Molecular Plant Breeding and Genetics
A.Y. 2023/2024
Learning objectives
The main goal of the course is to supply the students with knowledge about tools and strategies used in breeding programs, combining quantitative genetics and biotechnology to identify the genomic regions containing genes of interest. The effects of domestication and breeding on the genome of crops will be presented to have a wider overview about the challenges that modern breeding has to face.
Expected learning outcomes
After completing this course students will acquire a basic knowledge of the latest molecular breeding approaches. They will become familiar with the technologies available to produce molecular markers and how these are used to construct linkage maps, useful for mapping QTL and plant breeding in general. They will know the pros and cons of alternative strategies available to identify genetic determinants controlling agronomic traits. Students will be able to evaluate the advantages and limitations of different breeding strategies and select the most appropriate one for specific objectives.
Lesson period: Second semester
Assessment methods: Esame
Assessment result: voto verbalizzato in trentesimi
Single course
This course can be attended as a single course.
Course syllabus and organization
Single session
Responsible
Lesson period
Second semester
Course syllabus
The course is composed by 2 integrated modules: 5 ECTS taught by prof. Luca Gianfranceschi and 1 ECTS from by Veronica Gregis.
The following topics will be presented and discussed:
· Challenges of modern plant breeding and how the molecular tools could be used to improve and speed up breeding (Gianfranceschi L.)
· Origin of plant domestication and breeding. Consequences on the available genetic variability (Gianfranceschi L.)
· Overview of plant reproduction systems (Gregis V.)
· RNAi mechanisms related to plant improvement (Gregis V.)
· Epigenetics concepts for plant improvement (Gregis V.)
· Principles of population genetics selection, inbreeding and genetic drift (Gianfranceschi L.)
· Modern breeder's molecular tools: the evolution of molecular markers in the light of new high-throughput sequencing technologies (Gianfranceschi L.)
· Strategies and methods to construct high density linkage maps (Gianfranceschi L.)
· Modern approaches to QTL mapping (Gianfranceschi L.)
· Genome-Wide Association Studies to complement and integrate QTL mapping (Gianfranceschi L.)
· Crossing schemes to obtain breeding populations and discussion of their characteristics (Gianfranceschi L.)
· Experimental design used in breeding programs for the improvement and selection of crop species (Gianfranceschi L.)
The following topics will be presented and discussed:
· Challenges of modern plant breeding and how the molecular tools could be used to improve and speed up breeding (Gianfranceschi L.)
· Origin of plant domestication and breeding. Consequences on the available genetic variability (Gianfranceschi L.)
· Overview of plant reproduction systems (Gregis V.)
· RNAi mechanisms related to plant improvement (Gregis V.)
· Epigenetics concepts for plant improvement (Gregis V.)
· Principles of population genetics selection, inbreeding and genetic drift (Gianfranceschi L.)
· Modern breeder's molecular tools: the evolution of molecular markers in the light of new high-throughput sequencing technologies (Gianfranceschi L.)
· Strategies and methods to construct high density linkage maps (Gianfranceschi L.)
· Modern approaches to QTL mapping (Gianfranceschi L.)
· Genome-Wide Association Studies to complement and integrate QTL mapping (Gianfranceschi L.)
· Crossing schemes to obtain breeding populations and discussion of their characteristics (Gianfranceschi L.)
· Experimental design used in breeding programs for the improvement and selection of crop species (Gianfranceschi L.)
Prerequisites for admission
A basic knowledge of genetics, molecular biology, botany and statistics is highly recommended.
Teaching methods
Teaching mode: Traditional lectures with powerpoint presentations, available to the students after the lecture through Ariel platform. Seminars from scientists working in plant breeding.
Attendance: highly recommended.
Attendance: highly recommended.
Teaching Resources
Copies of the slides projected in the classroom, references to scientific papers and reviews indicated during the course and other study material will be made available through the course website on the ARIEL platform of the University of Milano (http://ariel.ctu.unimi.it).
By no means this material replaces the lectures or a textbook. The material is made available only to registered students of the Degree Course in Molecular Biotechnology and Bioinformatics and should not be distributed to others.
By no means this material replaces the lectures or a textbook. The material is made available only to registered students of the Degree Course in Molecular Biotechnology and Bioinformatics and should not be distributed to others.
Assessment methods and Criteria
There is a single final exam consisting in an oral discussion on topics presented during the course. Usually the discussion starts from the presentation of a scientific paper on topics of the course.
Examples of the examination test will be discussed during classes.
Examples of the examination test will be discussed during classes.
BIO/18 - GENETICS - University credits: 6
Lectures: 48 hours
Professors:
Gianfranceschi Luca Enrico Angelo, Gregis Veronica
Educational website(s)
Professor(s)
Reception:
Upon email request
2nd floor, C building, Dept. of Biosciences