Research and development of new diagnostic and therapeutic metodologies

A.Y. 2020/2021
Overall hours
BIO/11 MED/08 MED/15 MED/36
Learning objectives
The aims of this course will be to provide an up-date knowledge of novel, emerging basic and technical aspects in the diagnosis and therapeutic approaches in cancer.
In particular, the course will be focused on mouse models, organoids, relevance of NGS in the context of tumor heterogeneity, the emerging role of non coding RNAs as therapeutic platforms, the advance of minimal residual disease approaches in the management of patients in the era
of new drugs, and the physical bases and possible applications of the most important imaging techniques
Expected learning outcomes
In particular, by following this course it will be possible to learn about:
Animal models as diagnostic and therapeutic tools
Rationale for NGS studies in cancer
The clinical relevance of the non coding RNAs
Molecular monitoring of hematological malignancies
Imaging techniques for clinical and pre-clinical oncological studies
Course syllabus and organization

Single session

Lesson period
Third trimester
All classes planned during the third trimester of the first year of the Master Degree Medical Biotechnology and Molecular Medicine, will be given as frontal teaching and distance synchronous learning. In case of a new suspension of frontal lectures, all students will be asked to move to distance synchronous learning.
Each hour of lesson will be divided into 45' of formal teaching followed by 15' of discussion/interaction/question time. All lessons will be recorded and made available for students on the indicated platforms (i.e. Ariel, TEAMS, etc).
Unless otherwise specified, exams will be organized in classroom but if the student is unable to reach the building for COVID-related issues, other forms will be considered (this apply for exams scheduled until the 31st July - 2021).
Course syllabus
The pathology lab: a platform for patients' diagnosis and therapy
Technologies and methods for assessing predictive factors in cancer patients.
Molecular follow-up of tumor resistance: the liquid biopsy model.
Pathology in the multidisciplinary team: from morphology to genome.
Main Diagnostic Imaging procedures and their applications. RX/CT and Ultrasound: Principles and methods for ultrasound imaging; clinical and pre-clinical applications. Magnetic Resonance Imaging (MRI): methodology, and advanced applications. Nuclear based techniques: Principles and methods of single photon emission tomography (SPECT), positron emission tomography (PET) imaging procedures and main tracers for oncological imaging. Introduction to Radiomics.
Blood diseases:
Clonal heterogeneity in blood cancers by NGS Novel classifications and their biological and prognostic relevance in hematological tumors based on NGs study Use of murine models for the study of mutations affecting splicing factors in the pathogenesis of myeloid disorders. Monitoring the minimal residual disease in hematological neoplasms: technical advances and clinical implications Non coding RNA in blood cancers: from genetic associations, molecular mechanisms, and therapeutic strategies.
Molecular biology:
Patient-specific tumor models: from xenografts to organoids. Cancer reprogramming: from mechanism to tool for the dissection of tumor pathogenesis and the identification of draggable pathways Cancer-specific epigenetic dysregulation and its therapeutic application.
Prerequisites for admission
The student must have acquired the general concepts of the basic radiation physics, of biological and molecular pathogenetic mechanisms of human diseases and the basic laboratory techniques for the study of cellular functions, phenotype and genome.
Teaching methods
The teachers will use frontal lessons with visual support material.
The teachers will use the Ariel platform to provide the teaching material which consists of a copy of the presentations used in the lectures and other educational material. Attendance to lectures and disciplinary laboratory activities is mandatory
Teaching Resources
Students will receive pdf files of the presentations used during the lectures.
Moreover review articles will be available on the different topics.
Assessment methods and Criteria
The exam will be a written test on the topics listed in the programme. In particular, each student will be given a test consisting of 6 open questions for the duration of one and a half hours and the mark will be expressed in thirtieths. Each student will be evaluated for the knowledge of the subject, the ability to organize the answer in a discursive way and the competence in the use of specialist vocabulary. Each student will be provided with all the tools necessary for passing the exam.
Blood diseases
MED/15 - BLOOD DISEASES - University credits: 2
Lessons: 14 hours
Molecular biology
BIO/11 - MOLECULAR BIOLOGY - University credits: 2
Lessons: 14 hours
Professor: Testa Giuseppe
MED/08 - PATHOLOGY - University credits: 1
Lessons: 7 hours
Professor: Pruneri Giancarlo
MED/36 - IMAGING AND RADIOTHERAPY - University credits: 1
Lessons: 7 hours
Professor: Ottobrini Luisa