Chemical and Toxicological Analysis 2
A.Y. 2023/2024
Learning objectives
The main objective of this integrated teaching is to introduce students to the knowledge of the most important instrumental analytical techniques used in the chemical, toxicological and environmental fields, both from a theoretical and practical point of view. For this purpose, the lectures are complemented by classroom exercises and practical laboratories.
Students will acquire theoretical and practical skills related to sampling and processing of samples to prepare them for analytical procedures. They will know the following instrumental analytical techniques: UV-visible analysis, atomic absorption and emission, infrared and Raman spectroscopy, gas chromatography, high performance liquid chromatography, supercritical fluid chromatography, capillary electrophoresis, mass spectrometry and hyphenate techniques, such as GC / MS and HPLC / MS. Depending on the type of sample, they will also be able to choose the correct method to implement the qualitative and quantitative analytical protocol. As far as possible, the student will also have the opportunity to personally verify in the laboratory what has been theoretically acquired.
Students will acquire theoretical and practical skills related to sampling and processing of samples to prepare them for analytical procedures. They will know the following instrumental analytical techniques: UV-visible analysis, atomic absorption and emission, infrared and Raman spectroscopy, gas chromatography, high performance liquid chromatography, supercritical fluid chromatography, capillary electrophoresis, mass spectrometry and hyphenate techniques, such as GC / MS and HPLC / MS. Depending on the type of sample, they will also be able to choose the correct method to implement the qualitative and quantitative analytical protocol. As far as possible, the student will also have the opportunity to personally verify in the laboratory what has been theoretically acquired.
Expected learning outcomes
The student will be able to critically discuss the most common qualitative and quantitative analytical techniques, in particular instrumental methods. He will also acquire the knowledge and skills necessary to: a) solve the problems associated with sampling and handling of samples; b) select suitable analytical techniques to solve a given analytical problem; c) describe an analytical procedure and correctly report an analytical result; d) organize and carry out laboratory activities following high quality and safety standards
Lesson period: year
Assessment methods: Esame
Assessment result: voto verbalizzato in trentesimi
Single course
This course can be attended as a single course.
Course syllabus and organization
Single session
Responsible
Lesson period
year
Course syllabus
The course is focused on the fundamentals of the main instrumental techniques for the environmental analysis of organic molecules. The techniques are those included in the methodologies issued by some official agencies like IRSA, ISO ed EPA. Specifically, the topics discussed in the course are:
- Analysis of compliance according to law limits
- Method validation
- Sampling techniques for environmental matrices like soil, air, water, sediments, and compost
- Purification and extraction of organic molecules from environmental matrices
- Chromatographic techniques both for sample preparation (e.g., SPE, SPME) and instrumental analysis (e.g. GC, HPLC) of environmental organic molecules
- Electrophoretic techniques (e.g., CE, MEKC) for the instrumental analysis of environmental organic molecules
- Qualitative and quantitative analysis of environmental organic molecules through IR, RAMAN, and UV spectroscopy, both in absorbance and emission
- Qualitative and quantitative analysis of environmental organic molecules through mass spectrometry
- Quantitative analysis of environmental organic molecules through immune-enzymatic assays (e.g., ELISA)
The laboratory is focused on teaching the fundamentals of the most popular procedures for the separation, purification, detection and quantitation of environmental organic molecules including:
- Continuous and discontinuous separations and extractions of organic molecules from matrices (e.g., SPE, Soxhlet)
- Instrumental separative techniques (e.g., HPLC, GC)
- Spectroscopy (e.g., UV-Vis)
- Immunoassays (e.g., ELISA)
- Laboratory notebook good practices
- Software data extraction, normalization, and analysis
- Data reporting and presentation
- Analysis of compliance according to law limits
- Method validation
- Sampling techniques for environmental matrices like soil, air, water, sediments, and compost
- Purification and extraction of organic molecules from environmental matrices
- Chromatographic techniques both for sample preparation (e.g., SPE, SPME) and instrumental analysis (e.g. GC, HPLC) of environmental organic molecules
- Electrophoretic techniques (e.g., CE, MEKC) for the instrumental analysis of environmental organic molecules
- Qualitative and quantitative analysis of environmental organic molecules through IR, RAMAN, and UV spectroscopy, both in absorbance and emission
- Qualitative and quantitative analysis of environmental organic molecules through mass spectrometry
- Quantitative analysis of environmental organic molecules through immune-enzymatic assays (e.g., ELISA)
The laboratory is focused on teaching the fundamentals of the most popular procedures for the separation, purification, detection and quantitation of environmental organic molecules including:
- Continuous and discontinuous separations and extractions of organic molecules from matrices (e.g., SPE, Soxhlet)
- Instrumental separative techniques (e.g., HPLC, GC)
- Spectroscopy (e.g., UV-Vis)
- Immunoassays (e.g., ELISA)
- Laboratory notebook good practices
- Software data extraction, normalization, and analysis
- Data reporting and presentation
Prerequisites for admission
Consolidated knowledge of the prerequisite subjects: Organic Chemistry and Chemical Toxicological Analysis 1
Teaching methods
The course has a in-class theory part (7 ECTS - 56 hours) provided as mainly as lectures and partly as flipped classrooms and brakeout rooms. Part of the course will be dedicated to exercices (1 ECTS, 16 hours) and laboratory practice (4 ECTS, 48 hours).
Teaching Resources
Slides and other material published by the teacher on the Ariel webpage of the course.
Ebooks available online at the university library webpage:
Zhang, C. (2007) Fundamentals of environmental sampling and analysis. Hoboken, N.J: Wiley-Interscience.
Dean, J. R. (2014) Environmental trace analysis: techniques and applications. 1st ed. Chichester, West Sussex, United Kingdom: John Wiley & Sons Inc.
Ebooks available online at the university library webpage:
Zhang, C. (2007) Fundamentals of environmental sampling and analysis. Hoboken, N.J: Wiley-Interscience.
Dean, J. R. (2014) Environmental trace analysis: techniques and applications. 1st ed. Chichester, West Sussex, United Kingdom: John Wiley & Sons Inc.
Assessment methods and Criteria
Online anonymous quizzes will be provided during in-class theory part as a self-evaluation tool. The final grades will be determined as the average of a theory test and a laboratory report, weighted according to ECTS (7 for theory, 4 for laboratory). Theory test is a written short answer questionnaire with a maximum duration of two hours, covering any of the topics discussed during the lessons. The test is aimed at evaluating the knowledge of the theory of the topics discussed during the lessons, the ability of interpretation of analytical results and the capability of planning an analytical strategy for the analysis of a given organic molecule.
CHIM/08 - PHARMACEUTICAL CHEMISTRY - University credits: 11
Practicals: 16 hours
Single bench laboratory practical: 48 hours
Lessons: 56 hours
Single bench laboratory practical: 48 hours
Lessons: 56 hours
Professors:
Regazzoni Luca Giovanni, Rizzello Loris
Educational website(s)
Professor(s)
Reception:
on demand, to be scheduled via email
Via L. Mangiagalli 25, first floor, room 1044