In Vitro Model Technologies

The Course In vitro Model Technologies aims to provide information on innovative methodologies in the field of conveying cells and tissues, newly developed in vitro culture platforms for in vitro modeling and in vitro toxicology to evaluate the absorption, bioavailability, metabolism and toxicity of substances through a practical approach.

At the end of the course, students will be able to:
Knowledge and understanding
- describe the principles, potential and limitations of the main in vitro models used in toxicology and physiology;
- explain endocrine, paracrine and cell-signalling functions in 3D systems, particularly those based on primary cells;
- explain the principles of controlled delivery and release of cells and tissues and the role of the extracellular matrix;
- describe the main next-generation culture platforms for in vitro tissue reconstruction and maintenance.
Applying knowledge and understanding
- select an appropriate in vitro model for a specific experimental question;
- apply and interpret cytotoxicity, permeability, metabolism and cellular-response assays;
- compare 2D cultures, 3D systems, co-cultures, organoids and microfluidic platforms in terms of suitability, reproducibility and biological relevance.
Making judgements
- critically analyse experimental data and methodological limitations, including sample quality, controls, variability and transferability of results.
Communication skills
- clearly present and discuss results, protocols and experimental choices using appropriate scientific terminology.
Learning skills
- independently consult scientific literature, guidelines and teaching materials to update their knowledge of in vitromodels.