Veterinary General Pathology and Clinical Biochemistry
A.Y. 2026/2027
Learning objectives
To provide the student with knowledge of the pathogenic mechanisms underlying animal diseases, the main alterations in the functions of organs and systems, and the theoretical and practical bases necessary to choose the most appropriate laboratory analytical methods for clinical biochemistry and clinical veterinary molecular biology analyses.
Expected learning outcomes
1) Knowledge and comprehension skills. On completion of the course, the student will acquire knowledge of the aetiopathogenesis of the main pathological and pathophysiological changes in the organs and systems of animals and theoretical and practical knowledge of the methods of analysis of veterinary clinical chemistry, biochemistry and molecular biology.
2) Ability to apply knowledge and understanding. At the end of the course the student will be able to interpret the most common pathological and pathophysiological alterations and the laboratory data characterising them, as well as to choose the most appropriate methodologies for the measurement of the main biochemical-molecular diagnostic parameters of veterinary interest. The student will know how to mathematically process numerical instrumental results for the calculation and expression of the concentration in the appropriate unit of measurement of disease markers in biological samples and will have acquired preliminary skills to assess the reliability and diagnostic value of the analytical result.
3) Critical skills and judgement. The student will develop critical skills and autonomy of judgement by tackling problems of diagnostic-clinical pathology and laboratory methodologies during practical exercises in the biochemistry, microscopy and computer laboratories. The ability to integrate notions received in the various modules of the course will also be developed through the proposal of practical clinical cases that students in small groups must evaluate and solve independently with regard to aspects of pathology, immunopathology and clinical biochemistry.
4) Ability to communicate what has been learned. The ability to communicate the knowledge of pathology and clinical biochemistry using appropriate, up-to-date terminology consistent with the professionalism required for a veterinarian will be acquired and verified during classroom discussions, practical exercises in the laboratories and during meetings in which the aspects of pathology and clinical biochemistry related to the practical clinical cases that the students have assessed independently are discussed collegially. The ability to adequately communicate the following is also verified in the examination by assessing the answers to open-ended questions.
5) Ability to pursue study autonomously throughout life. The knowledge in terms of notions and methodologies and the reasoning skills acquired during teaching and the need to deepen autonomously, also by means of computer tools (querying databases, image research, etc.), some concepts during the elaboration of cases and images provided during the exercises, will enable the student to continue independently the study, deepening and continuous updating of his knowledge in the field of pathological and pathophysiological alterations of organs and apparatuses and methods of clinical biochemical analysis both during his university career and later as a veterinary surgeon.
2) Ability to apply knowledge and understanding. At the end of the course the student will be able to interpret the most common pathological and pathophysiological alterations and the laboratory data characterising them, as well as to choose the most appropriate methodologies for the measurement of the main biochemical-molecular diagnostic parameters of veterinary interest. The student will know how to mathematically process numerical instrumental results for the calculation and expression of the concentration in the appropriate unit of measurement of disease markers in biological samples and will have acquired preliminary skills to assess the reliability and diagnostic value of the analytical result.
3) Critical skills and judgement. The student will develop critical skills and autonomy of judgement by tackling problems of diagnostic-clinical pathology and laboratory methodologies during practical exercises in the biochemistry, microscopy and computer laboratories. The ability to integrate notions received in the various modules of the course will also be developed through the proposal of practical clinical cases that students in small groups must evaluate and solve independently with regard to aspects of pathology, immunopathology and clinical biochemistry.
4) Ability to communicate what has been learned. The ability to communicate the knowledge of pathology and clinical biochemistry using appropriate, up-to-date terminology consistent with the professionalism required for a veterinarian will be acquired and verified during classroom discussions, practical exercises in the laboratories and during meetings in which the aspects of pathology and clinical biochemistry related to the practical clinical cases that the students have assessed independently are discussed collegially. The ability to adequately communicate the following is also verified in the examination by assessing the answers to open-ended questions.
5) Ability to pursue study autonomously throughout life. The knowledge in terms of notions and methodologies and the reasoning skills acquired during teaching and the need to deepen autonomously, also by means of computer tools (querying databases, image research, etc.), some concepts during the elaboration of cases and images provided during the exercises, will enable the student to continue independently the study, deepening and continuous updating of his knowledge in the field of pathological and pathophysiological alterations of organs and apparatuses and methods of clinical biochemical analysis both during his university career and later as a veterinary surgeon.
