Biomolecular Methods

The program includes the following topics:
- How to work in a laboratory of molecular biotechnology (safety, behavior, equipment, etc.).
- Preparation of solutions (buffer systems, biological buffers, etc.).
- Solubility of proteins and nucleic acids (extraction and precipitation)
- Spectrophotometric techniques (quantification and spectroscopic analysis of nucleic acids and proteins, fluorimetry).
- Electrophoretic techniques: one- and two-dimensional electrophoresis of proteins, electrophoresis of nucleic acids (genomic DNA, restriction fragments, RNA), blotting.
- Immunochemical techniques (Western and dot blotting, ELISA).
- Chromatographic techniques: traditional and MiniSpin (gelfiltration, ion exchange, hydrophobic interaction, affinity).
- Protein and nucleic acids sequencing
- Essays of enzyme activity.
- The enzymes in molecular biology (nucleases, ligases, polymerases, modifying enzymes).
- Restriction enzyme analysis
- PCR: end-point and real-time (design of oligonucleotides, optimum temperature, titration of MgCl2).
- Ligation of a restriction fragment in a plasmid cloning. Transformation and blue-white screen.
- Bioinformatics: bioinformatics in gene cloning, PCR and protein studies (databases, search of sequences for alignment, position in the genome, etc.).

[Program for not attending students with reference to descriptor 1 and 2]:
The program includes the following topics:
- How to work in a laboratory of molecular biotechnology (safety, behavior, equipment, etc.).
- Preparation of solutions (buffer systems, biological buffers, etc.).
- Solubility of proteins and nucleic acids (extraction and precipitation)
- Spectrophotometric techniques (quantification and spectroscopic analysis of nucleic acids and proteins, fluorimetry).
- Electrophoretic techniques: one- and two-dimensional electrophoresis of proteins, electrophoresis of nucleic acids (genomic DNA, restriction fragments, RNA), blotting.
- Immunochemical techniques (Western and dot blotting, ELISA).
- Chromatographic techniques: traditional and MiniSpin (gelfiltration, ion exchange, hydrophobic interaction, affinity).
- Protein and nucleic acids sequencing
- Essays of enzyme activity.
- The enzymes in molecular biology (nucleases, ligases, polymerases, modifying enzymes).
- Restriction enzyme analysis
- PCR: end-point and real-time (design of oligonucleotides, optimum temperature, titration of MgCl2).
- Ligation of a restriction fragment in a plasmid cloning. Transformation and blue-white screen.
- Bioinformatics: bioinformatics in gene cloning, PCR and protein studies (databases, search of sequences for alignment, position in the genome, etc.).