Research strategies and methodologies applied to disease study and control

A.Y. 2018/2019
8
Max ECTS
66
Overall hours
SSD
VET/05 VET/06
Language
Italian
Learning objectives
The main objectives of the course are: to provide students with information on the main traditional and advanced biomolecular techniques to be used in the parasitological field; to provide students with concepts related to the transformation, use and management of transgenic mice.
Expected learning outcomes
Undefined
Course syllabus and organization

Single session

Responsible
Lesson period
First semester
ATTENDING STUDENTS
Unita' didattica: Biotecnologie avanzate in parassitologia
Course syllabus
Organization of the course:
1) reading scientific papers and writing material & methods
2) designing research projects
3) how to handle and store biological samples
4) research strategies; molecular biology and parasitology landmarks
5) principal molecular biology techniques
6) Next generation sequencing applications
7) recombinant proteins for immunological studied and vaccines productions
Unita' didattica: Modelli murini e cellulari geneticamente modificati
Course syllabus
Didattica frontale:
Gene targeting, Conditional gene targeting di proteine del sistema immunitario (2 ore)
Metodi di generazione di linee di topi transgenici (2 ore)
Cenni di legislazione in ambito di sperimentazione animale (2 ora)
In vivo imaging (2 ore)
Gestione di colonie murine geneticamente modificate (2 ore)
Tecnologie di modificazione genica di cellule in vitro (2 ore)

Esercitazioni
Genotipizzazione animali (5 ore)
Visita struttura di stabulazione (4 ore)
Disegno di un costrutto per gene targeting (3)

Lectures
Gene targeting, Conditional gene targeting of immune system proteins (2 hours)
Techniques for the production of transgenic mouse (2 hours)
Italian legislation on Animal welfare in research (2 hour)
In vivo imaging (2 hours)
Management of GM murine colonies (2 hours)
GM cells in vitro (2 Hours)

Exercises
Mouse genotyping (5 hours)
Gene targeting vector design (3 hours)
Visit of specialized laboratories and animal facilities (4 hours)
NON-ATTENDING STUDENTS
Unita' didattica: Biotecnologie avanzate in parassitologia
Course syllabus
Organization of the course:
1) reading scientific papers and writing material & methods
2) designing research projects
3) how to handle and store biological samples
4) research strategies; molecular biology and parasitology landmarks
5) principal molecular biology techniques
6) Next generation sequencing applications
7) recombinant proteins for immunological studied and vaccines productions
Unita' didattica: Modelli murini e cellulari geneticamente modificati
Course syllabus
Didattica frontale:
Gene targeting, Conditional gene targeting di proteine del sistema immunitario (2 ore)
Metodi di generazione di linee di topi transgenici (2 ore)
Cenni di legislazione in ambito di sperimentazione animale (2 ora)
In vivo imaging (2 ore)
Gestione di colonie murine geneticamente modificate (2 ore)
Tecnologie di modificazione genica di cellule in vitro (2 ore)

Esercitazioni
Genotipizzazione animali (5 ore)
Visita struttura di stabulazione (4 ore)
Disegno di un costrutto per gene targeting (3)

Lectures
Gene targeting, Conditional gene targeting of immune system proteins (2 hours)
Techniques for the production of transgenic mouse (2 hours)
Italian legislation on Animal welfare in research (2 hour)
In vivo imaging (2 hours)
Management of GM murine colonies (2 hours)
GM cells in vitro (2 Hours)

Exercises
Mouse genotyping (5 hours)
Gene targeting vector design (3 hours)
Visit of specialized laboratories and animal facilities (4 hours)
Unita' didattica: Biotecnologie avanzate in parassitologia
VET/06 - PARASITOLOGY AND ANIMAL PARASITIC DISEASES - University credits: 5
Practicals: 24 hours
Lessons: 18 hours
Professor: Bazzocchi Chiara
Unita' didattica: Modelli murini e cellulari geneticamente modificati
VET/05 - INFECTIOUS DISEASES OF DOMESTIC ANIMALS - University credits: 3
Practicals: 12 hours
Lessons: 12 hours
Professor: Riva Federica
Professor(s)
Reception:
Wednesday; 11.00-12.00
DIVET; General Pathology and Parasitology