Lesson period: Second semester
Assessment methods: Esame
Assessment result: voto verbalizzato in trentesimi
Single course
This course cannot be attended as a single course. Please check our list of single courses to find the ones available for enrolment.
Course syllabus and organization
Single session
Responsible
Lesson period
Second semester
Prerequisites for admission
In order to be able to take the General Veterinary Pathology and Clinical Biochemistry examination, attendance at 70% of the courses and tutorials of all three modules and successful completion of the (compulsory propaedeuticity) examinations are required:
- Veterinary bacteriology, virology and immunology
- Systematic and comparative veterinary anatomy 3
- Physiology and endocrinology of domestic animals
- Physiology and ethology of domestic animals
Passing the General Veterinary Pathology and Clinical Biochemistry examination is a prerequisite for the examinations of:
- Veterinary Pathological Anatomy 1
- Veterinary Pharmacology and Toxicology
- Infectious and parasitic diseases of pets and horses
- Infectious and parasitic diseases of farm animals and herd health management
- Veterinary bacteriology, virology and immunology
- Systematic and comparative veterinary anatomy 3
- Physiology and endocrinology of domestic animals
- Physiology and ethology of domestic animals
Passing the General Veterinary Pathology and Clinical Biochemistry examination is a prerequisite for the examinations of:
- Veterinary Pathological Anatomy 1
- Veterinary Pharmacology and Toxicology
- Infectious and parasitic diseases of pets and horses
- Infectious and parasitic diseases of farm animals and herd health management
Assessment methods and Criteria
There are 8 exam session and are held in January, February, April, June, July, September, October and November/December. Two extra exam sessions only for students enrolled in supplementary years take place on request in March and May.
In order to sit the exam, it is essential to register for the exam via the University's SIFA online service (N.B.: registration closes 3 days before the exam date) and the compulsory propaedeutic exams must have been passed.
The examination takes place in a single session for the three modules of the integrated course and consists of a written exam of a maximum duration of 120 minutes. The student who obtains ≥ 18 points in each module passes the written examination. A student who obtains 16 or 17 points in a module is obliged to take an oral examination. A student who scores 16 points in two or more modules is failed.
The oral examination to make up the failure in a module of the written examination lasts 10-15 minutes and covers specific topics from the syllabus of the module related to the failed section. The ability to correct the errors made in the written test and whether the failure is due to a real criticality in the knowledge and understanding of the syllabus topics of that module will be assessed. If the oral test is not passed, the student must retake the examination of the entire integrated course. Rubrics with the evaluation criteria for the oral examination can be found on the course's Ariel website.
Students who scored ≥ 18 in the three modules of the written examination and wish to increase their score in one or more modules may apply to take an oral examination on a voluntary basis. The optional 10-15 minutes oral examination covers specific topics from the module programme relating to the section with the grade the student wishes to improve. The test includes a critical discussion of the written result and in-depth questions on specific topics of the syllabus. Rubrics with the evaluation criteria for the oral examination can be found on the course's Ariel website.
The mark expressed in thirtieths is calculated as a weighted average over the CFUs of the marks obtained in the sections of the written paper relating to the three modules. The marks for the individual modules are calculated as follows:
MODULE OF GENERAL VETERINARY PATHOLOGY (maximum 32 points)
The written test consists of two sections. (A) Four multiple-choice and/or single-choice open-ended questions on the general aetiology or pathogenesis of cell damage, adaptation mechanisms or intra- and extracellular degeneration, inflammation, and tumours; 2 points will be awarded for each correct answer; 1 point for each partially correct answer; 0 points for each incorrect or missing answer. (B) Four questions with three short open-ended answers on the general aetiology or pathogenesis of cellular damage, adaptation mechanisms or intra- and extracellular degeneration, inflammation, and tumours; 2 marks will be awarded for each correct answer; 1 mark for each partially correct answer; 0 marks for each incorrect or missing answer.
PATHOPHYSIOLOGY AND IMMUNOPATHOLOGY MODULE (maximum 32 points)
The written test consists of two sections. (A) Four questions with multiple-choice and/or unequivocal open answers on blood or plasma protein diseases, endocrine diseases, circulatory disorders, organ function tests or fever, and immunopathology; 2 points will be awarded for each correct answer; 1 point for each partially correct answer; 0 points for each incorrect or no answer. (B) Four questions involving three short open-ended answers on blood or plasma protein diseases, endocrine diseases, circulatory disorders, organ function tests or fever, and immunopathology; 2 marks will be awarded for each correct answer; 1 mark for each partially correct answer; 0 marks for each incorrect or not given answer.
