Ree Biocontrol of Pathogens - Innovative, Low Environmental Impact Approaches for the Control of Pathogens (BASIC)
A.Y. 2023/2024
Learning objectives
"Acquire tools and skills on the possibility of using low environmental impact strategies
(Antagonist microorganisms (BCAs) or dsRNA molecules) for the control of plant pathogens and parasites.
Subunit 1: Preparation of control instruments with low environmental impact
a) Isolation and characterization of BCA against different pathogens.
b) Quantification of the BCA / pathogen to be inoculated in subsequent greenhouse tests using digital PCRo
microscope observations
c) Construction of dsRNA molecules for the control of viruses (TAV and vine viruses) and fungi (eg Botrytis
ashy)
d) Quantification of dsRNA by digital PCR
Subunit 2: Tests in a controlled environment and surveys
phenotypic
a) Treatments with BCA and dsRNA on plants experimentally inoculated with the pathogens described above
b) Phenotypic findings; visual analysis, determination of the quantity of chlorophyll a by means of a fluorimeter
c) Statistical analysis
Subunit 3: Determination of the effect on the plant The plants inoculated with the pathogens will come
analyzed with molecular techniques
a) Quantification of inoculated pathogens, BCA and dsRNA by Digital PCR
b) Statistical analysis"
(Antagonist microorganisms (BCAs) or dsRNA molecules) for the control of plant pathogens and parasites.
Subunit 1: Preparation of control instruments with low environmental impact
a) Isolation and characterization of BCA against different pathogens.
b) Quantification of the BCA / pathogen to be inoculated in subsequent greenhouse tests using digital PCRo
microscope observations
c) Construction of dsRNA molecules for the control of viruses (TAV and vine viruses) and fungi (eg Botrytis
ashy)
d) Quantification of dsRNA by digital PCR
Subunit 2: Tests in a controlled environment and surveys
phenotypic
a) Treatments with BCA and dsRNA on plants experimentally inoculated with the pathogens described above
b) Phenotypic findings; visual analysis, determination of the quantity of chlorophyll a by means of a fluorimeter
c) Statistical analysis
Subunit 3: Determination of the effect on the plant The plants inoculated with the pathogens will come
analyzed with molecular techniques
a) Quantification of inoculated pathogens, BCA and dsRNA by Digital PCR
b) Statistical analysis"
Expected learning outcomes
At the end of the course, students will have acquired knowledge and skills on laboratory methods for the study of pathogens using molecular techniques and the control of pathogens using antagonistic microorganisms or dsRNA molecules.
Lesson period: Second semester
Assessment methods: Giudizio di approvazione
Assessment result: superato/non superato
Single course
This course can be attended as a single course.
Course syllabus and organization
Single session
Responsible
Lesson period
Second semester
Course syllabus
Subunit 1: preparation of low environmental impact control tools
a) isolation and characterization of BCA against different pathogenic agents
b) quantification of BCA/pathogens to be inoculated in the following greenhouse trials by use digital PCR
c) synthesis of dsRNA molecules for the control of viruses (TAV and grapevine viruses) or fungi (e.g. Botrytis cinerea)
d) quantification of dsRNA by digital PCR
Subunit 2: trials in controlled environment and phenotype observation
a) treatments with BCA and dsRNA on plants experimentally inoculated with the pathogens previously described
b) phenotype observation: visual examination of symptoms, quantification of chlorophyll through fluorimetric analysis
c) statistical analysis of data
Subunit 3: Detection of treatment effect on the plants
a) quantification of pathogens, BCA, and dsRNA in inoculated plants by digital PCR
b) statistical analysis of data
a) isolation and characterization of BCA against different pathogenic agents
b) quantification of BCA/pathogens to be inoculated in the following greenhouse trials by use digital PCR
c) synthesis of dsRNA molecules for the control of viruses (TAV and grapevine viruses) or fungi (e.g. Botrytis cinerea)
d) quantification of dsRNA by digital PCR
Subunit 2: trials in controlled environment and phenotype observation
a) treatments with BCA and dsRNA on plants experimentally inoculated with the pathogens previously described
b) phenotype observation: visual examination of symptoms, quantification of chlorophyll through fluorimetric analysis
c) statistical analysis of data
Subunit 3: Detection of treatment effect on the plants
a) quantification of pathogens, BCA, and dsRNA in inoculated plants by digital PCR
b) statistical analysis of data
Prerequisites for admission
Knowledge of plant pathology
Teaching methods
Laboratory and screenhouse activities
Teaching Resources
scientific articles
Assessment methods and Criteria
Final Report
AGR/12 - PLANT PATHOLOGY - University credits: 3
Practicals: 24 hours
Laboratories: 16 hours
Lessons: 4 hours
Laboratories: 16 hours
Lessons: 4 hours
Professor(s)
Reception:
On appointment by email (in case of health restrictions on Microsoft Teams)
Second flor - Building 7, via Celoria 2, Milan