The course introduces the fundamentals concepts in Cellular Biology, Molecular Biology, and Genetics.
Expected learning outcomes
Students will acquire an operative understanding on genetics, cellular, and molecular biology fundamentals and in order to be able to apply the knowledge acquired to the interpretation of the results of the bioinformatic analysis of biomolecular data.
Lesson period: First semester
(In case of multiple editions, please check the period, as it may vary)
GOALS The course introduces the fundamentals concepts in Cellular Biology, Molecular Biology, and Genetics.
1: Outer cellular structures: Biological membranes, cell walls and flagella 2: Cell biology of Anabolism: The Nucleus, the Endoplasmic Reticulum and the Golgi apparatus 3: Cell biology of Catabolism: The endo-lysosomal system and autophagy 4: Cellular energy and detox: Mitochondria, Chloroplasts and Peroxisomes 5: Intracellular logistics: The cytoskeletons, transport routes and motor systems 6: Elements of multicellular organization: From biofilms to cell specialization, cell-cell communication and stem cells 7: The cell in time: Cell cycles, cell death and senescence 8: Organelles and disease + Wrap up and synthesis
1: Introduction to Genetics 2: Mendelian Genetics I 3: Mendelian Genetics II 4: Principles of human genetics 5: Exercises 5: The structure of DNA, and Chromosomes. 6: The structure of chromatin 7: The structure of genes. 8: The genetic code. 9: Mutations and polymorphisms 10: Models of regulation (the Operon model) 11: Hybridization, amplification and NGS techniques. 12: Cancer Genetics 13: Population Genetics 14: Exercises 15: Principles of Epigenetics/Imprinting
1- Genes and genome 2- DNA replication 3- Repair Mechanisms 4- Transcriptional regulation in procaryotes 5-Transcriptional regulation in eukaryotes 6- Splicing mechanisms 7- mRNA processing 8- Translation and translational regulation 9- Post- translational regulation 10- Molecular mechanisms of cell-cycle 11- ncRNA 12- Methods- plasmids and cloning 13- Methods- Northern, Southern, Western, PCR 14- Methods- DNA insertion in cell culture and different animal modes 15- Methods- CRISPR-CAS9, genome editing
REFERENCE MATERIAL Slides used during classes will be shared with students. Textbooks covering the topics of the classes will be suggested by teachers.
PREREQUISITES AND EXAMINATION PROCEDURES Written and oral tests.