CLINICAL BIOCHEMISTRY MODULE (maximum 32 points)
The written test consists of three sections. (A) Sixteen multiple-choice and/or single-choice open-answer questions in which the student must demonstrate knowledge and ability to understand the theoretical principles and practical aspects of the main biochemistry and molecular biology analysis methods used in veterinary diagnostic laboratories: 0.75 points for each correct answer; 0.4 points for each partially correct answer; 0.0 points for each incorrect answer. (B) Two articulated open-ended questions in which the student must demonstrate knowledge of the theoretical principles and practical aspects of the main methods of biochemistry and molecular biology analysis used in veterinary diagnostic laboratories. The ability to convert an instrumental result into a biochemical diagnostic parameter will also be assessed: 10 marks maximum for the first question and 4 marks maximum for the second question. (C) Five calculation exercises on transformations between concentration units, preparation of solutions, calculation of enzyme activity, simple questions on the transformation of the instrumental reading of an analysis into the concentration of the analyte: 1.2 points for each correct answer, 0.6 points for each partially correct answer, 0.0 points for each incorrect answer.
In order to sit the exam, it is essential to register for the exam via the University's SIFA online service (N.B.: registration closes 3 days before the exam date) and the compulsory propaedeutic exams must have been passed.
The examination takes place in a single session for the three modules of the integrated course and consists of a written exam of a maximum duration of 120 minutes. The student who obtains ≥ 18 points in each module passes the written examination. A student who obtains 16 or 17 points in a module is obliged to take an oral examination. A student who scores 16 points in two or more modules is failed.
The oral examination to make up the failure in a module of the written examination lasts 10-15 minutes and covers specific topics from the syllabus of the module related to the failed section. The ability to correct the errors made in the written test and whether the failure is due to a real criticality in the knowledge and understanding of the syllabus topics of that module will be assessed. If the oral test is not passed, the student must retake the examination of the entire integrated course. Rubrics with the evaluation criteria for the oral examination can be found on the course's Ariel website.
Students who scored ≥ 18 in the three modules of the written examination and wish to increase their score in one or more modules may apply to take an oral examination on a voluntary basis. The optional 10-15 minutes oral examination covers specific topics from the module programme relating to the section with the grade the student wishes to improve. The test includes a critical discussion of the written result and in-depth questions on specific topics of the syllabus. Rubrics with the evaluation criteria for the oral examination can be found on the course's Ariel website.
The mark expressed in thirtieths is calculated as a weighted average over the CFUs of the marks obtained in the sections of the written paper relating to the three modules. The marks for the individual modules are calculated as follows:
MODULE OF GENERAL VETERINARY PATHOLOGY (maximum 32 points)
The written test consists of two sections. (A) Four multiple-choice and/or single-choice open-ended questions on the general aetiology or pathogenesis of cell damage, adaptation mechanisms or intra- and extracellular degeneration, inflammation, and tumours; 2 points will be awarded for each correct answer; 1 point for each partially correct answer; 0 points for each incorrect or missing answer. (B) Four questions with three short open-ended answers on the general aetiology or pathogenesis of cellular damage, adaptation mechanisms or intra- and extracellular degeneration, inflammation, and tumours; 2 marks will be awarded for each correct answer; 1 mark for each partially correct answer; 0 marks for each incorrect or missing answer.
PATHOPHYSIOLOGY AND IMMUNOPATHOLOGY MODULE (maximum 32 points)
The written test consists of two sections. (A) Four questions with multiple-choice and/or unequivocal open answers on blood or plasma protein diseases, endocrine diseases, circulatory disorders, organ function tests or fever, and immunopathology; 2 points will be awarded for each correct answer; 1 point for each partially correct answer; 0 points for each incorrect or no answer. (B) Four questions involving three short open-ended answers on blood or plasma protein diseases, endocrine diseases, circulatory disorders, organ function tests or fever, and immunopathology; 2 marks will be awarded for each correct answer; 1 mark for each partially correct answer; 0 marks for each incorrect or not given answer.
