Omics

A.Y. 2018/2019
10
Max ECTS
66
Overall hours
SSD
BIO/10 BIO/18
Language
Italian
Learning objectives
The "Introduction to genomics" module presents to the students the main concepts dealing with genome organization. In facts a graded student in Veterinary Biotechnologies must have basic knowledges on the dimension and architecture of genome and chromosomes, in addition to the meaning of linkage and linkage disequilibrium between loci. Practical applications for animal breeding and veterinary medicine are also presented with the connected doctrinal and statistical tools, for example the use of bead DNA chip for genomic evaluation in cattle, parental exclusion or for general concepts and application in population genomics (F-statistics).
The Proteomic module presents essential information to study the complete proteome of a cell or tissue, its qualitatively and quantitative characterization and the analysis of possible modifications induced by a stimulus. Many different applications of this approach will be illustrated starting from published data.
Expected learning outcomes
Undefined
Course syllabus and organization

Single session

Responsible
Lesson period
First semester
ATTENDING STUDENTS
Unita' didattica: Proteomica
Course syllabus
MODULE OBJECTIVES:
The course is aimed at providing information concerning the principal biochemical techniques utilized in proteomics

Lectures
Protein identification and analysis
Cellular and sub-cellular extracts
Analytical and preparative electrophoresis with pH immobilized gradients
Staining methods
Protein maps and sequence analysis of proteins separated in gel
Sequence of proteins and peptides in solution
Amino acids analysis of proteins and peptides
Microarrays of proteins
Mass spectrometry in proteomics
Quantitative analysis by mass spectrometry
Studies of post-translational modifications by mass spectrometry
Protein complexes characterization
Comparative proteomics
Biomarkers and antigens identification, food quality control
Microorganisms identifications by mass spectrometry
Spectroscopic techniques in proteomics (IR, NMR, EPR)
NON-ATTENDING STUDENTS
Unita' didattica: Proteomica
Course syllabus
MODULE OBJECTIVES:
The course is aimed at providing information concerning the principal biochemical techniques utilized in proteomics

Lectures
Protein identification and analysis
Cellular and sub-cellular extracts
Analytical and preparative electrophoresis with pH immobilized gradients
Staining methods
Protein maps and sequence analysis of proteins separated in gel
Sequence of proteins and peptides in solution
Amino acids analysis of proteins and peptides
Microarrays of proteins
Mass spectrometry in proteomics
Quantitative analysis by mass spectrometry
Studies of post-translational modifications by mass spectrometry
Protein complexes characterization
Comparative proteomics
Biomarkers and antigens identification, food quality control
Microorganisms identifications by mass spectrometry
Spectroscopic techniques in proteomics (IR, NMR, EPR)
Unita' didattica: Introduzione alla genomica
BIO/10 - BIOCHEMISTRY - University credits: 0
BIO/18 - GENETICS - University credits: 0
Lessons: 18 hours
Professor: Pagnacco Giulio Giorgio Alessandro
Unita' didattica: Proteomica
BIO/10 - BIOCHEMISTRY - University credits: 0
BIO/18 - GENETICS - University credits: 0
Practicals: 12 hours
Lessons: 36 hours
Professor: Tedeschi Gabriella