Methods in pharmacology and toxicology

A.Y. 2021/2022
Overall hours
Learning objectives
The course aims at introducing students to the main pharmacological and toxicological methodologies used to test drugs and biologicals in the cardiometabolic field.
Expected learning outcomes
Students attending the course in Pharmacological Methodologies will be able to evaluate the regulations
for the use of experimental models and those for clinical and genetic studies. Students will be able to
understand the efficacy and safety of the drug in the preclinical and clinical phases, to discuss critical
aspects of pharmacokinetic.
Moreover they will learn key concepts of imaging techniques in vascular biology for the study of obesity
and metabolic diseases. Finally the different omics strategies used in pharmacology with particular
reference to cardiometabolic diseases will be presented.
Course syllabus and organization

Single session

Lesson period
Second semester
The lessons will be held in attendance or through Microsoft Teams, Zoom Meeting or other platforms in asynchronous or synchronous transmission.
The program and the teaching material will be the same.
The exam will be oral using Microsoft Teams, Zoom Meeting or other platforms; if allowed by the emergency rules, it might be in presence as oral.
Course syllabus
The teaching aims to introduce students to the main pharmacological and toxicological methodologies used for the experimentation of the active drugs in the cardiometabolic field.
-Regulation and guidelines for the use animal models in experimental research.
-Regulation and guidelines for clinical and genetic studies.
-Study of cardiovascular and immunometabolic diseases.
-Animal models for the study of obesity and metabolic diseases.
-Animal models for the study of atherosclerosis.
-Platelet aggregation and models for the study of arterial thrombosis.
-Experimental approaches for the study of angiogenesis.
-Immune and autoimmune diseases.
-Use of cytofluorimetry techniques for the study of leukocyte population
-Investigation of oxidative stress in the heart.
-Imaging procedures in vascular biology.
-Imaging procedures in metabolic disorders and obesity.
-Clinical Studies
-Biotechnological drugs
-From gene identification to target therapies
-Tests for mutagenesis
-Tests for cancerogenesis
-Reproduction toxicity
-Drug toxicity
Prerequisites for admission
Good knowledge of anatomy, cell biology, biochemistry and general pharmacology are required. Also a good understanding of English. The frequency is strongly recommended. Ad interim tests will be organized during the course.
The final test examination is written and focuses on the topics discussed in the course.
Teaching methods
Interactive teaching and specific lessons with ppt
Teaching Resources
Tossicologia molecolare e cellulare. Paoletti et al , UTET
Tossicologia. Helmut Greim, Zanichelli
Elementi di Tossicologia. Casarett and Doull, Publishing House Ambrosiana
Farmacologia: Principi di base e applicazioni terapeutiche. Rossi, Cuomo and Riccardi, Minerva Medica Edition
Assessment methods and Criteria
On going evaluations and final exam on the topics discussed during the course
BIO/14 - PHARMACOLOGY - University credits: 6
Lessons: 48 hours