CLINICAL BIOCHEMISTRY MODULE (maximum 32 points)
The written test consists of three sections. (A) Sixteen multiple-choice and/or single-choice open-answer questions in which the student must demonstrate knowledge and ability to understand the theoretical principles and practical aspects of the main biochemistry and molecular biology analysis methods used in veterinary diagnostic laboratories: 0.75 points for each correct answer; 0.4 points for each partially correct answer; 0.0 points for each incorrect answer. (B) Two articulated open-ended questions in which the student must demonstrate knowledge of the theoretical principles and practical aspects of the main methods of biochemistry and molecular biology analysis used in veterinary diagnostic laboratories. The ability to convert an instrumental result into a biochemical diagnostic parameter will also be assessed: 10 marks maximum for the first question and 4 marks maximum for the second question. (C) Five calculation exercises on transformations between concentration units, preparation of solutions, calculation of enzyme activity, simple questions on the transformation of the instrumental reading of an analysis into the concentration of the analyte: 1.2 points for each correct answer, 0.6 points for each partially correct answer, 0.0 points for each incorrect answer.
General Veterinary pathology
Course syllabus
- Introduction (Introduction to the course - concept of homeostasis and disease). 1 hour.
- General aetiology (Physical causes of disease - radiation and burns; Chemical causes of disease; Living agents as a cause of disease; Genetic-inherited causes). 4 hours.
- Cell and tissue death (Necrosis, Apoptosis). 2 hours.
- Adaptive mechanisms (Hypertrophy, Hypotrophy, Metaplasia). 2 hours.
- Cellular degeneration (Turbid swelling, Hydropic degeneration, Vacuolar degeneration, Mucosal degeneration, Fatty degeneration or steatosis, Cholesterinosis - atheromas). 4 hours.
- Extracellular degeneration (Jalin degeneration, Fibrinoid degeneration or necrosis, Mucous connective degeneration, Amyloid degeneration or amyloidosis). 3 hours.
- The acute inflammatory process or angioflogosis (Phases of angioflogosis, Vasomotor changes during angioflogosis, Cell activation, diapedesis and phagocytosis, Chemical mediators of the inflammatory process, Classification of exudates, The acute phase reaction). 6 hours.
- The chronic inflammatory process or histophlogosis (interstitial histophlogosis, granulomatous histophlogosis). 2 hours.
- Wound repair (Mechanisms of healing by first and second intention). 2 hours.
- Tumours (Definitions and nomenclature, Morphological, biochemical and metabolic neoplastic atypicalities, Mechanisms of oncogenesis, Molecular oncogenesis, The stages of neoplastic development, Metastatisation, Cachexia and paraneoplastic syndromes, Immunity and tumours). 6 hours.
- General aetiology (Physical causes of disease - radiation and burns; Chemical causes of disease; Living agents as a cause of disease; Genetic-inherited causes). 4 hours.
- Cell and tissue death (Necrosis, Apoptosis). 2 hours.
- Adaptive mechanisms (Hypertrophy, Hypotrophy, Metaplasia). 2 hours.
- Cellular degeneration (Turbid swelling, Hydropic degeneration, Vacuolar degeneration, Mucosal degeneration, Fatty degeneration or steatosis, Cholesterinosis - atheromas). 4 hours.
- Extracellular degeneration (Jalin degeneration, Fibrinoid degeneration or necrosis, Mucous connective degeneration, Amyloid degeneration or amyloidosis). 3 hours.
- The acute inflammatory process or angioflogosis (Phases of angioflogosis, Vasomotor changes during angioflogosis, Cell activation, diapedesis and phagocytosis, Chemical mediators of the inflammatory process, Classification of exudates, The acute phase reaction). 6 hours.
- The chronic inflammatory process or histophlogosis (interstitial histophlogosis, granulomatous histophlogosis). 2 hours.
- Wound repair (Mechanisms of healing by first and second intention). 2 hours.
- Tumours (Definitions and nomenclature, Morphological, biochemical and metabolic neoplastic atypicalities, Mechanisms of oncogenesis, Molecular oncogenesis, The stages of neoplastic development, Metastatisation, Cachexia and paraneoplastic syndromes, Immunity and tumours). 6 hours.
Teaching methods
Lectures (32 hours) by means of slides with diagrams, text and explanatory videos. During the lectures there are interactive discussions of points of interest and at the end of each group of topics a self-assessment test using computer tool. EAEVE teaching category: 32 hours A.
