Analytic Chemistry Ii with Lab

Goals
Instrumentation and principles of the main analytical and separation techniques. Resolution of common qualitative and quantitative problems.

acquired skills
At the end of the course the student is able to solve small analytical problems such as quantitative with the choice of the most suitable instrumental technique. Ability to identify the structure of simple organic compounds from their IR, NMR and MS spectra.

Principles of spectrophotometric and photoelectronic methods. Atomic adsorption and emission. Molecular spectroscopy: UV-vis, IR and RAMAN, fluorescence and phosphorescence.
Mass spectrometry.
Nuclear Magnetic Resonance: monodimensional 1H- and 13C-NMR techniques. Spectrum parameter analyses: chemical shift and coupling constant. Overhauser effect.
Principles and methods of chromatographic techniques (GC and HPLC).
Set-up of qualitative and quantitative analyses.
Exercises on structure disclosing based on NMR, IR, UV and MS spectra. Practices of theoretical concepts. Numerical exercises on quantitative analyses involving spectroscopic and chromatographic techniques.
Laboratory
Qualitative and quantitative experiences on the main spectroscopic (AAS, AES, UV-Vis, IR, fluorescence) and chromatographic (GC, HPLC) techniques presented during the lectures.

Reference materials
- Holler, Skoog, Crouch "Chimica Analitica Strumentale" EdiSES,
- H. Friebolin, Basic One- and Two-Dimensional NMR Spectroscopy, VCH;
- R.M. Silverstein, F.X. Webster, Identificazione spettroscopica di composti organici, Ed. CEA
- Dispense dei docenti

Assessment method:
The exam includes two tests: a theory that contains questions on the theory and interpretation of spectra, the other on the experiences in the laboratory