified mouse and cells models "
The assesment method consist in an oral examination. The student should properly expose the arguments of the course. The criteria of the evaluation include: explaination capacity, course argument comprehension validation, capability to plan simple experimentations related to the course, critic capacity of scientific papers, capability to prepare an oral presentation of a scientific paper.
Unita' didattica: Biotecnologie avanzate in parassitologia
VET/06 - PARASITOLOGY AND ANIMAL PARASITIC DISEASES - University credits: 5
Practicals: 24 hours
Lessons: 18 hours
Professor: Bazzocchi Chiara
Unita' didattica: Modelli murini e cellulari geneticamente modificati
VET/05 - INFECTIOUS DISEASES OF DOMESTIC ANIMALS - University credits: 3
Practicals: 12 hours
Lessons: 12 hours
Professor: Riva Federica
Professor(s)
Reception:
Wednesday; 11.00-12.00
DIVET; General Pathology and Parasitology