Teaching Resources
- Notes or 'on-line' version on the Ariel website
- McGAVIN and ZACHARY - General Veterinary Pathology - Elsevier Masson 2008
- PONTIERI - General Pathology -1 and 2 (2 for pathophysiology) - Piccin. Latest edition
- ROBBINS - Pathologic basis of the disease - 6th (2000)/7th (2004) ed. Saunders
The following day one competences (DOCs) provided by EAEVE will be delivered in this module: 5, 10, 14, 25.
- McGAVIN and ZACHARY - General Veterinary Pathology - Elsevier Masson 2008
- PONTIERI - General Pathology -1 and 2 (2 for pathophysiology) - Piccin. Latest edition
- ROBBINS - Pathologic basis of the disease - 6th (2000)/7th (2004) ed. Saunders
The following day one competences (DOCs) provided by EAEVE will be delivered in this module: 5, 10, 14, 25.
Pathophysiology and immunopathology
Course syllabus
LESSONS
- Blood pathophysiology (Modifications of blood volume, Anaemia, Polycythaemia, Leukocytopenia, Thrombocytopenia, Alterations of plasma proteins). 5 hours.
- Pathophysiology of the endocrine system (Hypo- and hyperfunctional syndromes of the pituitary gland, thyroid, adrenal glans, General Adaptation Syndrome. (stress), endocrine pancreas: diabetes mellitus). 5 hours.
- Pathophysiology of metabolism (Bovine ketosis and toxaemia gravidarum of small ruminants; Neonatal hypoglycaemia of the piglet; Puerperal collapse). 4 hours.
- Circulatory disorders (Hyperaemia, Oedema, Ischaemia, Infarction, Thrombosis, Embolism, Shock) 2 hours
- Pathophysiology of thermoregulation (non-febrile hyperthermia, fever). 1 hour.
- Pathological pigmentations: jaundice. 1 hour.
- Functionality tests (Interpretative principles, Liver function tests). 1 hour.
- Immunopathology (Hypersensitivity reactions (Type I, II, III, IV), Autoimmune diseases, Immunodeficiencies. 5 hours.
EXERCISES
- Exercise 1: Methods, analytical principles and pre-analytical errors (single group in the classroom): students are provided in advance, via the Ariel website, with laboratory data produced with the different methods or falsified by pre-analytical errors and in the classroom, after a short presentation on the methods they will use in the laboratory (exercise 2), the cases presented as well as the advantages and disadvantages of the different methods are discussed through questions on the wooclap platform. 2 hours
- Exercise 2: Handling and analysis of blood samples (groups of up to 20 students in the laboratory): Cell counts, Individual blood smear execution, Automated biochemical determinations. 2 hours.
- Exercise 3: Blood smear analysis and cell recognition (single group in the classroom): Students are provided with a file of images of the main normal and pathological cells found in blood on Ariel. Questions are presented in the classroom for each image on the wooclap platform, and the answers are commented on collectively. 2 hours
- Exercise 4: Reading of non-pathological blood smears under the microscope (microscope room with single microscope, groups of max. 30 students): method of reading normal smears of different animal species, Performing the leukocyte formula: 4 hours
- Exercise 5: Reading pathological blood smears under the microscope (microscope room with single microscope, groups of max. 30 students): Recognition of basic erythrogram and leucogram alterations. 8 hours.
- Exercise 6: Principles of interpretation (single group in the classroom), clinical cases derived from the diagnostic routine of the university veterinary hospital are presented in the classroom and through questions on the wooclap platform, the participants are asked to identify the main pathophysiological changes described in the course and displayed in the previous exercises: 2 hours.
- Blood pathophysiology (Modifications of blood volume, Anaemia, Polycythaemia, Leukocytopenia, Thrombocytopenia, Alterations of plasma proteins). 5 hours.
- Pathophysiology of the endocrine system (Hypo- and hyperfunctional syndromes of the pituitary gland, thyroid, adrenal glans, General Adaptation Syndrome. (stress), endocrine pancreas: diabetes mellitus). 5 hours.
- Pathophysiology of metabolism (Bovine ketosis and toxaemia gravidarum of small ruminants; Neonatal hypoglycaemia of the piglet; Puerperal collapse). 4 hours.
- Circulatory disorders (Hyperaemia, Oedema, Ischaemia, Infarction, Thrombosis, Embolism, Shock) 2 hours
- Pathophysiology of thermoregulation (non-febrile hyperthermia, fever). 1 hour.
- Pathological pigmentations: jaundice. 1 hour.
- Functionality tests (Interpretative principles, Liver function tests). 1 hour.
