Ree Crispres - a Workshop On Genome Editing Technologies
A.Y. 2021/2022
Learning objectives
This supplementary activity provides students with skills in the following areas:
· Identification of target genes for the protection of plants of agricultural interest
· Planning of experiments using genome editing
· Analysis of sequences resulting from genomic editing events
· In vitro cultures
· Phenotyping of the disease
During the pandemic emergency, workshops will be conducted online, based on e-learning and active learning platforms, including virtual labs and gamification.
· Identification of target genes for the protection of plants of agricultural interest
· Planning of experiments using genome editing
· Analysis of sequences resulting from genomic editing events
· In vitro cultures
· Phenotyping of the disease
During the pandemic emergency, workshops will be conducted online, based on e-learning and active learning platforms, including virtual labs and gamification.
Expected learning outcomes
The REE lab will provide 3 CFUs, for a total of 40 hours of class work. The work will be online, during the pandemic emergency. Lectures are provided on a e-learning platform and virtual labs are based on interactive, virtual experiences. The final approval is based on a project that the student independently prepares and presents to the class and the trainers. The REE lab is part of an international training project and is hosting students from two European Universities.
Lesson period: Second semester
Assessment methods: Giudizio di approvazione
Assessment result: superato/non superato
Single course
This course cannot be attended as a single course. Please check our list of single courses to find the ones available for enrolment.
Course syllabus and organization
Single session
Responsible
Lesson period
Second semester
This course may be provided online according to the current sanitary obligations. It includes synchronous and asynchronous activities, that are delivered through MS Teams, Moodle and specific digital-laboratories.
Course syllabus
Day 1 (9.00-17:00): Introduction and workshop on host-pathogen interaction
Day 2 (9:00-17:00): Theory and practice on in plant in vitro culture; Introduction to CRISPR
Day 3 (9:00-17:00): Theory and practice on CRISPR multiplexing
Day 4 (9:00-18:00): Workshop on cloning and rice transformation; Lecture by prof. H. Puchta
Day 5 (9:00-19:00): Theory and practice on off targets; round table and Q&A; social event
A final event organized by the University of Milano takes place in the fall.
Day 2 (9:00-17:00): Theory and practice on in plant in vitro culture; Introduction to CRISPR
Day 3 (9:00-17:00): Theory and practice on CRISPR multiplexing
Day 4 (9:00-18:00): Workshop on cloning and rice transformation; Lecture by prof. H. Puchta
Day 5 (9:00-19:00): Theory and practice on off targets; round table and Q&A; social event
A final event organized by the University of Milano takes place in the fall.
Prerequisites for admission
No formal prerequisites. It is strongly advised to have a basic knowledge in molecular biology.
Teaching methods
The course is part of an international learning experience, conducted in collaboratio with the Universities of Copenhagen and Prague and funded by the 4EU+ project. The students will work in teams with foreign mates. The course is administered remotely, using Microsoft Teams as a communication platform, and Moodle, as a didactic platform. There are lectures and interactive workshops, where the students contribute through a team-work. The workshops are conducted using immersive/interactive videos and digital laboratories.
Teaching Resources
Plant Genome Editing with CRISPR system, methods and protocols. (2019) Y.Qi Editor, doi: 10.1007/978-1-4939-8991-1
The CRISPres Handbook (2021). Published by the CRISPres project.
The CRISPres Handbook (2021). Published by the CRISPres project.
Assessment methods and Criteria
The knowledge acquired by the students is evaluated through a final report, based on a given format, where they describe a project autonomously developed. The students have to present their project during a "knowledge challenge".
AGR/03 - ARBORICULTURE AND FRUITCULTURE
AGR/07 - AGRICULTURAL GENETICS
AGR/12 - PLANT PATHOLOGY
BIO/01 - GENERAL BOTANY
AGR/07 - AGRICULTURAL GENETICS
AGR/12 - PLANT PATHOLOGY
BIO/01 - GENERAL BOTANY
Practicals: 36 hours
Lessons: 6 hours
Lessons: 6 hours
Professor(s)
Reception:
by appointment
via Celoria, 2 - Edificio 10
Reception:
Monday, 10-12 am
Building 21070, first floor