Imaging techniques in bio-medical research

A.Y. 2019/2020
8
Max ECTS
48
Overall hours
SSD
BIO/10 VET/01
Language
English
Learning objectives
The image analysis represents an emerging field of biomedical sciences. This is demonstrated by a growing number of courses available at prestigious universities in the world, as well as by the employment opportunities and the increasing use of these technologies in scientific publications. With this course, the student collects, obtains and re-elaborates biological data through the application of physical and mathematical principles of high precision and reproducibility. The data is then processed in order to be appropriately interpreted.
Expected learning outcomes
At the end of the Course, the student will be able to collect and re-elaborate data through the use of novel biotechonological approches based on the image analysis. The results will be evaluated, processed  and appropriately interpreted, using physical and mathematical principles of high precision and reproducibility.
Course syllabus and organization

Single session

Responsible
Lesson period
Second semester
Prerequisites for admission
none
Assessment methods and Criteria
The exam consists of 1 final oral test (50% final mark) and 1 practical test (50% final mark)
Module: Image analysis of cells and tissue
Course syllabus
Basic concepts in imaging. Electromagnetic radiation and interactions with Matter (2 hours),
Optics, including lenses and reflection. Generators, filters and detectors (1 hour)
Biological effects of radiation. Safety considerations (1 hour).
Imaging systems for biological molecole (Proteins, lipids, nucleic acids) (2 hours)
Trasmission microscopy (1 hour)
Scanning microscopy (1 hour)
Phase contrast microscopy (1 hour)
Fluorescence microscopy (1 hour)
Confocal and multiphoton microscopy (2 hours)
Imaging of live and fixed cells (2 hours)
Fluorescence Resonance Energy Transfer (2 hours)
Use of radioisotopes for imaging con radionuclide (1 hour)
Magnetic resonance (1 hour)

Practical training total hours

Time-lapse of living systems 2
Use of algorithms used in imaging 1
Processing, digitalization deconvolution 1
Image degradation, Spatial resolution, Signal and noise 1
Image measurements, histograms 1
Use of Image 1.62 NIH program 2
Use of Leica Confocal System 2
Use of Nikon NKM Program 2
Teaching methods
Lectures anf practicals
Teaching Resources
Handouts
Module: MALDI imaging
Course syllabus
Sample preparation 4
MALDI imaging 4
Proteins identification and analysis 4
Teaching methods
The course consists of frontal and practical lessons
Teaching Resources
References and Internet links will be available
Module: Molecular Imaging
Course syllabus
Preliminary overview 4
Relaxation Theory and Mechanisms
Magnetic properties and image effects
Extracellular fluid agents 2
Organ-specific agents 2
Ultrasound theory and demonstration 3
Bioluminescent imaging and the use of reporter in vivo 2
Teaching methods
Lectures anf practicals
Teaching Resources
References and Internet links will be available
Module: Image analysis of cells and tissue
VET/01 - VETERINARY ANATOMY - University credits: 4
Lessons: 24 hours
Professor: Brevini Tiziana
Module: MALDI imaging
BIO/10 - BIOCHEMISTRY - University credits: 2
Lessons: 12 hours
Module: Molecular Imaging
VET/01 - VETERINARY ANATOMY - University credits: 2
Lessons: 12 hours
Professor: Brevini Tiziana