- Immunopathology (Hypersensitivity reactions (Type I, II, III, IV), Autoimmune diseases, Immunodeficiencies. 5 hours.
EXERCISES
- Exercise 1: Methods, analytical principles and pre-analytical errors (single group in the classroom): students are provided in advance, via the Ariel website, with laboratory data produced with the different methods or falsified by pre-analytical errors and in the classroom, after a short presentation on the methods they will use in the laboratory (exercise 2), the cases presented as well as the advantages and disadvantages of the different methods are discussed through questions on the wooclap platform. 2 hours
- Exercise 2: Handling and analysis of blood samples (groups of up to 20 students in the laboratory): Cell counts, Individual blood smear execution, Automated biochemical determinations. 2 hours.
- Exercise 3: Blood smear analysis and cell recognition (single group in the classroom): Students are provided with a file of images of the main normal and pathological cells found in blood on Ariel. Questions are presented in the classroom for each image on the wooclap platform, and the answers are commented on collectively. 2 hours
- Exercise 4: Reading of non-pathological blood smears under the microscope (microscope room with single microscope, groups of max. 30 students): method of reading normal smears of different animal species, Performing the leukocyte formula: 4 hours
- Exercise 5: Reading pathological blood smears under the microscope (microscope room with single microscope, groups of max. 30 students): Recognition of basic erythrogram and leucogram alterations. 8 hours.
- Exercise 6: Principles of interpretation (single group in the classroom), clinical cases derived from the diagnostic routine of the university veterinary hospital are presented in the classroom and through questions on the wooclap platform, the participants are asked to identify the main pathophysiological changes described in the course and displayed in the previous exercises: 2 hours.
Teaching methods
Lectures (24 hours) by means of slides with diagrams, text and explanatory videos. During the lectures there will be interactive discussions of points of interest and at the end of each group of topics a self-assessment test using IT tools. EAEVE teaching category: 24 hours A.
Practical exercises (16 hours) will be carried out as indicated in the syllabus at the biological or microscopy laboratories of the Lodi teaching centre. EAEVE teaching category: 6 hours C, 10 hours D.
Practical exercises (16 hours) will be carried out as indicated in the syllabus at the biological or microscopy laboratories of the Lodi teaching centre. EAEVE teaching category: 6 hours C, 10 hours D.
Teaching Resources
- Lecture notes and teaching materials uploaded by the lecturer on the university's ARIEL portal dedicated to the course.
- PONTIERI - General Pathology -1 and 2 (2 for pathophysiology) - Piccin. Latest edition
- TIZARD - Veterinary Immunolgy - An Introduction, 6th ed. Saunders
For specific interests (blood diseases and endocrinology):
- WEISS and WARDROP- Schalm's Veterinary Hematology - 6th ed. Lippincott, Williams & Wilkins
- KANEKO - Clinical Biochemistry of domestic animals - 6th ed.
- STOCKHAM & SCOTT - Fundamentals of Veterinary Clinical Pathology - 2nd ed.
- PALTRINIERI GIORDANO, BERTAZZOLO - Clinical Pathology of Dogs and Cats. Practical approach to laboratory diagnostics.
The following EAEVE day one competences (DOCs) will be delivered in this module: 4,5,7,10,11,14,16,22,23,24,25,29
- PONTIERI - General Pathology -1 and 2 (2 for pathophysiology) - Piccin. Latest edition
- TIZARD - Veterinary Immunolgy - An Introduction, 6th ed. Saunders
For specific interests (blood diseases and endocrinology):
- WEISS and WARDROP- Schalm's Veterinary Hematology - 6th ed. Lippincott, Williams & Wilkins
- KANEKO - Clinical Biochemistry of domestic animals - 6th ed.
- STOCKHAM & SCOTT - Fundamentals of Veterinary Clinical Pathology - 2nd ed.
- PALTRINIERI GIORDANO, BERTAZZOLO - Clinical Pathology of Dogs and Cats. Practical approach to laboratory diagnostics.
The following EAEVE day one competences (DOCs) will be delivered in this module: 4,5,7,10,11,14,16,22,23,24,25,29
Clinical biochemistry
Course syllabus
LECTURES
- Units of measurement in clinical biochemistry. SI and conventional units in the expression of concentrations (weight/volume, molarity, ppm and ppb, percentage). (2 hours).
- Radiant energy. Absorbance, transmittance, spectrophotometry, fluorometry, luminometry, refractometry, reflectance spectroscopy, atomic absorption. (1 hour).
