Genetics, cellular and molecular biology

A.A. 2019/2020
12
Crediti massimi
96
Ore totali
SSD
BIO/11 BIO/13 BIO/18
Lingua
Inglese
Obiettivi formativi
The course introduces the fundamentals concepts in Cellular Biology, Molecular Biology, and Genetics.
Risultati apprendimento attesi
Students will acquire an operative understanding on genetics, cellular, and molecular biology fundamentals and in order to be able to apply the knowledge acquired to the interpretation of the results of the bioinformatic analysis of biomolecular data.
Programma e organizzazione didattica

Edizione unica

Responsabile
Periodo
Primo semestre
Programma
Cellular Biology:

1: Outer cellular structures: Biological membranes, cell walls and flagella
2: Cell biology of Anabolism: The Nucleus, the Endoplasmic Reticulum and the Golgi apparatus
3: Cell biology of Catabolism: The endo-lysosomal system and autophagy
4: Cellular energy and detox: Mitochondria, Chloroplasts and Peroxisomes
5: Intracellular logistics: The cytoskeletons, transport routes and motor systems
6: Elements of multicellular organization: From biofilms to cell specialization, cell-cell communication and stem cells
7: The cell in time: Cell cycles, cell death and senescence
8: Organelles and disease + Wrap up and synthesis

Genetics:

1: Introduction to Genetics
2: Mendelian Genetics I
3: Mendelian Genetics II
4: Principles of human genetics
5: Exercises
5: The structure of DNA, and Chromosomes.
6: The structure of chromatin
7: The structure of genes.
8: The genetic code.
9: Mutations and polymorphisms
10: Models of regulation (the Operon model)
11: Hybridization, amplification and NGS techniques.
12: Cancer Genetics
13: Population Genetics
14: Exercises
15: Principles of Epigenetics/Imprinting

Molecular Biology:

1- Genes and genome
2- DNA replication
3- Repair Mechanisms
4- Transcriptional regulation in procaryotes
5-Transcriptional regulation in eukaryotes
6- Splicing mechanisms
7- mRNA processing
8- Translation and translational regulation
9- Post- translational regulation
10- Molecular mechanisms of cell-cycle
11- ncRNA
12- Methods- plasmids and cloning
13- Methods- Northern, Southern, Western, PCR
14- Methods- DNA insertion in cell culture and different animal modes
15- Methods- CRISPR-CAS9, genome editing
Prerequisiti
No prerequisites different from those required for admission to the Master Degree program.
Metodi didattici
Lectures
Materiale di riferimento
Slides used during classes will be shared with students.
Textbooks covering the topics of the classes will be suggested by teachers.
Modalità di verifica dell’apprendimento e criteri di valutazione
Written and oral tests.
BIO/11 - BIOLOGIA MOLECOLARE - CFU: 0
BIO/13 - BIOLOGIA APPLICATA - CFU: 0
BIO/18 - GENETICA - CFU: 0
Lectures: 96 ore
Docente/i
Ricevimento:
Venerdì ore 16,30
Dipartimento Bioscienze Via Celoria 26
Ricevimento:
Thursday - Giovedì 17-18 (previo appuntamento)
office 2b