General Microbiology

A.y. 2018/2019
9
Max ECTS
80
Overall hours
SSD
BIO/19
Language
Italian
Learning objectives
Il corso si propone di fornire allo studente le conoscenze di base in campo microbiologico, le competenze necessarie all'utilizzo di metodologie microbiologiche connesse alla biotecnologia. Vengono forniti elementi e approfondimenti di struttura cellulare, fisiologia e genetica microbica, di potenzialità applicative dei microrganismi nella biotecnologia e di studio degli organismi patogeni e dei loro meccanismi di virulenza.
Expected learning outcomes
Undefined
Course syllabus and organization

Linea 1

Responsible
Lesson period
First semester
Course syllabus
Characteristics of prokaryotic and eukaryotic microorganisms, morphology of bacteria and eumycetes
Nutritional requisites of prokaryotes and culture methods; identification with 16S rRNA and molecular taxonomy
Bacterial growth and direct / indirect methods for assessing growth
Growth control: antibiotics

Cytology: membrane, cell wall, cytoplasm
Structural and biochemical differences between Bacteria / Archea
Structural and biochemical differences between Gram positive and Gram negative bacteria
Sporulation
The fundamental biological processes in Bacteria: DNA replication, transcription, translation cell wall biosynthesis
Mechanisms of gene regulation in response to nutritive availability: cAMP, ppGpp
Two-component regulation systems: regulation of flagellar motility
Microbial genetics: gene transfer: conjugation transformation, mobile genetic elements, transduction
Elements of virology: bacteriophages, examples of animal viruses (retrovirus, RNA viruses), mechanisms of infection and interaction with host cells
Antibiotics: mechanisms of action and resistance

Microbial metabolism: aerobic respiration, anaerobic respiration, fermentation
Oxygenic and anoxic photosynthesis, assimilation of CO2
Nitrogen Fixing
Mycete classification, life cycle, nutrition
Biogeochemical cycles (C, N, P, S)

Laboratory activities (1CFU = 16 HOURS)
Plating and isolation of pure cultures of microorganisms. Selective growth conditions (anaerobiosis, minimal and differential terrain). Growth curves and antibiotic sensitivity. Microscopy: observation of live cells, Gram staining. Antibiotic susceptibility tests.
BIO/19 - MICROBIOLOGY - University credits: 9
Single bench laboratory practical: 16 hours
Lessons: 64 hours
Shifts:
Professor: Landini Paolo
Turno 1
Professor: Paroni Moira
Turno 2
Professor: Paroni Moira

Linea 2

Responsible
Lesson period
First semester
BIO/19 - MICROBIOLOGY - University credits: 9
Single bench laboratory practical: 16 hours
Lessons: 64 hours
Professor: Borin Sara
Shifts:
Professor: Borin Sara
Turno 1
Professor: Borin Sara
Turno 2
Professor: Borin Sara
Professor(s)
Reception:
everyday under appointment
office: via Mangiagalli 25, 3rd floor, Environmental Microbiology Section
Reception:
Thursdays 15.30-16.30
Via Celoria 26, Scientific buildings, 4th floor, Tower "B"