Omics

The aim of the OMICS course is for students to develop knowledge and understanding of genomics and advanced proteomic approaches applied to the qualitative and quantitative study of proteins and peptides in cells and tissues of various origins. Specifically, the Introduction to Genomics module introduces students to the key concepts underlying genome organization. The Proteomics module provides essential information on the main techniques for studying the entire proteome of a cell or tissue, for its qualitative and quantitative characterization, and for analyzing possible changes in response to a stimulus. The broad range of potential applications of this approach will be illustrated, starting with a discussion of published experimental data.

1. Knowledge and Understanding: Regarding the Introduction to Genomics module, by the end of the course, students will demonstrate knowledge of genome size, its organization into chromosomes, the meaning of linkage and linkage disequilibrium, as well as concepts of population genomics (F-statistics).
Regarding the Proteomics module, students will demonstrate knowledge of the principles and applications of the main proteomic methodologies, including: two-dimensional electrophoresis, mass spectrometry, first- and second-order mass analysis, quantitative mass spectrometry, amino-terminal sequencing, amino acid analysis, and the study of complexes.

2. Ability to Apply Knowledge and Understanding: Regarding the Introduction to Genomics module, students will demonstrate knowledge and concepts useful for the use of DNA bead chips for genomic evaluation in cattle or for paternity determination. In the Proteomics module, students must demonstrate the ability to perform and/or interpret a MALDI and ESI mass spectrum, an amino-terminal sequence, and 2D electrophoresis. They must also be able to read and discuss scientific articles related to proteomics and omics applications.
3. Critical thinking and judgment skills: Students must demonstrate the ability to critically argue the information they have learned. This is addressed in the Introduction to Genomics module through specific lessons on case studies and group work. In the Proteomics module, group journal clubs are organized with presentations prepared by students.
4. Ability to communicate what they have learned: Students must demonstrate the ability to express themselves with scientifically appropriate terminology, particularly regarding terminology related to genomics and proteomics. Exercises and group work are intended to foster the ability to express themselves correctly and the ability to engage in scientific discussion with peers.
5. Ability to continue studying independently throughout one's life: the student must demonstrate the ability to use the knowledge acquired to interpret data and develop new experimental projects, drawing on available sources of knowledge and good mental organization.