Microbiologia molecolare

Didattica frontale
Aspetti generali dell'interazione microrganismo-ospite
Aspetti molecolari del sistema immunitario dell'ospite
I recettori: le molecole che riconoscono l'antigene e attivano la trasduzione del segnale
Interazioni batteri-ospite
Interazioni virus-ospite
Esercitazioni
I recettori per i patogeni: isolamento e caratterizzazione molecolare

Unità didattica: Basi molecolari della patogenicità microbica:
Parte teorica:
Introduzione al corso
Meccanismi genetici nella patogenicità
I fattori di virulenza: tecniche per l' identificazione e loro caratteristiche
Virulenza e sua regolazione: sistemi di secrezione e quorum sensing
Strategie molecolari per l'evasione dal sistema immunitario dell'ospite
Gli antibiotici e la resistenza
Microorganismi patogeni di interesse veterinario
Nuove strategie nel controllo della patogenicità: il microbioma e la sua modulazione

ESERCITAZIONI:
metodiche bioinformatiche applicate allo studio della patogenicità microbica
SEMINARI:
verranno svolti dei seminari su alcune tematiche del modulo in esame da ricercatori interni e/o esterni al dipartimento impegnati in progetti di ricerca in ambito microbiologico.

The number of laboratory animal models in comparative research has expanded in quantity thanks to the application of biotechnologies to laboratory rodents that have permitted the creation of transgenic animals. Scientific personnel of various disciplines are involved in the production and study of laboratory animal models. These different professions need to integrate their specialties in team based collaboration.
Objective of the course is to provide the students of biotechnological sciences with insights to the adequate approach to the comprhenesion of most utilized animal models . The course will illustrate and explain major comparative spontaneous and experimental models of disease from the perspective of pathology

COURSE OBJECTIVES:
Teaching objectives of the course are to provide the students with the technical and cultural knowledge to the understanding of anatomic pathology of laboratory animals. The course provides information on major spontaneous disease of laboratory rodents, of genetically modified mice and the use of laboratory rodents in research from the perspective of the anatomical pathologist.

ORGANIZATION OF THE COURSE:
The course will be held by the responsible aided by pathologists working in different fields with laboratory animals (academia, private research centers, pharmaceutical industry, contract laboratories).

LECTURES:
· The role of the veterinary pathologist in laboratory animal studies
· Most utilized animal models.
· Transgenic mice: production and characterization.
· Trangenic mouse models of infecitous diseases.
· Principles of phenotyping of transgenic mice
· Nomenclature of mouse strains and genetically modified mice.
· Main spontaneous diseases (environmental, aging, infectious, neoplastic) in laboratory rodents
· Main diseases of rodent laboratory strains
· Immune deficient mice: infectious diseases

PRACTICAL TEACHING:
Most practical teaching will be performed at the microscope and seminars held by specialists of the filed.