In Vitro Model Technologies

lectures
Introduction to in vitro Toxicology in vitro (1 hour)
In vitro models and evaluation of bioavailability, transport, metabolism and toxicity (4 hour)
Nanotoxicology: in vitro toxicity of nanoparticles (1 hour)
Practical
Cytotoxicity assays: release of LDH, MTT test, Neutral Red Up-take (6 hours)
Evaluation of the release of interleukin 1 alpha (2 hours)
Evaluation of the release of TNF-alpha (2 hours)
Toxicity studies in vitro with 3D models (4 hours)
Epithelial barriers and in vitro permeability studies (4 hours)
Evaluation of TEER (Trans-Epithelial Electric Resistance) (2 hours)
Endocrine Disruptor and in vitro models (2 hours)
In vitro hepatotoxicity and metabolism (2 hours)

The course will provide a part of the educational scientific basis for setting up systems of selection, storage, conveyance and release of cell populations, autologous and heterologous mammalian appropriate animal organs or body structures, other functional elements for create neoorgani and pseudorgani aimed at scientific research and biotechnology "in vitro", aimed at studying the regulation and modulation of various biological activities. Through these models of organs, it will be possible to arrive at an objective assessment of any potential use "in vivo".
The program will be as follows:
Preparation and Treatment of samples
Determination of the biological activity of sex hormones
Characterization of secreted through laboratory testing