Methods in Cytogenetics and Human Genetics
A.Y. 2022/2023
Learning objectives
The course aims to provide the educational and methodological bases that allow to understand the normal and pathological genetic variability in humans. Another aim of the course is to provide basic knowledge of the structure of the human genome and the molecular mechanisms underlying the processes that regulate gene expression.
Expected learning outcomes
At the end of the course, the student will have acquired:
- the most recent methods of cytogenetics and analysis of the structure of the human genome;
- the theoretical and conceptual bases of human genetics and of genetic diseases
- the main molecular mechanisms related to the control of gene expression
- the most recent methods of cytogenetics and analysis of the structure of the human genome;
- the theoretical and conceptual bases of human genetics and of genetic diseases
- the main molecular mechanisms related to the control of gene expression
Lesson period: Second semester
Assessment methods: Esame
Assessment result: voto verbalizzato in trentesimi
Single course
This course cannot be attended as a single course. Please check our list of single courses to find the ones available for enrolment.
Course syllabus and organization
Single session
Responsible
Lesson period
Second semester
If the lessons in presence are not allowed, they will be held online on the Teams platform "Metodologie di Citogenetica".
Course syllabus
- Southern / Northern hybridization methods. Fish.
- Microarrays.
- GWAS.
-PCR and its applications
- Methods based on amplification
- Forensic genetics
- Methods based on NGS
- Structure of the human genome
- Genetic Engineering
- Genome Editing
- Function of the human genome
- Epigenetics Mosaicisms
- Imprinting
- Cellular reprogramming
- Monogenic diseases
- Principles of cancer genetics
- Microarrays.
- GWAS.
-PCR and its applications
- Methods based on amplification
- Forensic genetics
- Methods based on NGS
- Structure of the human genome
- Genetic Engineering
- Genome Editing
- Function of the human genome
- Epigenetics Mosaicisms
- Imprinting
- Cellular reprogramming
- Monogenic diseases
- Principles of cancer genetics
Prerequisites for admission
Basic knowledge of statistics, principles of biophysics, knowledge of cell biology.
Knowledge of basic principles of genetics, molecular biology and biochemistry.
Knowledge of basic principles of genetics, molecular biology and biochemistry.
Teaching methods
The lessons will be part in presence, part online, in the form of oral presentation with slides.
A few lessons will be provided synchronous recorded online, on the Teams platform "Metodologie di Citogenetica", with a small introduction before and a comment session at the end. Such lessons will be available to the students as recorded material on the same platform. All slides will be available as the course progresses on the same site as ppt presentation.
A few lessons will be provided synchronous recorded online, on the Teams platform "Metodologie di Citogenetica", with a small introduction before and a comment session at the end. Such lessons will be available to the students as recorded material on the same platform. All slides will be available as the course progresses on the same site as ppt presentation.
Teaching Resources
"Molecular Human Genetics" T. Strachan, A. Read. Zanichelli
- "HUMAN MOLECULAR GENETICS" J.J.Pasternak Zanichelli
- "INHERITANCE Principles and problems of human genetics." M.R. Cummings EdiSES
- "The Gene" Lewin et al.
- "HUMAN MOLECULAR GENETICS" J.J.Pasternak Zanichelli
- "INHERITANCE Principles and problems of human genetics." M.R. Cummings EdiSES
- "The Gene" Lewin et al.
Assessment methods and Criteria
If allowed, written examination in presence will include open questions. If in presence examinations are not allowed, examinations will be oral online on the Teams platform "Metodologie di Citogenetica".
BIO/18 - GENETICS - University credits: 6
Lessons: 48 hours
Professors:
Dolfini Diletta, Mantovani Roberto
Professor(s)