Biochemistry
A.Y. 2023/2024
Learning objectives
At the end of the course the student will be able to describe the fundamental concepts on how the cell functions, based on its architecture, (macro)molecular components, metabolism, and the role of genetic information. Students will acquire an operative understanding of biochemistry, which will enable them to work within a bioinformatics context, with full comprehension of the key molecular players involved.
Expected learning outcomes
Students will acquire an operative understanding that will enable them to work within a bioinformatics context, with full control and understanding of the acting molecular partners and their interactions.
Lesson period: First semester
Assessment methods: Esame
Assessment result: voto verbalizzato in trentesimi
Single course
This course cannot be attended as a single course. Please check our list of single courses to find the ones available for enrolment.
Course syllabus and organization
Single session
Responsible
Lesson period
First semester
Course syllabus
The following course topics will be covered:
· Introduction to biological macromolecules as the building blocks of living cells: Proteins as polymers of amino acids; nucleotides in relation to energetic considerations; nucleic acids as polymers of nucleotides; polysaccharides and lipids (basic).
· Protein structure: the peptide bond, Ramachandran plot, secondary and higher-level structure organisation. When appropriate, connections will be made to online servers used for the analysis of protein sequences and structures.
· Protein function, with a main focus on enzymes: catalysis, enzyme kinetics (basic), regulation and role of catalysis in metabolism.
· Thermodynamics of metabolism. Biochemical thermodynamics principles; ATP, energy transfer, coupling of endergonic/exergonic reactions.
· Energy metabolism. From glucose to CO2 : Glycolysis, oxidative decarboxylation of pyruvate, the tricarboxylic acid cycle, electron transfer chain and oxidative phosphorylation.
· General biochemistry concepts and methods to analyse the macromolecules of the cell.
· Introduction to biological macromolecules as the building blocks of living cells: Proteins as polymers of amino acids; nucleotides in relation to energetic considerations; nucleic acids as polymers of nucleotides; polysaccharides and lipids (basic).
· Protein structure: the peptide bond, Ramachandran plot, secondary and higher-level structure organisation. When appropriate, connections will be made to online servers used for the analysis of protein sequences and structures.
· Protein function, with a main focus on enzymes: catalysis, enzyme kinetics (basic), regulation and role of catalysis in metabolism.
· Thermodynamics of metabolism. Biochemical thermodynamics principles; ATP, energy transfer, coupling of endergonic/exergonic reactions.
· Energy metabolism. From glucose to CO2 : Glycolysis, oxidative decarboxylation of pyruvate, the tricarboxylic acid cycle, electron transfer chain and oxidative phosphorylation.
· General biochemistry concepts and methods to analyse the macromolecules of the cell.
Prerequisites for admission
By the end of the course the student will be able to describe the structure and function of the four principle (macro)molecular components that comprise a living cell, in particular proteins. Moreover, students will be able to describe the key roles of enzymes in a select few metabolic process that are central in cellular metabolism. Students will acquire the necessary biochemistry competences that will allow them to integrate with, and work in, a bioinformatics context.
Teaching methods
Lessons will consist of frontal lectures, according to the course timetable. Active participation in lessons is strongly encouraged to ensure adequate preparation for the exam.
Teaching Resources
Suggested text books:
- Appling, Antony-Cahill & Mathews: Biochimica, Molecole e Metabolismo (Pearson 2017)
- Nelson D.L. & Cox M.M. - Lehninger's Principles of Biochemistry, Worth Publishers
- Voet D. & Voet, J.G. - Biochemistry, Wiley & Sons.
Additional learning materials (animations, websites and scientific articles) will be indicated and provided by the teacher during the course on the Ariel website.
- Appling, Antony-Cahill & Mathews: Biochimica, Molecole e Metabolismo (Pearson 2017)
- Nelson D.L. & Cox M.M. - Lehninger's Principles of Biochemistry, Worth Publishers
- Voet D. & Voet, J.G. - Biochemistry, Wiley & Sons.
Additional learning materials (animations, websites and scientific articles) will be indicated and provided by the teacher during the course on the Ariel website.
Assessment methods and Criteria
Exams will consist of multiple-choice questions, to assess global understanding of the entire course and open questions, to assess in-depth knowledge of key concepts presented during the course.
Educational website(s)
Professor(s)
Reception:
Upon request
Towerblock 5B, Dep. Biosciences, Via celoria 26, 20133