The objectives of the biochemistry teaching are: 1. to provide the knowledge necessary to an understanding of the structure and function of the main biological molecules constituting living organisms; 2. to describe the main biological processes present in animal cells, with particular regard to the metabolic fates of food components (carbohydrates, lipids and proteins); 3. to investigate the molecular mechanisms through which monogastric and ruminant animals obtain energy from food in aerobic and anaerobic conditions; 4. to acquire basic knowledge relating to DNA replication and RNA and protein biosynthesis processes.
Expected learning outcomes
1- Knowledge and understanding: The course is aimed at providing information concerning (a) the structure and function of the most relevant biomolecules, including macromolecules (b) the main biological processes operating in animal cells, including the biological fate of the main components of nutrients, mechanisms of energy production and storage and (c) expression of genetic information. 2-Ability to apply knowledge and understanding: At the end of the course the student must be able to distinguish the main metabolic effects of the proteic, lipidic and glicidic components of food, with particular emphashis on their relative role in energy production and storage under aerobic or anaerobic conditions. This basic knowledge will be indispensable in the teachings of subsequent years, related to animal welfare
Protein structural levels (2 hours) Collagen (2 hours) Membranes and membrane transport(2 hours) Myoglobin and hemoglobin (2 hours) Enzymes, mechanism and regulation of enzyme action. Zymogen (2 hours) Molecular mechanism of digestion, absorption and transport of biomolecules (2 hours) Metabolism and bioenergetics(2 hours) Carbohydrates metabolism: glycolysis, pentose phosphate pathway, glycogen, gluconeogenesis, synthesis of lactose (4 hours) TCA cycle and oxidative phosphorylation (2 hours) Fatty acid catabolism and lipid biosynthesis. Ketone bodies. Cholesterol metabolism (4 hours) Amino acid metabolism, ammonia metabolism and urea cycle (2 hours) Nucleotides: purinies and pyrimidines metabolism (2 hours) Metabolic integration and intra-extra cellular regulation (2 hours) Nucleic acids and biological function. Genetic code. DNA replication, transcription, gene expression and protein synthesis (10 hours) Rumen biochemistry (6 hours) Lab techologies (2 hours)
Prerequisites for admission
Good basic knowledge of organic chemistry is recommended.
6 CFU (48 hours) lectures
Nelson/Cox: INTRODUZIONE ALLA BIOCHIMICA DI LEHNINGER (Zanichelli) Campbell/Farrell: BIOCHIMICA (EdiSES) Educational material available on the Ariel teaching platform
Assessment methods and Criteria
The final test consists of a written exam with 5 questions concerning the various topics of the program. It will be assessed in thirtieths and the final grade will take into account the accuracy and quality of the answers, as well as the proper use of the specific language of the discipline.