Animal Cell Biotechnology
A.Y. 2019/2020
Learning objectives
The course aims to provide students with a more exhaustive picture of the different ways of studying and using the cell for more applicative purposes: from the multiple methods to isolate and maintain different cell types in vitro and the different methods to generate and use it for biotechnological purposes, from the production of antibodies, viral vectors to the generation of genetically modified organisms. Part of the course will focus on animal transgenesis: what a model organism is, the methods for generating Knock-out and Knock-in animals, the Bioreactors. The course therefore provides a complete view of the current state of knowledge in the field of cell biotechnology.
Expected learning outcomes
At the end of the course the student will acquire the basic knowledge of how a cell can be isolated, used and modified for applicative purposes in the field of biotechnology and animal transgenesis.
The student will acquire the ability to describe the current approaches to isolate, modify and use the cell for more applicative purposes, using the correct terminology.
The student will acquire the ability to describe the current approaches to isolate, modify and use the cell for more applicative purposes, using the correct terminology.
Lesson period: Second semester
Assessment methods: Esame
Assessment result: voto verbalizzato in trentesimi
Single course
This course cannot be attended as a single course. Please check our list of single courses to find the ones available for enrolment.
Course syllabus and organization
Single session
Responsible
Lesson period
Second semester
Course syllabus
Cell Culrture: Definition, mantainance and characteristics of different types of cell cultures. Explants, primary and immortalized cell cultures. Suspension and adherent cell cultures. Characterization of cultured cells, expression of specific markers of differentiated cells. Cell Cultures for toxicity tests, cell transplantation. Cell cloning.
Introduction of exogenous DNA into mammalian cells: cloning, characteristics of the different vectors used for eukaryotic cells. Reporter systems. Different methods of transfections. Stable and transient transfection. Cell Transduction.
Subcellular localization of macromolecules: cell fractioning and immunofluorescence. Use and production of mono-and polyclonal antibodies: somatic hybridization.
GFP and its application in cell culture: subcellular localization of proteins, analysis of protein-protein interactions (FRET), analysis of the dynamics of protein (FRAP), photoactivable GFP.
Stem cells (embryonic and adult) and induced pluripotent stem cells (iPS): features and applications.
Animal models: mouse and zebrafish.
Transgenic mice: mouse knock-in, knock-out mice, conditional knock-out mice.
Bioreactors
Introduction of exogenous DNA into mammalian cells: cloning, characteristics of the different vectors used for eukaryotic cells. Reporter systems. Different methods of transfections. Stable and transient transfection. Cell Transduction.
Subcellular localization of macromolecules: cell fractioning and immunofluorescence. Use and production of mono-and polyclonal antibodies: somatic hybridization.
GFP and its application in cell culture: subcellular localization of proteins, analysis of protein-protein interactions (FRET), analysis of the dynamics of protein (FRAP), photoactivable GFP.
Stem cells (embryonic and adult) and induced pluripotent stem cells (iPS): features and applications.
Animal models: mouse and zebrafish.
Transgenic mice: mouse knock-in, knock-out mice, conditional knock-out mice.
Bioreactors
Prerequisites for admission
It is recommended to have acquired basic skills in cellular and molecular biology
Teaching methods
Teaching method based on interactive frontal lessons supported by projected material (slides as power point presentations). Students will be stimulated to actively participate in the lesson / discussion to improve their critical skills, analyzing the literature and communicating the concepts in appropriate way. Frequency mode: strongly recommended
Teaching Resources
Slides on Ariel Professor web-site
Course books:to be defined and indicated yearly at the beginning of the teaching.
Course books:to be defined and indicated yearly at the beginning of the teaching.
Assessment methods and Criteria
The exam will be divided into a first written part with open and multiple choice questions and a following oral evaluation. The exam aims to evaluate the knowledge acquired and the student's ability to solve biological problems based on the topics covered in the course.
BIO/06 - COMPARATIVE ANATOMY AND CYTOLOGY
BIO/13 - EXPERIMENTAL BIOLOGY
BIO/13 - EXPERIMENTAL BIOLOGY
Lessons: 48 hours
Professor:
Messina Graziella
Shifts:
-
Professor:
Messina GraziellaProfessor(s)
Reception:
On appointment (email)
Torre C 7th floor