Methods in Cytochemistry
A.Y. 2018/2019
Learning objectives
The aim of the course is to provide students with the basic principles of cytochemistry and histochemistry. The preparation techniques for sample analysis used in cytochemistry, histochemistry, immunocytochemistry and in situ hybridization will be illustrated. Basic knowledge will also be provided for optical and electronic visualization of biological interest macromolecules. Theoretical lessons will be associated with seminars / workshops relating to examples of application of these methods to the study of cellular biology and instrument observation tools.
Expected learning outcomes
Undefined
Lesson period: Second semester
Assessment methods: Esame
Assessment result: voto verbalizzato in trentesimi
Single course
This course cannot be attended as a single course. Please check our list of single courses to find the ones available for enrolment.
Course syllabus and organization
Single session
Responsible
Course syllabus
- Definition of the discipline and presentation of fields of study of cytochemistry and histochemistry in human field.
- The processing of the samples: from the fixation to the microscopic observation (collecting samples, fixation, inclusion, cutting, stains, dyes).
- Instruments for microscopic observations.
- Histochemical base reactions for Carbohydrates, Proteins, Lipids and Nucleic acids.
- Immunohistochemical reactions
- The fluorochromes and fluorescence microscopy.
- The techniques of in situ hybridization. Fluorescent in situ hybridization (FISH).
- Applications in basic and applied research.
Reference Material
A.G.E. Pearse, Istochimica. Teoria e pratica, Piccin.
E. Bonucci, Manuale di Istochimica, Lombardo.
K.M. Polak and S. Van Noorden, Introduction to Immunocytochemistry
Prerequisites and examination procedures
The examination will take place in written form, with open questions or in oral form.
Teaching Methods
Traditional
Language of instruction
Italian
Recommended Prerequisites
Students must be fully proficient (i.e. they are asked to have passed the respective first year exams) in Cytology and histology and in Biological chemistry.
Program information
Program information and pdf copies of slides projected in class will be available on the teacher website of online education. By no means these materials substitute for the lectures or a textbook. The material is made available only to registered students of the Degree Course in Biological Sciences and should not be distributed to others.
WEB pages
http://mpasinimc.ariel.ctu.unimi.it/v3/home/Default.aspx
- The processing of the samples: from the fixation to the microscopic observation (collecting samples, fixation, inclusion, cutting, stains, dyes).
- Instruments for microscopic observations.
- Histochemical base reactions for Carbohydrates, Proteins, Lipids and Nucleic acids.
- Immunohistochemical reactions
- The fluorochromes and fluorescence microscopy.
- The techniques of in situ hybridization. Fluorescent in situ hybridization (FISH).
- Applications in basic and applied research.
Reference Material
A.G.E. Pearse, Istochimica. Teoria e pratica, Piccin.
E. Bonucci, Manuale di Istochimica, Lombardo.
K.M. Polak and S. Van Noorden, Introduction to Immunocytochemistry
Prerequisites and examination procedures
The examination will take place in written form, with open questions or in oral form.
Teaching Methods
Traditional
Language of instruction
Italian
Recommended Prerequisites
Students must be fully proficient (i.e. they are asked to have passed the respective first year exams) in Cytology and histology and in Biological chemistry.
Program information
Program information and pdf copies of slides projected in class will be available on the teacher website of online education. By no means these materials substitute for the lectures or a textbook. The material is made available only to registered students of the Degree Course in Biological Sciences and should not be distributed to others.
WEB pages
http://mpasinimc.ariel.ctu.unimi.it/v3/home/Default.aspx
BIO/16 - HUMAN ANATOMY - University credits: 6
Lessons: 36 hours
Lessons (seminars): 12 hours
Lessons (seminars): 12 hours
Professor:
Ricciardi Sara
Professor(s)