Genetics, Cellular and Molecular Biology

A.Y. 2020/2021
12
Max ECTS
96
Overall hours
SSD
BIO/11 BIO/13 BIO/18
Language
English
Learning objectives
The course introduces the fundamentals concepts in Cellular Biology, Molecular Biology, and Genetics.
Expected learning outcomes
Students will acquire an operative understanding on genetics, cellular, and molecular biology fundamentals and in order to be able to apply the knowledge acquired to the interpretation of the results of the bioinformatic analysis of biomolecular data.
Course syllabus and organization

Single session

Responsible
Lesson period
First semester
Lessons will be held exclusively on line on the Microsoft Teams platform, and students will be able to follow them both in real time according to the course timetable, or through the recordings that will be made available to them.
Course syllabus
Cellular Biology:

1: Outer cellular structures: Biological membranes, cell walls and flagella
2: Cell biology of Anabolism: The Nucleus, the Endoplasmic Reticulum and the Golgi apparatus
3: Cell biology of Catabolism: The endo-lysosomal system and autophagy
4: Cellular energy and detox: Mitochondria, Chloroplasts and Peroxisomes
5: Intracellular logistics: The cytoskeletons, transport routes and motor systems
6: Elements of multicellular organization: From biofilms to cell specialization, cell-cell communication and stem cells
7: The cell in time: Cell cycles, cell death and senescence
8: Organelles and disease + Wrap up and synthesis

Genetics:

1: Introduction to Genetics
2: Mendelian Genetics I
3: Mendelian Genetics II
4: Principles of human genetics
5: Exercises
5: The structure of DNA, and Chromosomes.
6: The structure of chromatin
7: The structure of genes.
8: The genetic code.
9: Mutations and polymorphisms
10: Models of regulation (the Operon model)
11: Hybridization, amplification and NGS techniques.
12: Cancer Genetics
13: Population Genetics
14: Exercises
15: Principles of Epigenetics/Imprinting

Molecular Biology:

1- Genes and genome
2- DNA replication
3- Repair Mechanisms
4- Transcriptional regulation in procaryotes
5-Transcriptional regulation in eukaryotes
6- Splicing mechanisms
7- mRNA processing
8- Translation and translational regulation
9- Post- translational regulation
10- Molecular mechanisms of cell-cycle
11- ncRNA
12- Methods- plasmids and cloning
13- Methods- Northern, Southern, Western, PCR
14- Methods- DNA insertion in cell culture and different animal modes
15- Methods- CRISPR-CAS9, genome editing
Prerequisites for admission
No prerequisites different from those required for admission to the Master Degree program.
Teaching methods
Lectures
Teaching Resources
Slides used during classes will be shared with students.
Textbooks covering the topics of the classes will be suggested by teachers.
Assessment methods and Criteria
Written and oral tests.
BIO/11 - MOLECULAR BIOLOGY - University credits: 0
BIO/13 - EXPERIMENTAL BIOLOGY - University credits: 0
BIO/18 - GENETICS - University credits: 0
Lectures: 96 hours
Professor(s)
Reception:
Thursday 17-18 (by appointment)
office 2b