Biobanking

A.Y. 2022/2023
8
Max ECTS
48
Overall hours
SSD
VET/09 VET/10
Language
English
Learning objectives
The aim of the course is to understand the founding principles of current systems of cell, organ and scaffold preservation. Scientific knowledge on cryobiology and cryobanking of germ cells, as a valuable tool for fertility preservation and storage of genetic resources are provided. The standard procedures of organ and homograft procurement and storage and the basic principles that regulate tissue and organ scaffolding, focusing on scaffold sterilization and storage, are also given. Students experience the work in a CryoLab for the application of highly efficient protocols for cryostorage of gametes, embryos and gonadal tissue and practice on homograft preparation and procedures of organ preservation, including organ perfusion, as well as on the principal steps of scaffold preparation and banking.
Expected learning outcomes
At the end of the course students will be able to demonstrate their knowledge on different procedures for banking gametes, embryos, gonadal tissue as well as organs, homografts and scaffolds. Practice in the lab will provide them the basic skills for preparation of samples. Their independent judgment will consist in the ability to evaluate quality of samples that can be subjected to procedures of storage.
Single course

This course cannot be attended as a single course. Please check our list of single courses to find the ones available for enrolment.

Course syllabus and organization

Single session

Responsible
Course syllabus
The integrated course consists of 8 CFU and it is divided in 3 modules:
1. Cryobiology for fertility preservation (3 CFU, Dr. Martina Colombo)
2. Homograft and organ banking 1 (3 CFU, Prof. Fabio Acocella)
3. Homograft and organ banking 2 (2 CFU, Prof. Fabio Acocella)
The program takes place in the second semester.
Fundamentals of cryobiology
Cryopreservation of spermatozoa
Cryopreservation of oocytes/embryos
Cryopreservation of testicular/ovarian tissue
Experience in a CryoLab

Homograft:
- definition, concept and type of
- preparation (Lab)
- storage (Lab)
- evaluation (Lab)
· Organ Banking:
- definition, concept and type of
- preparation (Lab)
- storage (Lab)
- evaluation (Lab)
Scaffold
- definition, concept and type of
- fresh scaffold characteristic (Lab)
- storage (Lab)
- mechanical properties before and after storage
Prerequisites for admission
Basic knowledge of cell and tissues anatomy and physiology
Teaching methods
Attendance is strongly recommended for theoretical lessons and practicals.
In the theoretical lessons students are involved by stimulating their interest and encouraging them to participate by asking questions and asking to summarize parts already discussed in previous lessons.
In practical work, individually or in small groups, students are guided towards the achievement of their autonomy in the manual skills necessary for the execution of the procedures.
Teaching Resources
Scientific papers
Lecture presentations in Ariel
Assessment methods and Criteria
The exam is aimed at ascertaining the knowledge acquired. A single, written exam will take place, based on a practical scenario where a given sample is available and needs to be preserved. Students will be provided with a hypothetical situation and will be requested to describe which procedures they would perform in that particular case and how to perform them, in order to retrieve, process and store such sample for biobanking purposes.
Unit: Cryobiology for fertility preservation
VET/10 - VETERINARY CLINICAL OBSTETRICS AND GYNECOLOGY - University credits: 3
Lessons: 18 hours
Professor: Colombo Martina
Unit: Homograft and organ banking
VET/09 - VETERINARY CLINICAL SURGERY - University credits: 3
Lessons: 18 hours
Professor: Acocella Fabio
Unit: Scaffold banking
VET/09 - VETERINARY CLINICAL SURGERY - University credits: 2
Lessons: 12 hours
Professor: Acocella Fabio
Educational website(s)