Biochemistry
A.Y. 2022/2023
Learning objectives
At the end of the course the student will be able to describe the fundamental concepts on how the cell functions, based on its architecture, (macro)molecular components, metabolism, and the role of genetic information. Students will acquire an operative understanding of biochemistry, which will enable them to work within a bioinformatics context, with full comprehension of the key molecular players involved.
Expected learning outcomes
Students will acquire an operative understanding that will enable them to work within a bioinformatics context, with full control and understanding of the acting molecular partners and their interactions.
Lesson period: First semester
Assessment methods: Esame
Assessment result: voto verbalizzato in trentesimi
Single course
This course cannot be attended as a single course. Please check our list of single courses to find the ones available for enrolment.
Course syllabus and organization
Single session
Responsible
Lesson period
First semester
Course syllabus
The following course topics will be covered:
Introduction to biological macromolecules. Proteins as polymers of amino acids; nucleotides in relation to energetic considerations; nucleic acids as polymers of nucleotides; polysaccharides and lipids (basic).
Protein structure: the peptide bond, Ramachandran plot, secondary and higher-level structures. Protein Data Bank (PDB), computational biology and databases, macromolecular dynamics and their simulations.
Protein functions, enzymes: catalysis, enzyme kinetics (basic), role of catalysis in metabolism.
Thermodynamics of metabolism. Biochemical thermodynamics; ATP, energy transfer, coupling of endergonic/exergonic reactions.
Energy metabolism. Form glucose to CO2 : Glycolysis, pyruvate oxidase, tricarboxylic acid cycle, electron transfer, oxidative phosphorylation.
General concepts. Linking of the above concepts to cell functionality; examples of metabolism-related pathologies.
Introduction to biological macromolecules. Proteins as polymers of amino acids; nucleotides in relation to energetic considerations; nucleic acids as polymers of nucleotides; polysaccharides and lipids (basic).
Protein structure: the peptide bond, Ramachandran plot, secondary and higher-level structures. Protein Data Bank (PDB), computational biology and databases, macromolecular dynamics and their simulations.
Protein functions, enzymes: catalysis, enzyme kinetics (basic), role of catalysis in metabolism.
Thermodynamics of metabolism. Biochemical thermodynamics; ATP, energy transfer, coupling of endergonic/exergonic reactions.
Energy metabolism. Form glucose to CO2 : Glycolysis, pyruvate oxidase, tricarboxylic acid cycle, electron transfer, oxidative phosphorylation.
General concepts. Linking of the above concepts to cell functionality; examples of metabolism-related pathologies.
Prerequisites for admission
None
Teaching methods
Lessons will consist of frontal lectures, according to the course timetable. Active participation in lessons is strongly encouraged.
Teaching Resources
Suggested text books:
- Appling, Antony-Cahill & Mathews: Biochimica, Molecole e Metabolismo (Pearson 2017)
- Nelson D.L. & Cox M.M. - Lehninger's Principles of Biochemistry, Worth Publishers
- Voet D. & Voet, J.G. - Biochemistry, Wiley & Sons.
Additional learning materials (animations, websites and scientific articles) will be indicated and provided by the teacher during the course.
- Appling, Antony-Cahill & Mathews: Biochimica, Molecole e Metabolismo (Pearson 2017)
- Nelson D.L. & Cox M.M. - Lehninger's Principles of Biochemistry, Worth Publishers
- Voet D. & Voet, J.G. - Biochemistry, Wiley & Sons.
Additional learning materials (animations, websites and scientific articles) will be indicated and provided by the teacher during the course.
Assessment methods and Criteria
Exams will consist of multiple-choice questions, to assess global understanding of the entire course and open questions, to assess in-depth knowledge of key concepts presented during the course.
Professor(s)
Reception:
Upon request
Towerblock 5B, Dep. Biosciences, Via celoria 26, 20133