Structural biology and enzymology

A.A. 2019/2020
6
Crediti massimi
48
Ore totali
SSD
BIO/10
Lingua
Inglese
Obiettivi formativi
Verranno illustrati i principi del Protein Engineering, una tecnica emergente che consente la sintesi e la produzione di sistemi proteici modificati, utilizzati sia per elucidare meccanismi di reazioni biologiche sia per applicazioni in campo biotecnologico.
Risultati apprendimento attesi
Ci si aspetta che gli studenti abbiano sviluppato i concetti di base delle relazioni struttura-funzione proteica e catalisi enzimatica, comprendendo la logica degli attuali approcci e metodi di ingegneria proteica ed acquisendo la capacita' di correlare le connessioni teoriche e sperimentali tra le discipline coinvolte e comprendere la loro rilevanza per gli sviluppi biotecnologici. Devono anche aver acquisito il vocabolario tecnico necessario per leggere criticamente gli articoli di ricerca e presentarli in forma orale e scritta.
Programma e organizzazione didattica

Edizione unica

Programma
The lectures will first review information on: (i) protein structure, folding and structure-function relations, (ii) the principles of enzyme catalysis and kinetic tools to study enzyme catalytic properties; (iii) spectroscopic techniques for the study of proteins and their function; (iv) techniques and strategies for the overexpression, purification and modification of molecular targets, especially proteins and enzymes.
Lectures. will then be largely based on the discussion of articles from specialized journals, and will cover:
- protein engineering goals and strategies
- design, expression, selection, isolation, and characterization of protein variants.
Examples of successful protein engineering experiments will be discussed during classes and made available to students.
Prof. Nardini and Prof. Vanoni will share the teaching of this class by focusing on structural biology and enzymology aspects applied to protein engineering, respectively.
Prerequisiti
A revision of the topics covered by the basic biochemistry and molecular biology classes included in the bachelor curriculum before attending the course is highly recommended.
Metodi didattici
Teaching Mode: Classroom lectures supported by projected material with common discussions on experimental design, data analysis, and specific case studies. Attendance is highly recommended.
Materiale di riferimento
Voet, D. & Voet, J.G Biochemistry , 4th Edition, J. Wiley & Sons
· Fersht, A. Structure and mechanism in protein science (Freeman)
· Frey, P.A. & Hegeman, A.D. Enzymatic Reaction Mechanisms, Oxford University Press (2007)
· Petsko, G.A. & Ringe, D. Protein structure and function, New Science Press, 2004
Articles on which lectures will be based.

Copies of the slides projected in the classroom as well as other materials will be made available through the course website on the ARIEL platform of the University of Milano (https://mavanonipeme.ariel.ctu.unimi.it/v5/Home). By no means this material replaces the lectures or a textbook. The material is made available only to registered students of the Degree Course in Molecular Biotechnology and Bioinformatics and should not be distributed to others.
Modalità di verifica dell’apprendimento e criteri di valutazione
The evaluation of the student's performance will be based on a "journal club" activity and a written examination with open-answer questions spanning all topics covered in the class.
For the "journal club" activity students will work in small groups. Presentations will be done at the end of the class and before the written exam. The evaluation of the Journal club activity will account for up to 2 points of the final grade. Students will have the possibility (if they wish) to integrate the written exam with an oral discussion of the written paper to clarify their actual mastering of the topics.
The final grade will result from the joint evaluation of each candidate by the two teachers.
BIO/10 - BIOCHIMICA - CFU: 6
Lezioni: 48 ore
Docente/i
Ricevimento:
Giovedi, ore 10:30-12:30
Dip. di Bioscienze, Torre C, 5 piano
Ricevimento:
Lunedi' ore 13-14
Unita' di Biochimica delle proteine, Edifici Biologici, Via Celoria 26, 5C