- From instrumental reading to analyte concentration. (2 hours).
- Clinical chemistry methods. (1 hour).
- Enzymatic methods for the assay of metabolites of clinical biochemical interest in biological fluids. (1 hour).
- Enzymatic methods for the assay of enzymes of clinical biochemical interest in biological fluids. (2 hours).
- Immunochemical methods for antibody and antigen assays: direct tests and tests with competitive and non-competitive labelled reagents (e.g. ELISA) (2 hours)
- Electrophoretic methods. General principles, net charge electrophoresis, SDS-electrophoresis. Interpretation of electropherogram. (2 hours).
- Electrochemical methods. Measurements in potentiometry with ion-selective electrodes and measurements in amperometry. (1 hour).
- Atomic photometry. Atomic absorption and emission. (1 hour).
- Molecular biology techniques. Analysis of PCR products (1 hours).
- Molecular biology techniques. PCR, analysis of PCR products, sequencing. (2 hours).
- Factors influencing laboratory's test results. Pre-analytical variability. Analytical variability: precision, accuracy, sensitivity, limit of determinability, specificity, cross-reactivity, species-specificity. Objectives for analytical errors. (4 hours).
- Interpretation of laboratory analysis for diagnostic purposes. Biological variability. Reference values and ranges. Pathognomonic and non-pathognomonic testing. Concepts of diagnostic sensitivity and specificity, prevalence, calculation of negative predictive values and positive predictive values of diagnostic tests. (4 hours).
PRACTICAL TRAINING
Exercises on conversions between quantities and units used in clinical biochemistry and calculations for the preparation of solutions at specific concentrations. The lecturer proposes calculation exercises that students solve individually or in small groups, with the possibility of requesting support from the instructor. Each exercise is followed by a collective review and correction. (12 hours, classroom).
- Mathematical processing, graphical representation, and critical evaluation of laboratory results for biomolecules in biological samples. Students construct and complete Excel spreadsheets using instrumental numerical data obtained from clinical chemistry assays, sandwich and competitive ELISAs, and protein and DNA electrophoresis, in order to derive molecular characteristics and the concentration of the measured analyte from the instrumental output. Students work individually at computer workstations equipped with all materials required for the exercise. They may request support from the instructor and/or discuss with peers. At the end of the session, each student submits their completed assignment to the instructor by email. (4 hours, computer rooms).
- Units of measurement in clinical biochemistry. SI and conventional units in the expression of concentrations (weight/volume, molarity, ppm and ppb, percentage). (2 hours).
- Radiant energy. Absorbance, transmittance, spectrophotometry, fluorometry, luminometry, refractometry, reflectance spectroscopy, atomic absorption. (1 hour).
- From instrumental reading to analyte concentration. (2 hours).
- Clinical chemistry methods. (1 hour).
- Enzymatic methods for the assay of metabolites of clinical biochemical interest in biological fluids. (1 hour).
- Enzymatic methods for the assay of enzymes of clinical biochemical interest in biological fluids. (2 hours).
- Immunochemical methods for antibody and antigen assays: direct tests and tests with competitive and non-competitive labelled reagents (e.g. ELISA) (2 hours)
- Electrophoretic methods. General principles, net charge electrophoresis, SDS-electrophoresis. Interpretation of electropherogram. (2 hours).
- Electrochemical methods. Measurements in potentiometry with ion-selective electrodes and measurements in amperometry. (1 hour).
- Atomic photometry. Atomic absorption and emission. (1 hour).
- Molecular biology techniques. Analysis of PCR products (1 hours).
- Molecular biology techniques. PCR, analysis of PCR products, sequencing. (2 hours).
- Factors influencing laboratory's test results. Pre-analytical variability. Analytical variability: precision, accuracy, sensitivity, limit of determinability, specificity, cross-reactivity, species-specificity. Objectives for analytical errors. (4 hours).
- Interpretation of laboratory analysis for diagnostic purposes. Biological variability. Reference values and ranges. Pathognomonic and non-pathognomonic testing. Concepts of diagnostic sensitivity and specificity, prevalence, calculation of negative predictive values and positive predictive values of diagnostic tests. (4 hours).
PRACTICAL TRAINING
Exercises on conversions between quantities and units used in clinical biochemistry and calculations for the preparation of solutions at specific concentrations. The lecturer proposes calculation exercises that students solve individually or in small groups, with the possibility of requesting support from the instructor. Each exercise is followed by a collective review and correction. (12 hours, classroom).
