Imaging techniques for biomedical applications

A.Y. 2020/2021
6
Max ECTS
42
Overall hours
SSD
FIS/01 FIS/07
Language
Italian
Learning objectives
The main objective of the course is to provide the student with the basic knowledge in imaging techniques with optical, electronic, probe scanning, diffusion-MRI and functional-MRI imaging with reference to applications in the biomedical field. Visits will be made to laboratories equipped with the instruments described in the course. The course also includes a series of seminars of experts in the machine learning and lab-on-chip devices.
Expected learning outcomes
At the end of the Course, the student will learn the fundamental elements of imaging techniques with applications in particular to the biomedical field. The student will acquire the ability to evaluate the most suitable technique / methodology for structural and functional imaging of biological samples at the level of molecular, cellular, and tissue structures up to complex organs / organisms.
Course syllabus and organization

Single session

Responsible
Lesson period
First semester
All the lectures will be delivered remotely in synchronous mode.
Course syllabus
Module I
Optical microscopy (CLSM) and FRET, FRAP, FLIM, TIRF techniques
Super-resolution optical microscopy (STED, 2Ph, SIM, PALM)
Electron microscopy (SEM, TEM)
Scanning Probe Microscopy (AFM, STM)
Lab-on-chip and Cell-on-chip systems

Module II
Fundamental principles of Nuclear Magnetic Resonance
MRI instrumentation
1D MRI:
Space K
Gradient echo
2D / 3D imaging - Spatial decoding - Image weighing
2D / 3D MRI sequences
FT continuously
Radon Transform and Reconstruction of images with projection and rear projection techniques
Outline of functional MRI
Introduction to Diffusion MRI
Outline of Quantitative MRI
MRI Contrast Agents, Nanomagnetism and its applications in medicine
Prerequisites for admission
None
Teaching methods
All the theoretical and practical lectures will be delivered synchronously on ZOOM platform. Slides on Ariel.
Teaching Resources
Slides on Ariel site

Module I
A. Diaspro et al. - Nanoscopy and Multidimensional Optical Fluorescence Microscopy. ed. Chapman and Hall/CRC
J.J. Bozzola et L. D. Russel - Electron Microscopy. Jones & Bartlett Learning

Module II
R. W. Brown et al., Magnetic Resonance Imaging: Physical Principles and Sequence Design
Assessment methods and Criteria
The evaluation of the student's performance is based on: i) the presentation of a paper dealing with recent studies using at least one of the techniques of imaging described in the course and ii) oral examination spanning all the topics covered in the course.
Examples of the examination test will be discussed during classes and made available to students.
FIS/01 - EXPERIMENTAL PHYSICS - University credits: 0
FIS/07 - APPLIED PHYSICS - University credits: 0
Lessons: 42 hours