Methods in ecotoxicology
A.A. 2023/2024
Obiettivi formativi
The main objectives of the course are:
1. to provide notions for a correct characterization and interpretation of the environmental distribution and the toxic effects of xenobiotics on the ecosystems;
2. to provide notions on the approaches that can be applied to characterize the molecular mechanisms of action of xenobiotics;
3. to provide notions on hazard identification and risk assessment of environmental pollutants.
1. to provide notions for a correct characterization and interpretation of the environmental distribution and the toxic effects of xenobiotics on the ecosystems;
2. to provide notions on the approaches that can be applied to characterize the molecular mechanisms of action of xenobiotics;
3. to provide notions on hazard identification and risk assessment of environmental pollutants.
Risultati apprendimento attesi
It is expected that at the end of the course the student will be able to understand the different possible interactions between environmental contaminants and biota, including humans, and methods currently available to determine such interactions. The students should acquire notions for a correct characterization and interpretation of the environmental distribution and the toxic effects of xenobiotics on the ecosystems.
Periodo: Primo semestre
Modalità di valutazione: Esame
Giudizio di valutazione: voto verbalizzato in trentesimi
Corso singolo
Questo insegnamento può essere seguito come corso singolo.
Programma e organizzazione didattica
Edizione unica
Responsabile
Periodo
Primo semestre
Programma
The teaching, divided in two parts, aims at explain the main methodological approaches used in ecotoxicology to study the effects of environmental contaminants.
After a description of 3Rs (Reduce, Refine, Replace) and in vitro approach in ecotoxicology, the first part of the teaching will describe the main in vitro tests end relative endpoints, their application also through integrated strategies and evaluation of emerging contaminants. Practical activity in lab will focus on in vitro toxicological approach and Non Animal Methods (NAMs).
The second part of the teaching will focus on the description of In vivo approaches, models and techniques used in standardized ecotoxicological tests, as well as on the application of biochemical and behavioral biomarkers, to explore the effects and the mechanism(s) of action of contaminants. Case studies of in vivo approach for ecotoxicity assessment of emerging contaminants will be provided. Practical activity in the lab will be aimed at the application of in vivo approaches and techniques on a freshwater model organism to assess the toxicity of an emerging contaminant.
After a description of 3Rs (Reduce, Refine, Replace) and in vitro approach in ecotoxicology, the first part of the teaching will describe the main in vitro tests end relative endpoints, their application also through integrated strategies and evaluation of emerging contaminants. Practical activity in lab will focus on in vitro toxicological approach and Non Animal Methods (NAMs).
The second part of the teaching will focus on the description of In vivo approaches, models and techniques used in standardized ecotoxicological tests, as well as on the application of biochemical and behavioral biomarkers, to explore the effects and the mechanism(s) of action of contaminants. Case studies of in vivo approach for ecotoxicity assessment of emerging contaminants will be provided. Practical activity in the lab will be aimed at the application of in vivo approaches and techniques on a freshwater model organism to assess the toxicity of an emerging contaminant.
Prerequisiti
Principles of ecology, toxicology, ecotoxicology are required.
Metodi didattici
Course delivery methods will be based on interactive lectures and practical activity in laboratory. The student will actively partecipate in the discussion of the topics to improve the critical skills.
Materiale di riferimento
Didactic material available on ARIEL
Textbook:
François Gagné, 2014. Biochemical Ecotoxicology. Principles and Methods. Elsevier eds.
Textbook:
François Gagné, 2014. Biochemical Ecotoxicology. Principles and Methods. Elsevier eds.
Modalità di verifica dell’apprendimento e criteri di valutazione
Student learning assessment will take place through an oral examination and knowledge of course topics will be evaluated.
BIO/07 - ECOLOGIA
VET/07 - FARMACOLOGIA E TOSSICOLOGIA VETERINARIA
VET/07 - FARMACOLOGIA E TOSSICOLOGIA VETERINARIA
Esercitazioni: 32 ore
Lezioni: 32 ore
Lezioni: 32 ore
Docenti:
Caloni Francesca, Parolini Marco
Siti didattici
Docente/i
Ricevimento:
Previo appuntamento via mail
Via Celoria 10 - Milano
Ricevimento:
da concordare previo appuntamento richiesto via e-mail