Diagnostic Techniques in Microbiology, Virology, Micology and Parasitology

A.Y. 2019/2020
4
Max ECTS
40
Overall hours
SSD
MED/07 MED/46
Language
Italian
Learning objectives
At the end of the course the student will:
1. Possess a theoretical-practical knowledge of the most common laboratory diagnostic techniques applied in bacteriology, virology, mycology AND parasitology.
2. Know the general organizational criteria of the Clinical Microbiology laboratory, the correct flow of procedures, the analytical control of the instruments, the methods and the related reagents, and the specific safety criteria in the Microbiology laboratory
Expected learning outcomes
Student will be able to apply the most relevant analytical procedures in clinical microbiology. He will also learn the most important diagnostic algorithm in the field of clinical microbiology.
At the end of the course the student will be able to perform the most basic microbiological tests for a correct diagnosis. She/he will apply techniques including the basic gram-stain till the most relevant and difficult diagnostic assays.
Course syllabus and organization

Single session

Prerequisites for admission
The student must have knowledge in the field of microbiology and how to categorize and classify microorganisms on the basis of their related diseases. He/she will therefore need to know the main viruses, bacteria and fungi of medical interest, as well as the technical bases for their phenotypic and genotypic identifications.
Assessment methods and Criteria
The exam will be both in a written and oral form by including all the topics listed in the program. In particular, each student will receive a written test with open questions and a subsequent oral examination, both supported on the same day.
Microbiologia e microbiologia clinica
Course syllabus
BACTERIOLOGICAL EXAMINATION AND DIFFERENTIAL STAININGS METHODS
PREVENTION OF BIOLOGICAL RISK:
∙ Review of the use of individual protection devices, air flow cabinets management and immunological surveillance.
PRINCIPLES OF VIROLOGICAL DIAGNOSYS:
∙ CULTURAL EXAMINATION;
∙ tissue and cell cultures;
∙ Direct and indirect immunofluorescence tests for identification of specific virus antigens
∙ Molecular Biology Techniques
SEROLOGICAL DIAGNOSIS AND CONFIRMATORY TESTS.
URINARY TRACT INFECTIONS:
∙ MAIN PATHOGENES;
∙ Laboratory diagnosis.
INFECTIONS OF THE GASTROINTESTINAL TRACT:
∙ MAIN PATHOGENES;
∙ Laboratory diagnosis.
MICROBACTERIA AND M.TUBERCULOSIS:
∙ Diagnosis of infection (culture test, microscopic examination and PCR).
RESPIRATORY TRACT INFECTIONS:
MAIN PATHOGENES;
∙ Laboratory diagnosis.
INFECTIONS OF THE CENTRAL NERVOUS SYSTEM: - main pathogens;
∙ Laboratory diagnosis.
GENITAL TRACT INFECTIONS:
∙ MAIN PATHOGENES;
∙ Laboratory diagnosis.
DIAGNOSTICS OF NON-VIRAL SEXUALLY TRANSMITTED MICROORGANISMS:
MYCOPLASMA, CHALAMYDIA TRACHOMATIS, NEISSERIA GONORRHOEAE:
∙ Laboratory diagnosis
DIAGNOSTIC PROTOCOLS FOR INFECTIONS RELATED TO VASCULAR CATHETER.
VIRUS OF HEPATITES A, B, C AND D AND VIRUS HIV:
∙ Main characteristics and laboratory diagnosis
Teaching methods
Frontal lessons assisted by visual tools as well as practical and technical approaches.
Exercises at the Clinical Microbiology Laboratory.
These methods will guide the student to the best conditions for learning clinical microbiology and the main techniques related to.
By slides showing and procedural/practical laboratory tests during the lessons, each student will be provided with all the tools necessary to pass the exams.
Teaching Resources
Michele La Placa: Principi di Microbiologia Medica, Edises s.r.l., Napoli
Patrick R. Murray, Ken S. Rosenthal, Michael A. Pfaller: Microbiologia Medica, Edra Masson, Milano
Scienze tecniche di medicina di laboratorio
Course syllabus
TYPE AND TREATMENT OF MICROBIOLOGICAL SAMPLES
∙ Physiologically sterile and non-sterile human collecting materials
∙ Sampling method
∙ Transportation and storage methods
∙ Evaluation of conformity of the samples
TREATMENT AND PROCESSING OF ANAEROBES:
∙ Processing mode
∙ Anaerobic maintenance
BRONCHIAL ASPIRATE AND ESCREATE
∙ Processing techniques
∙ Microorganisms involved
CATHETERS
∙ Urinary tract
∙ Processing techniques
∙ Microorganisms involved
∙ Blood system
∙ Processing techniques
FACAL SAMPLES
∙ Processing techniques
∙ Microorganisms involved
URINE
∙ Processing techniques
∙ Microorganisms involved
BLOODCULTURE AND LIQUOR
∙ Processing techniques
∙ Microorganisms involved
ESSUDATES AND RELATED MATERIALS
∙ Processing techniques
∙ Microorganisms involved
PROSTHETIC SAMPLES
∙ Processing techniques
∙ Microorganisms involved
ORO-FARINGAL SAMPLES
∙ Processing techniques
∙ Microorganisms involved
VAGINAL SAMPLES
∙ Processing techniques
∙ Microorganisms involved
Teaching methods
Frontal lessons assisted by visual tools as well as practical and technical approaches.
Exercises at the Clinical Microbiology Laboratory.
These methods will guide the student to the best conditions for learning clinical microbiology and the main techniques related to.
By slides showing and procedural/practical laboratory tests during the lessons, each student will be provided with all the tools necessary to pass the exams.
Teaching Resources
Michele La Placa: Principi di Microbiologia Medica, Edises s.r.l., Napoli
Patrick R. Murray, Ken S. Rosenthal, Michael A. Pfaller: Microbiologia Medica, Edra Masson, Milano
Microbiologia e microbiologia clinica
MED/07 - MICROBIOLOGY AND CLINICAL MICROBIOLOGY - University credits: 2
Lessons: 20 hours
Professor: Drago Lorenzo
Scienze tecniche di medicina di laboratorio
MED/46 - BIOTECHNOLOGY AND METHODS IN LABORATORY MEDICINE - University credits: 2
Lessons: 20 hours
Professor: Clerici Pierangelo