- Mathematical processing, graphical representation, and critical evaluation of laboratory results for biomolecules in biological samples. Students construct and complete Excel spreadsheets using instrumental numerical data obtained from clinical chemistry assays, sandwich and competitive ELISAs, and protein and DNA electrophoresis, in order to derive molecular characteristics and the concentration of the measured analyte from the instrumental output. Students work individually at computer workstations equipped with all materials required for the exercise. They may request support from the instructor and/or discuss with peers. At the end of the session, each student submits their completed assignment to the instructor by email. (4 hours, computer rooms).
Teaching methods
- Lectures (24 hours). Formal classroom instruction supported by slide presentations. The lectures introduce the principles and applications of major biochemical and clinical molecular biology methodologies used in veterinary clinical biochemistry laboratories. EAEVE teaching category: 24 hours A.
- Practical classes (16 hours). Classroom and computer based practical sessions, with students working individually and in small groups. Activities include numerical exercises and data processing aimed at converting instrumental measurements into the concentration of biochemical diagnostic parameters. EAEVE teaching category: 10 hours B, 6 hours D.
- Practical classes (16 hours). Classroom and computer based practical sessions, with students working individually and in small groups. Activities include numerical exercises and data processing aimed at converting instrumental measurements into the concentration of biochemical diagnostic parameters. EAEVE teaching category: 10 hours B, 6 hours D.
Teaching Resources
- Lecture notes and material distributed and published by the lecturer on the university's ARIEL website dedicated to the course.
- Textbooks:
Clinical Biochemistry, 2nd edition (2000), Luigi Spandrio, Sorbonne Editions. Part One (General Clinical Biochemistry)
- For more information:
Biochemical Methodology - Keith Wilson and John Walker, 5th edition - Italian Eds Pilone and Pollegioni, Raffaello Cortina Editore (2006).
Clinical biochemistry of domestic animals, 6th edition (2008), Kaneko JJ, Academic press.
- Textbooks:
Clinical Biochemistry, 2nd edition (2000), Luigi Spandrio, Sorbonne Editions. Part One (General Clinical Biochemistry)
- For more information:
Biochemical Methodology - Keith Wilson and John Walker, 5th edition - Italian Eds Pilone and Pollegioni, Raffaello Cortina Editore (2006).
Clinical biochemistry of domestic animals, 6th edition (2008), Kaneko JJ, Academic press.
Modules or teaching units
Clinical biochemistry
BIO/12 - CLINICAL BIOCHEMISTRY AND MOLECULAR BIOLOGY - University credits: 4
Exercises: 16 hours
Lessons: 24 hours
Lessons: 24 hours
Professor:
Borromeo Vitaliano
Shifts:
Professor:
Borromeo Vitaliano
1 - turno unico per tutti gli studenti
Professor:
Borromeo Vitaliano2 - turno ripetuto per un gruppo di studenti
Professor:
Borromeo Vitaliano3 - turno ripetuto per un gruppo di studenti
Professor:
Borromeo Vitaliano
General Veterinary pathology
VET/03 - VETERINARY PATHOLOGY - University credits: 4
Lessons: 32 hours
Professor:
Paltrinieri Saverio
Pathophysiology and immunopathology
VET/03 - VETERINARY PATHOLOGY - University credits: 4
Exercises: 16 hours
Lessons: 24 hours
Lessons: 24 hours
Professor:
Paltrinieri Saverio
Shifts:
Professor:
Paltrinieri Saverio
1 - turno unico per tutti gli studenti
Professor:
Paltrinieri Saverio2 - turno ripetuto per un gruppo di studenti
Professor:
Paltrinieri Saverio3 - turno ripetuto per un gruppo di studenti
Professor:
Paltrinieri Saverio4 - turno ripetuto per un gruppo di studenti
Professor:
Paltrinieri Saverio5 - turno ripetuto per un gruppo di studenti
Professor:
Paltrinieri Saverio6 - turno ripetuto per un gruppo di studenti
Professor:
Paltrinieri Saverio7 - turno ripetuto per un gruppo di studenti
Professor:
Paltrinieri Saverio8 - turno ripetuto per un gruppo di studenti
Professor:
Paltrinieri SaverioProfessor(s)
Reception:
Every day, by appointment
Lodi or online on Teams