Bioimaging
A.A. 2023/2024
Obiettivi formativi
The imaging of biological samples, or bioimaging, plays a key role in current life science research, enabling scientists to analyze molecules, cells and tissues from a range of living systems. Developments in microscopy techniques and associated tools now allow imaging across an extensive range of scales, from 1-2 nm to whole organism phenotyping.
The aim of the course is to present the theory and the practice of different techniques of microscopy (optical, electronic and scanning probe) also applied in lab-on-a-chip systems.
The course is ideally linked to those dealing with the need of imaging, i.e. molecular and cellular biology, etc.
The aim of the course is to present the theory and the practice of different techniques of microscopy (optical, electronic and scanning probe) also applied in lab-on-a-chip systems.
The course is ideally linked to those dealing with the need of imaging, i.e. molecular and cellular biology, etc.
Risultati apprendimento attesi
After following this course, the students will acquire basic know-how in microscopy and the capability to select suitable approaches to image biological samples.
Periodo: Primo semestre
Modalità di valutazione: Esame
Giudizio di valutazione: voto verbalizzato in trentesimi
Corso singolo
Questo insegnamento può essere seguito come corso singolo.
Programma e organizzazione didattica
Edizione unica
Responsabile
Periodo
Primo semestre
Programma
Part 1 (C. Lenardi)
The first part of the course provides a description of the imaging techniques applied to biology. Some basic notions of geometrical optics are functional to the detailed description of optical microscopes. Confocal microscopy and related techniques (FRAP, FRET, FLIM) will be extensively treated together with more recent developments in super-resolution techniques, in particular SIM, PALM, STORM, STED. Electron microscopies (SEM, TEM, Cryo-TEM) will be presented underlying the different investigations on biological samples that can be carried out. Atomic Force Microscopy (AFM) will be also presented as a versatile approach for measuring mechanical properties of biological samples.
Part 2 (M. Piazzoni)
The second part of the course deals with the topic of in vitro quantitative cell and tissue biology.
An overview of physic laws governing small systems will be highlighted and explained through Lab-on-chip (LOC) devices, that are laboratory functionalities integrated into miniaturized devices. Next, biomaterials commonly employed as scaffolds in tissue engineering and the related fabrication technologies (focusing on 3D printing) will be presented together with those methodologies used to modify their chemical surface properties. In the end, some examples of engineered 3D cell cultures, such as the muscle and the bone tissues, will be deeply discussed and practical problems will be presented.
The first part of the course provides a description of the imaging techniques applied to biology. Some basic notions of geometrical optics are functional to the detailed description of optical microscopes. Confocal microscopy and related techniques (FRAP, FRET, FLIM) will be extensively treated together with more recent developments in super-resolution techniques, in particular SIM, PALM, STORM, STED. Electron microscopies (SEM, TEM, Cryo-TEM) will be presented underlying the different investigations on biological samples that can be carried out. Atomic Force Microscopy (AFM) will be also presented as a versatile approach for measuring mechanical properties of biological samples.
Part 2 (M. Piazzoni)
The second part of the course deals with the topic of in vitro quantitative cell and tissue biology.
An overview of physic laws governing small systems will be highlighted and explained through Lab-on-chip (LOC) devices, that are laboratory functionalities integrated into miniaturized devices. Next, biomaterials commonly employed as scaffolds in tissue engineering and the related fabrication technologies (focusing on 3D printing) will be presented together with those methodologies used to modify their chemical surface properties. In the end, some examples of engineered 3D cell cultures, such as the muscle and the bone tissues, will be deeply discussed and practical problems will be presented.
Prerequisiti
None
Metodi didattici
The lessons will be delivered by means of slides. Visits to the Imaging platform (NOLIMITS-UNITECH), to the scanning probe microscopy (SPM) laboratory (Dept. of Physics) and to Advanved Biomaterials platform (Fondazione UNIMI) are scheduled. The slides are uploaded to ARIEL.
Materiale di riferimento
1] G. Haugstad, Atomic force microscopy: understanding basic modes and advanced applications, John Wiley & Sons, Hoboken, N.J, 2012.
[2] U. Kubitscheck, ed., Fluorescence microscopy: from principles to biological applications, Wiley-Blackwell, Weinheim, 2013.
[3] R.F. Egerton, Physical principles of electron microscopy: an introduction to TEM, SEM, and AEM, second edition, Springer, Cham, 2016.
[4] Tissue Engineering, Edited By Bernhard Palsson, Jeffrey A. Hubbell, Robert Plonsey, Joseph D. Bronzino, CRC Press 2019.
Copies of the slides projected in the classroom as well as other materials will be made available through the course website on the ARIEL platform of the University of Milano (https://clenardib.ariel.ctu.unimi.it/v5/home/Default.aspx). By no means this material replaces the lectures or a textbook. The material is made available only to registered students of the Degree Course in Molecular Biotechnology and Bioinformatics and should not be distributed to others.
[2] U. Kubitscheck, ed., Fluorescence microscopy: from principles to biological applications, Wiley-Blackwell, Weinheim, 2013.
[3] R.F. Egerton, Physical principles of electron microscopy: an introduction to TEM, SEM, and AEM, second edition, Springer, Cham, 2016.
[4] Tissue Engineering, Edited By Bernhard Palsson, Jeffrey A. Hubbell, Robert Plonsey, Joseph D. Bronzino, CRC Press 2019.
Copies of the slides projected in the classroom as well as other materials will be made available through the course website on the ARIEL platform of the University of Milano (https://clenardib.ariel.ctu.unimi.it/v5/home/Default.aspx). By no means this material replaces the lectures or a textbook. The material is made available only to registered students of the Degree Course in Molecular Biotechnology and Bioinformatics and should not be distributed to others.
Modalità di verifica dell’apprendimento e criteri di valutazione
The exam consists of: 1) presentation with slides of a paper assigned by the teachers concerning recent studies in the biotechnology field in which at least one of the imaging techniques described during the course are used (max 20 min);
2) oral exam concerning the topics covered in the course, in particular a part will deal with the principles and istruments for bioimaging and a part concerning the application of imaging techniques in lab-on-chips.
Examples of the examination test will be discussed during classes and made available to students.
All the theoretical and practical lectures will be delivered if possible in the class-room and, if required, synchronously on ZOOM platform. Archive and Notices on Ariel.
2) oral exam concerning the topics covered in the course, in particular a part will deal with the principles and istruments for bioimaging and a part concerning the application of imaging techniques in lab-on-chips.
Examples of the examination test will be discussed during classes and made available to students.
All the theoretical and practical lectures will be delivered if possible in the class-room and, if required, synchronously on ZOOM platform. Archive and Notices on Ariel.
FIS/03 - FISICA DELLA MATERIA
FIS/07 - FISICA APPLICATA (A BENI CULTURALI, AMBIENTALI, BIOLOGIA E MEDICINA)
FIS/07 - FISICA APPLICATA (A BENI CULTURALI, AMBIENTALI, BIOLOGIA E MEDICINA)
Esercitazioni: 16 ore
Lectures: 40 ore
Lectures: 40 ore
Docenti:
Lenardi Cristina, Piazzoni Marco
Siti didattici
Docente/i
Ricevimento:
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