Molecular and Cellular Imaging
A.Y. 2019/2020
Learning objectives
The aim of the course is to present different techniques of microscopy (optical, electronic and scanning probe) also applied in lab-on-a-chip systems.
After following this course, the students will acquire basic know-how in microscopy and the capability to select suitable approaches to image biological samples.
The course is ideally linked to those dealing with the need of imaging, i.e. molecular and cellular biology, etc.
After following this course, the students will acquire basic know-how in microscopy and the capability to select suitable approaches to image biological samples.
The course is ideally linked to those dealing with the need of imaging, i.e. molecular and cellular biology, etc.
Expected learning outcomes
Undefined
Lesson period: First semester
Assessment methods: Esame
Assessment result: voto verbalizzato in trentesimi
Single course
This course cannot be attended as a single course. Please check our list of single courses to find the ones available for enrolment.
Course syllabus and organization
Single session
Responsible
Lesson period
First semester
Course syllabus
The course provides description of the imaging techniques applied to biology. Some basic notions of geometrical optics are functional to detailed description of optical microscopes. Confocal microscopy and related techniques (FRAP, FRET, FLIM) will be extensively treated together with more recent developments in super-resolution techniques, in particular SIM, PALM, STORM, STED. Some practical experience in cell staining will be given. Electron microscopies (SEM, TEM, Cryo-TEM) will be presented underlying the different investigations on biological samples that can be carried out. Atomic Force Microscopy (AFM) will be also presented as a versatile approach for measuring mechanical properties of biological samples.
The course will be concluded with a part focused on lab-on-a-chip and lab-on-cell systems for high throughput analyses, disposable and low-cost devices as promising applications in biotechnology.
During the course some visits to facilities equipped with the described instruments will be organized. In a laboratory of microfluidics practical experience will be carried out on micro-devices in the contest of lab-on-a-chip design.
The course will be concluded with a part focused on lab-on-a-chip and lab-on-cell systems for high throughput analyses, disposable and low-cost devices as promising applications in biotechnology.
During the course some visits to facilities equipped with the described instruments will be organized. In a laboratory of microfluidics practical experience will be carried out on micro-devices in the contest of lab-on-a-chip design.
Prerequisites for admission
Basic knowlegde of Classic Electromagnetism and Optics.
Teaching methods
Teaching mode: classroom lectures supported by projected material, visits to the laboratories equipped with the microscopes. Practical sessions in microfluidic lab.
Attendance: highly recommended.
Attendance: highly recommended.
Teaching Resources
Copies of the slides projected in the classroom as well as other materials will be made available through the course website on the ARIEL platform of the University of Milano (http://ariel.ctu.unimi.it). By no means this material replaces the lectures or a textbook. The material is made available only to registered students of the Degree Course in Molecular Biotechnology and Bioinformatics and should not be distributed to others.
http://clenardib.ariel.ctu.unimi.it/v5/home/Default.aspx
[1] G. Haugstad, Atomic force microscopy: understanding basic modes and advanced applications, John Wiley & Sons, Hoboken, N.J, 2012.
[2] U. Kubitscheck, ed., Fluorescence microscopy: from principles to biological applications, Wiley-Blackwell, Weinheim, 2013.
[3] R.F. Egerton, Physical principles of electron microscopy: an introduction to TEM, SEM, and AEM, second edition, Springer, Cham, 2016.
http://clenardib.ariel.ctu.unimi.it/v5/home/Default.aspx
[1] G. Haugstad, Atomic force microscopy: understanding basic modes and advanced applications, John Wiley & Sons, Hoboken, N.J, 2012.
[2] U. Kubitscheck, ed., Fluorescence microscopy: from principles to biological applications, Wiley-Blackwell, Weinheim, 2013.
[3] R.F. Egerton, Physical principles of electron microscopy: an introduction to TEM, SEM, and AEM, second edition, Springer, Cham, 2016.
Assessment methods and Criteria
The evaluation of the student's performance is based on: i) the presentation of a paper dealing with recent studies in biotechnology using at least one of the imaging techniques described in the course and ii) oral examination spanning all the topics covered in the course.
Examples of the examination test will be discussed during classes and made available to students.
Examples of the examination test will be discussed during classes and made available to students.
FIS/03 - PHYSICS OF MATTER
FIS/07 - APPLIED PHYSICS
FIS/07 - APPLIED PHYSICS
Lectures: 48 hours
Professors:
Lenardi Cristina, Santaniello Tommaso
Shifts:
-
Professors:
Lenardi Cristina, Santaniello TommasoProfessor(s)