Protein engineering and proteomics
A.A. 2018/2019
Obiettivi formativi
The course provides an introduction to protein biotechnology and the basic knowledge required for set up large-scale protein production within biotechnological industry, from cell cultivation in bioreactors up to protein purification in a preparative scale and includes different modern bioanalytical techniques. The course is aimed at giving students in-depth understanding of 1) the structure-function relationship of proteins and of 2) the methodologies used to produce, characterize and use proteins, enzymes included, and 3) the molecular basis and protein engineering tools available for designing enzymes or proteins with new or desirable functions. The course will have a strong hands-on connotation based on practical sessions either of experimental design in classroom and of bioinformatics.
Risultati apprendimento attesi
"At the end of the course the student will be able to
implement the notions of molecular biology, proteomics and
genetic engineering for the production and improvement of
proteins and enzymes. The student will be able to analyze
the structure of proteins and their post-translational
modifications; review the main factors that are significant
for protein folding processes and stability; explain how
proteins can be used for different industrial applications,
carry out mutagenesis approaches to improve protein
stability and to confer on them new functions."
implement the notions of molecular biology, proteomics and
genetic engineering for the production and improvement of
proteins and enzymes. The student will be able to analyze
the structure of proteins and their post-translational
modifications; review the main factors that are significant
for protein folding processes and stability; explain how
proteins can be used for different industrial applications,
carry out mutagenesis approaches to improve protein
stability and to confer on them new functions."
Periodo: Secondo semestre
Modalità di valutazione: Esame
Giudizio di valutazione: voto verbalizzato in trentesimi
Corso singolo
Questo insegnamento non può essere seguito come corso singolo. Puoi trovare gli insegnamenti disponibili consultando il catalogo corsi singoli.
Programma e organizzazione didattica
Edizione unica
Responsabile
Periodo
Secondo semestre
Programma
Structural/functional molecular characteristics of proteins: from biosynthesis to folding. Structural and functional dynamics of proteins. Modern approaches for the study of the proteins. Implementation of protein purification procedures. Proteome analyses for protein and enzyme identification and exploitation. Recombinant protein technologies; gene expression: vectors, expression in bacteria, yeasts, plants, insect and mammalian cells. Cell-Free translation systems. Inclusion bodies, their solubilization; protein refolding. Co-expression and use of chaperones. Storage stabilization of proteins. Hints of evolutionary biotechnology (rational design and directed evolution). Examples of tailored enzymes in industrial biocatalysis. Production and applications of polyclonal and monoclonal antibodies. Strategies to set up a protein engineering project.
Informazioni sul programma
Oral. At least 7 exam sessions per year will be guaranteed. The date of the exam will be published on SIFA platform.
Propedeuticità
None
Prerequisiti
General knowledge of structural biochemistry (protein primary, secondary, tertiary quaternary structures, including basics of protein folding) and cell protein synthesis. Enzymatic activities. Basics of gene cloning and heterologous gene expression. For any question and further details please contact the teacher at [email protected].
Materiale di riferimento
Reference material (books and relevant scientific papers) will be presented during the first lesson. Slides of the lessons will be available to students through ARIEL platform.
BIO/10 - BIOCHIMICA - CFU: 6
Esercitazioni: 16 ore
Laboratori: 8 ore
Lezioni: 36 ore
Laboratori: 8 ore
Lezioni: 36 ore
Docente:
Scarafoni Alessio
Docente/i
Ricevimento:
Giovedì, esclusivamente su appuntamento (e-mail)
edificio 21040, primo piano