Investigation Methodologies in Animal Cell Biology and Histology

The teaching aims to provide the student with basic knowledge of the methods of setting up and maintaining cells and tissues in vitro. The techniques of setting up, maintaining, and storing cell cultures in vitro and their applications, and the preparation techniques for the analysis of tissues used in cytochemistry, histochemistry and immunocytochemistry will be explained. Basic knowledge for the optical visualisation of macromolecules of biological interest will also be provided. Theoretical lectures will be combined with seminars/workshops on examples of the application of these methods to the study of cell biology problems and observation tools for preparations.

By the end of the course, the student will have acquired basic theoretical knowledge related to: - techniques for the preparation, maintenance and in vitro preservation of cell cultures and their applications - techniques for the preparation of histological samples, cytohistochemical and immunohistochemical staining - main methods of in situ observation of biological macromolecules in cells and tissues.